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I. Introduction:This assay uses a lawn of phage-susceptible E. coli (DH10B) embedded in a layer of agarose. This top agarose lays on a bed of standard LB agar. If phage is present in a clone streaked or stamped onto the top agarose lawn, the lawn there will be lysed on incubation overnight. II. Materials and Equipments:DH10B (original glycerol stock) or DH10B cultured stock for phage t......阅读全文
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