ELISPOTProtocol
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level. The popularity of this assay has seen resurgence in recent years as researchers attempt to gain a better understanding of immune responses in a variety of applications. The following protocol is an example of a typ......阅读全文
ELISPOT－protocol
实验概要The procedure below is a general guideline procedure for ELISPOT. Abcam ELISPOT kits have been designed for detection of various cytokines and g
ELISPOT－Protocol
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on t
ELISPOT－Protocol
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on t
ELISPOT
实验材料 抗原试剂、试剂盒 PBSPRMI1640培养基氯化铵仪器、耗材 PVDF膜96孔板实验步骤 1. PBS 溶解抗原为30 μg/ml，加100 μl/孔于PVDF 膜铺底的96 孔灭菌板过夜； 2. 第二天吸去包被液后，加5％FCS 的PRMI 1640 培养基100 μl 封闭1 小时，
ELISPOT
随着酶联免疫分析技术在医学及生物学领域的广泛应用，使体外检测各种细胞因子及抗体研究有了新的突破。在研究免疫应答机制时以往常用用酶联免疫吸附法（ELISA）检测体液中游离的细胞因子（CK）或抗体，但由于游离的循环抗体或CK的半哀期不同，使之在体液中不断的被代谢或与靶器官结合，而不能确切的反映体内的抗体
The－Impact－of－Harmo...－（一）
实验概要During more than 25 years of application in immunological sciences, ELISPOT has been established as a routine, robust, versatile, and reliable a
Dual－ELISPOT
实验概要We provide a protocol using an FITC-conjugated primary antibody and a biotinylated primary antibody. Those are in turn recognized by anti-FITC H
The－Impact－of－Harmo...－（二）
In addition, proficiency panel projects have demonstrated that even after an experiment was done and spots were counted, considerable variation can
ELISpot实验操作流程
ELISPOT全名为酶联免疫斑点检测，英文：Enzyme-linked Immunospot Assay。它结合了细胞培养技术与酶联免疫吸附技术（即ELISA技术），能够在单细胞水平检测细胞因子的分泌情况。其技术原理，一句话概括就是：用抗体捕获培养中的细胞分泌的细胞因子，并以酶联斑点显色的方式将其表
NAi－protocol
siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western
Immunoblot－Protocol
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
RNAi－protocol
siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western
PCR－protocol
PCR reactionProtocol for 50µl reaction - adjust amounts if necessary, for a 20µl reaction use the same volumes of primer and dNTP-mix, but adjust the
Immunoprecipitation－Protocol
实验概要Immunoprecipitation is a procedure by which proteins or peptides that react specifically with an antibody are removed from solution and examined
ELISA－protocol
ELISA protocol：1.取5－10ul BMMY表达上清用0.05M NaHCO3稀释到100ul铺ELISA板，37度或室温振荡大于1小时。注意一定要做一个GS115空菌株表达上清作为阴性对照，最好还找一个带有histag的蛋白作为阳性对照。2.TPBS洗板3次，方法：倒掉铺板液，倒置于
RLGS－protocol
A. Preparation of DNA SolutionIn the case of rice, for example This method may be appllicable for many grass species and some other plants.
ELISPOT－(Enzymelinked－ImmunoSPOT)
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, bu
ELISPOT与ELISA的区别
1.ELISA通过显色反应，在酶标仪上测定吸光度，与标准曲线比较得出可溶性蛋白总量。2.ELISPOT通过显色反应，在细胞分泌这种可溶性蛋白的相应位置上显现清晰可辨的斑点，可直接在显微镜下或通过仪器对斑点进行计数，1个斑点代表1个细胞，从而计算出分泌该蛋白的细胞的频率。3.由于是单细胞水平检测，EL
酶联免疫斑点实验(ELIspot)——酶联免疫斑点实验(ELIspot)
实验材料血液样品细胞样品试剂、试剂盒70％乙醇PBS脱脂奶粉链霉亲和素检测抗体包被抗体仪器、耗材PVDF膜96孔培养板硝基纤维素底板免疫斑点板酶标板实验步骤一、包被96孔板 用70％乙醇浸润96孔板中的PVDF膜30 s 加入捕获抗体（PBS稀释），4℃过夜 倒空板中包被液，轻轻在纸上拍干，用PBS
ELISPOT（酶联免疫斑点法）技术
酶联免疫斑点技术(Enzyme Linked Immunospot Assay, ELISPOT)是细胞免疫学研究中最敏感的检测方法之一，可以在单细胞水平对抗体分泌细胞(ASC)及细胞因子(CK) 分泌细胞进行检测。由于是单细胞水平检测，ELISPOT比ELISA 和有限稀释法等具有更高的灵敏性，能
酶联免疫斑点实验(ELIspot)_酶联免疫斑点实验(ELIspot)2
实验方法原理ELISPOT技术原理，一句话概括就是：用抗体捕获培养中的细胞分泌的细胞因子，并以酶联斑点显色的方式将其表现出来。实验材料细胞试剂、试剂盒PBSPBST乙醇包被抗体封闭液抗体稀释液检测抗体酶联亲和素AEC显色液PHA刺激物U-Cytech无血清培养基仪器、耗材ELISPOT读数仪超净工作
Transformation－Protocol－for－Arabidopsis
Transformation Protocol for Arabidopsis – AbbreviatedGerminate seed in pots↓ 4 weeksStreak bacteria onto YM/MinA↓ 2-3 days 28°CSpray/dip bacterial sus
Microarray－Hybridization－Protocol
Introduction:One microarray set consists of 7 nylon membranes with 2.5 x 7.5 cm dimension. 2304 genes were spotted onto nylon membranes (Schleicher an
Nuclear－Extraction－Protocol
实验概要The procedure presented below describes a method for extracting nuclear from several cell lines of human origin.主要试剂Hypotonic Buffer Solution20 mM
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
RNA－Isolation－Protocol
Stabilize RNAStart with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up)Pipet 30 ml of RNAProtect Bacteria Reagen
Yale－Immunofluorescence－Protocol
实验概要We provide a protocol for fixation, immunostaining, and imaging in 384-well Plates.主要试剂Reagents1. 384-well view plates (Aurora)2. HUVEC (pooled, L
Bacteria－Culture－Protocol
Bacteria Culture ProtocolBy 徐晓政1、TBS Medium Preparation:Prepare 1L of TBS medium contains:Tryptone 12gYeast extract 24gNaCl 5gSodium Succinate 5gGlyce
Intracellular－Staining－Protocol
1. Fix cells- Add16% formaldehyde directly into culture medium to obtain a final concentration of 1.5% formaldehyde.2. Incubate in fixative for 10 min
Histone－blotting－protocol
实验概要 Western blot detection of histone proteins. 实验步骤 The following protocol refers to the western blot detection of histone proteins derived from p