HistopathologicalApproachtoRatLiverTissue
ProcedureAfter deep ether anesthesia, dissect the rat’s liver (Wistar albino rats, 200 – 250 g) by cutting on the ventral side.Fix 2 – 3 mm. of the liver tissue in neutral buffered formalin (10% formaldehyde in Phosphate buffered saline) over night.After fixation, place the tissues in 70% isopropyl alcohol for 3 hours and then in each ascending strength (80%, 90%, 100% isopropyl alcohol) for 2 hours each. The amount ......阅读全文
Histopathological－Approach－to－Rat－Liver－Tissue
ProcedureAfter deep ether anesthesia, dissect the rat’s liver (Wistar albino rats, 200 – 250 g) by cutting on the ventral side.Fix 2 – 3 mm. of the li
Rat－Liver－Preparation
实验概要The procedure presented below describes a method for preparing rat liver.主要试剂1. Aluminum Foil2. Liquid Nitrogen3. Dry Ice4. Ph
Preparation－of－Rat－Liver－Cell－Cytosol
These protocols should yield enough cytosol and organelles for 1-200 MT/Organelle motility assays.Solutions and Reagents Freshly removed or flash fro
Preparation－of－Mitochondria－from－Rat－Liver
Preparation of Mitochondria from Rat LiverRat liver is an ideal source for functional intact mitochondria for a number of reasons. We use Sprague-Dawl
Preparing－Mitochondria－from－Rat－Liver
Liver is a convenient source for functional intact mitochondria for a number of reasons. Animal tissue is more readily homogenized than plant tissue b
Isolation－of－liver－lymphocyte
Isolation of liver lymphocyte Several lymphocyte subpopulations reside in the normal adult human liver. Liver lymphocytes mainly include a large n
GentleMACS/单细胞悬液制备文献集锦
种属 处理组织 文章名称 human
Isolation－of－liver－infiltrating－lymphocytes
1. Hepatic T cells were isolated from fresh liver tissue collected into primary cell system.2. In the second method, tissue was diced using sterile bl
Regulation－And－Function－Of－ChREBP－in－Liver
Liver is the major site for carbohydrate metabolism (glycolysis and glycogen synthesis) and triglyceride synthesis (lipogenesis). While insulin was lo
Primary－cultures－of－intrahepatic－bile－duct－epithelial－cells－isolated－and...
Primary cultures of intrahepatic bile duct epithelial cells isolated and cultured1. Liver was surgically removed and perfused via the hepatic vein.2.
RNA提取
RNA提取（主要内容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Procedures for Handing
Extraction－of－DNA－using－DNAzol®－Reagent
实验概要DNAzol® Reagent (Genomic DNA Isolation Reagent) is a complete and ready-to-use reagent for the isolation of genomic DNA from solid and liquid sa
Rat－Lung－Perfusion
实验概要The procedure presented below describes a method for perfusing rat lung.主要试剂Phosphate Buffered Saline主要设备1.Dissecting Microscope or Eye Magnifier2
Targeted－Gene－Replacement－in－Fungi－Using－a－SplitMarker－Approach
Targeted gene replacement is one of the primary strategies for functional characterization of fungal genes and several methods have been developed
TISSUE－CULTURE－ON－COVERSLIPS
I. Purpose:A. Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspected
PCR－from－Tissue
collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOHput in boiling H2 O for 30 sec (optimum may n
Tissue－Harvest－Protocol
TISSUES TO BE PROCURED(minimally and preferably within 5-8 hours after death):1. Brain2. Liver3. Muscle4. Skin5: Others as the specific case dictatesP
Tissue－Culture－Media
We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in a
PCR－from－Tissue
1.collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOH 2.put in boiling H2O for 30 sec (optimum
PCR－from－Tissue
collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOHput in boiling H2O for 30 sec (optimum may ne
Isolation－and－Culture－of－Human－Liver－Stem－Cells
Materials and Methods1. Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy liver tissue (
Thawing－－Incubating－Human－－Animal－Liver－Microsomes
实验概要BackgroundThe liver is the major organ for metabolism of endogenous substrates as well as exogenous drugs. There are several in vitro tools avai
Methods－for－the－Detection－of－DAminoAcid－Oxidase2
Results and DiscussionNavigationAbstractIntroductionMaterials and MethodsResults and DiscussionReferencesD-Amino-acid oxidase activity was detected in
Rat－Blood－Collection－Protocols
实验概要The procedure presented below describes a method for collecting rat blood.实验步骤Rat should be fully anesthetized (e.g., unresponsive to toe pinch).1
PCR－from－Plant－Tissue
PCR from Tissue Reference: Klimyuk et.al., 1993, Plant J. 3:493-494 Last updated: 1/27/00 By: Kay Schneitz collect piece of tissue (e.g., piec
Tissue－preparation－protocol－for－ChIP
实验概要This protocol describes how chromatin is prepared from tissue, which can subsequently be used for chromatin immunoprecipitation (ChIP). It is re
DNA－Extraction－from－Tissue
实验概要DNA extraction from tissue.主要试剂Extraction buffer100 mM Tris-HCl (pH 8.0) 100 mM EDTA (pH 8.0) 100 mM Na-Phosphate (pH 8.0) 1.5 M NaCl1% CTAB
Stock－solutions－for－tissue－culture
The kitchen makes Tris, TD, Tryp/TD, PBS, and VE.Tris is a fairly complex, Tris-buffered physiological saline solution. It is used to wash cells and i
Dissociation－of－spleen－and－hemopoietic－tissue
You need to buy glass slides with frosted, sandblasted ends (Fisher Scientific, Catalog N. 12-552). Frosting by painting (e.g.Superfrost) should not
PCR－from－Plant－Tissue
1.protocol(1)collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOH(2)put in boiling H2Ofor 30 sec