CriticalAppraisaloftheMTTAssayinthePresenceofRottlerin3
LDH assay LDH assay was performed in culture medium of untreated confluent cells by using a commercial kit (Sclavo Diagnostics, Siena) based on the transformation of pyruvate to lactate by LDH, at pH 7.5, in the presence of NADH coenzyme. The transformation of NADH to NAD+ is accompanied by a decrease in absorbance (A) at 340 nm, which correlates with the LDH activity. The change of absorbance, in th......阅读全文
Critical－Appraisal－of－the－MTT－Assay－in－the－Presence－of－Rottlerin－3
LDH assay LDH assay was performed in culture medium of untreated confluent cells by using a commercial kit (Sclavo Diagnostics, Siena) based on the
Critical－Appraisal－of－the－MTT－Assay－in－the－Presence－of－Rottlerin－4
Table 1 MTT reduction normalized to cell numberTime (h)MTT/cell numberR5R2011.231.5421.201.5341.201.70151.252.24241.583.49The MTT (% control)/cell num
Critical－Appraisal－of－the－MTT－Assay－in－the－Presence－of－Rottlerin－5
Because an immediate sign of mitochondrial uncoupling is the drop in cell phosphorylation potential, we evaluated the Rottlerin uncoupling effect by d
Critical－Appraisal－of－the－MTT－Assay－in－the－Presence－of－Rottlerin－1
Rottlerin is a natural product isolated from Mallotus philippinensis. This polyphenolic compound, originally described as a selective inhibitor of PKC
Critical－Appraisal－of－the－MTT－Assay－in－the－Presence－of－Rottlerin－2
Materials and methodsMaterials All chemicals and materials for cell culture (unless otherwise indicated) were obtained from Sigma (Milan, Italy). La
Critical－Appraisal－of－the－MTT－Assay－in－the－Presence－of－Rottlerin－6
Our experience indicates that it may not be sufficient to change the medium containing Rottlerin and to wash the cells before adding MTT to avoid a po
MTT－Assay
This procedure is for cells in 96 well plates, if larger plates are used then adjust volumes accordingly.1 Make a solution of 5mg/ml MTT dissolved in
MTT－Cell－Proliferation－Assay
MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, first described by Mosmann in 1983, is based on the ability of a mitochondri
Cytokine－Bioassays
Cytokine BioassaysIntroductionBiological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary cel
Neutralizing－Bioassay－Protocols
Neutralizing Bioassay ProtocolsIntroductionAntibodies that block binding of cytokines to their specific receptors and neutralize their effects are cri
Hanging－drop－aggregation－assay
DescriptionThis assay is used for the aggregation property of cancer cells. It is a very critical parameter for measurement of cell metastasis. Factor
TCell－Activation－Using－mAb－to－CD3
IntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequ
Culture－of－rheumatoid－arthritis－synoviocytes－and－collection－of－synovial－...
Culture of rheumatoid arthritis synoviocytes and collection of synovial fluids Culture of rheumatoid arthritis synoviocytes and collection of syn
Pectinase－assay
Pectinases are actually a mixture of enzymes, which, along with others such as cellulase, are widely used in the fruit juice industry where they are
DGK－Assay
Buffers: - 2X buffer 10 ml 0.5 M imidazol, pH 6.6 0.21 g LiCl 1.25 ml 1 M MgCl2 1.0 ml 0.1 M EGTA, pH 6.6 --> Bring volume up to 50 ml with distille
Phosphate－Assay
1. Make standards using sodium phosphate at the following uM concentrations: 0, 2, 5, 7, 10, 20, 40, 60, and 80. Use the screw top glass tubes.2. Dry
Polygalacturonase－assay
This enzyme is famous for being involved in the development of the GMO tomatoes (more information from the link at the foot of this page). The cells o
Bradford－Assay
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue
Bradford－Assay
Bradford AssayThe bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie B
Chemotaxis－Assay
PurposeThe purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cel
Motility－Assay
DescriptionVarious phenotypic characteristics are requiredfor a cancer cell to successfully complete the metastaticcascade. Among these, acquisition o
TUNEL－assay
PROTOCOL:•Deparaffinize and rehydrate slides:3 x 3´ Xylene3 x 2´ 100% ethanol1 x 2´ 95%, 80%, 70% ethanol (each)1 x 5´ 1x PBS•Microwave antigen retrie
Protease－assay
In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to softe
Aspartate－Assay
实验概要The Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted
Protease－assay
实验概要 In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in
凝胶延长分析二型脂肪酸合成的方法
Gel-elongation assay for type II fatty acid synthesisSrinivas KodaliAndrew GalgociSheo Singh Dr.Jun Wang Dr., jun_wang2@merck.com, Merck Research Labo
四唑盐（MTT）比色实验——MTT法
实验方法原理活细胞的线粒体中的琥珀脱氢酶能使外源性的MTT还原为难溶性的蓝紫色结晶物并沉积在细胞中，而死细胞无此功能。二甲基亚砜能溶解细胞中的紫色结晶物，用酶联免疫检测仪在490 nm 波长处测定其光吸收值，可间接反映细胞数量。在一定细胞数范围内，MTT结晶物形成的量与细胞数成正比。 实验材料细胞
信号传导
Cytokine Bioassays (eBioscience)Biological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary c
信号传导
Cytokine Bioassays (eBioscience)Biological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary c
Assay－of－Phospholipase－A－Activity
Phospholipases of the A type constitute a large family of esterases that catalyze the hydrolysis of the fatty acid ester bonds in phospholipids a