RedBloodCellLysisProtocols
实验概要BioLegend’s Red Blood Cell (RBC) Lysis Buffer (Cat. No. 420301) has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions of hematopoietic tissues, such as spleen, as well as RBCs derived from peripheral blood. RBC Lysis Buffer contains ammonium chloride, which has been demonstrated to lyse RBCs with a minimum of harmful effects on lym......阅读全文
Red－Blood－Cell－Lysis－Protocols
实验概要BioLegend’s Red Blood Cell (RBC) Lysis Buffer (Cat. No. 420301) has been designed, formulated, and tested to ensure optimal lysis of RBCs in sin
Protocol－for－Isolating－DNA－from－Blood－with－Nucleated－Red－blood－Cells
实验概要DNA isolation from fish or avian blood sample can be difficult because it contains nucleated red blood cells. E.Z.N.A. NRBC Blood DNA Kit is de
Vacuum/Spin－Protocol－for－Isolating－DNA－from－Blood－with－Nucleated－Red
实验概要DNA isolation from fish or avian blood sample can be difficult because it contains nucleated red blood cells. E.Z.N.A. NRBC Blood DNA Kit is de
Human－Peripheral－Blood－Mononuclear－Cell－Preparation
This protocol describes a procedure for isolating human peripheral blood mononuclear cells (lymphocytes and monocytes) from a Buffy Coat (obtained fro
Method:－Lymphoblastoid－Cell－Lines－from－Frozen－Whole－Blood
Method: Lymphoblastoid Cell Lines from Frozen Whole BloodMay 31, 1990Rosalie VeilePurpose:Blood Samples can be stored frozen as a backup in case an LC
红细胞裂解的实验方法步骤
IntroductionPrior to using lymphoid tissue cell suspensions for flow cytometric analysis and/or for in vitro functional assays, it is recommended to r
Examination－of－a－Mammalian－Blood－Smear
Examination of a Mammalian Blood SmearThe distribution and appearance of the formed elements of blood can tell a great deal about the condition of the
Mouse－Compete－Blood－Counts
Materials: 250 µL of fresh mouse blood in plastic tubes containing EDTA. RBC lysis buffer (388 mM NH4Cl, 29.7 mM NaHCO3, 25 µM Na2EDTA)20.75g. NH4CL2.
Erythrocyte－Differentiation－Pathway
Stem cells in the bone marrow produce a variety of hematopoietic cell types from common progenitor cells under the influence of cytokines and growth f
Hemoglobins－Chaperone
The function of hemoglobin in oxygen transport by red blood cells requires precise assembly of a tetramer of two beta-subunits and two alpha-units. If
Blood－Smear:－Preparation－and－Staining
Blood Smear: Preparation and StainingReference:Davidson, I. and Henry J., Clinical Diagnosis by Laboratory Methods, I. Davidsohn and J. Henry, eds., W
Cell－Surface－Immunofluorescence－Staining－Protocol
实验概要A method of identifying and enumerating specific cell types in a heterogeneous population of cells by enhancing the specific staining of desired
SQ－Blood－DNA－Maxi－Protocol－for－410－ml－whole－blood
实验概要The E.Z.N.A.® SQ Blood DNA Kit is designed for isolating high molecular weight genomic DNA from fresh, frozen or anticoagulated whole blood. The
SQ－Blood－DNA－Midi－Protocol－for－500l3ml－whole－blood
实验概要The E.Z.N.A.® SQ Blood DNA Kit is designed for isolating high molecular weight genomic DNA from fresh, frozen or anticoagulated whole blood. The
MaxiYield－protocol－for－10－20－ml－Whole－Blood
实验概要The E.Z.N.A.® Blood DNA Maxi Kit is designed for isolation of genomic DNA from up to 25 ml of fresh, whole blood treated with any common anticoa
STANFLRED标准品
荧光标准固体荧光副基准，STAN-FL-RED可以用于对你的荧光系统做快速和简易的校正，稳定性和性能检测。STAN-FL-RED适合插入海洋光学的试管支架，例如CUV-ALL 4-Way。
血细胞分析报告内容
（一）数值报告 1. 红细胞相关参数： 至少报告红细胞计数（red blood cell count，RBC）、血红蛋白（hemoglobin，Hb）、血细胞比容（hematocrit，Hct）、平均红细胞体积（mean corpuscular volume，MCV）、平均红细胞血红蛋白含量
Different－types－of－human－cells
Human primary cells are cells taken from living human beings and cultured. These cells retain the differentiation of the original cells taken in
Application－Note:－Qdot®－Nanocrystal－Conjugates－in－Flow－Cytometry
实验概要Researchers today are trying to maximize the information that they get out of flow cytometry experiments by looking at more parameters in a sing
Oxidative－reactions－of－the－pentose－phosphate－pathway
One form of chemical energy used to drive biosynthetic reactions forward is the reducing power of the energy carrier NADPH. NADPH is essential to driv
SQ－Blood－Mini－Protocol－for－1ml－Clotted－Blood
实验概要This protocol is designed for isolating genomic DNA from 1ml Clotted Blood.主要试剂Regents to be supplied by user1. Proteinase K (20mg/ml)2. Isopropan
SQ－Blood－Mini－Protocol－for－50ul－Clotted－Blood
实验概要This protocol is designed for isolating genomic DNA from 0.5-1 million cultured cells. For larger or smaller amounts of starting cell numbers,
Plasma－and－Serum－Preparation
实验概要Serum is the liquid fraction of whole blood that is collected after the blood is allowed to clot. The clot is removed by centrifugation and the
吞噬细胞功能检测的临床应用
试验原理是将人巨噬细胞与鸡红细胞(chicken red blood cell，CRBC) 混合后孵育，通过计算巨噬细噬 CRBC 百分率和吞噬指数判断人巨噬细胞的 吞噬功能。通过观察 CRBC 消化程度，反映巨噬的消化功能。
线粒体肿胀检测方法
Estimating Mitochondrial Mass，可以用于线粒体肿胀的检测Accurate measurements of mitochondrial mass require a probe that will accumulate in mitochondria regardless
Lineage－Analysis－of－Blood
Materials:Capillary tubes1.5 mL Eppendorf microfuge tubes15 mL conical centrifuge tubes96-well V-bottom plates (Corning Costar 3894, from Fisher)Flow
SQ－Blood－DNA－Mini－Protocol－for－Buffy－Coat
实验概要The buffy coat fraction of whole blood is enriched with WBC, and usually gives at least 5-fold more DNA than the same volume of blood. To prep
关于红细胞寿命的基本介绍
红细胞寿命（red blood cell life span，RBCS）：生理状态下，红细胞的生成和破坏呈动态平衡，健康成年人RBCS平均115（70~140）天。病理状态下，无论何种机制介导的红细胞破坏增多（即溶血），均可导致RBCS缩短，红细胞破坏超过骨髓代偿增生时，即导致贫血。
Immunofluorescent－Staining－of－Mouse－and－Rat－Leukocytes
I. ProcedureHarvest cells from tissue, preparing a single cell suspension. Red blood cells may be removed by lysis or density gradient: Red blood cell
Culture－of－Peripheral－Blood－Lymphocytes－for－Chromosome－Analysis
实验概要Provide information about chromosomal abnormalities.实验原理The blood cell karyotyping method was developed to provide information about chromosomal