PlateletPreparation
OUTLINEIn order to avoid platelet activation all manipulations must be performed as quickly and as acurate as possible.Work on ice if possible! This protocol is based on differential centrifugation that allows to separate platelets from blood cells. Also you can use Robinson procedure (consult the HANDBOOK OF FLOW CYTOMETRY METHODS) PROTOCOLBleed animals via the plexus retroorbitalis or the tail vein under ......阅读全文
viral－immunity－－pathogenesis－group
OUTLINEWe consider manual counts as less quick but more precize than authomatic (electronic) since in the latter case the counts are based on the size
Platelet－Preparation
OUTLINEIn order to avoid platelet activation all manipulations must be performed as quickly and as acurate as possible.Work on ice if possible! This p
ELISA－with－Platelet
OUTLINEThis modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies o
CMFDA－Labeling－of－Platelet
OUTLINECMFDA (5-chloromethylfluorescein diacetate) is a lipophilic tracer that has an enormous advantage over ordinary tracers (e.g. FITC) because it
Western－Blot－with－Platelet－Protein
OUTLINEWestern blot is a wide used technique to identify a target protein/s for the certain antibody.PROTOCOLPrepare platelets.Lyse washed platelets (
Platelet－Amyloid－Precursor－Protein－Pathway
The amyloid -beta peptide (Ab), a proteolytic fragment of amyloid precursor protein (APP), is the major componenet of senile plaques, the hallmark of
中法NSFC—CNRS合作交流项目获批名单公布
国家自然科学基金委员会（NSFC）与法国国家科学研究中心（CNRS）在2011年度共同资助合作交流项目，经双方各自评审和协商，共有以下10个项目获得批准： 序号申请项目名称 中方申请人依托单位 法方申请人依托单位1扩展孤立子方程的显示解及其在流体力学中的应用 林润亮 清华大
Plateletassociated－Ig－(PAIg)－Elution
OUTLINEPlatelet-associated Ig (PAIg) could be eluted from the platelets. As a result of this procedure platelets get destroyed and the eluted Ig is fo
Eph－Kinases－and－ephrins－support－platelet－aggregation
Eph kinases are a family of receptor tyrosine kinases with an extracellular domain that binds their ligand, the ephrins, and an intracellular kinase d
Liposome－Preparation
Liposome PreparationOBJECTIVE:Method for incorporating proteins into liposomes for liposome swelling assay or as an alternative antigen presentation m
SMEAR－PREPARATION
The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria
Preparation－of－tubulin
Although many protocols for tubulin preparation are available, the procedure described below is the simplest and highest yielding preparation I have d
CAM－preparation
8 eggs per day, day 7- day 13 cut CAM, wash in precooled PBS,in 10 ml WASH 1 (PBS, 5 mM EDTA, COMPLETE) on icecut in pieces in petri dish on icecentri
Template－Preparation
Template PreparationThe quality of sequencing results is directlyrelated to the quality of the template. ABI recommends a minialkaline-lysis/PEG preci
Dephosphorylation－...
实验概要The method provides a protocol for removal of phosphate groups from proteins, before or after blotting. To demonstrate the specificity of an ant
Aspirin－Blocks－Signaling－Pathway－Involved－in－Platelet－Activation
Activation of the protease-activated GPCRs in platelets contributes to platelet activation in clotting. The protease-activated receptors PAR1 and PAR4
Multiple－studies－with－a－single－experiment:－The－Power－of－...（七）
SPS TMT Method Development- What is New on Lumos Multiplexing On the Benchtop Orbitrap System (Q Exactive Series) •Take advantage of segmented quadrup
蛋白质提取和纯化
蛋白质提取和纯化（主要内容如下）Protein Extraction Protein PurificationProtein PrecipitationColumn PreparatioinQ & A Posted in the Method ForumProtein ExtractionWhole
基金委公布与法国国家科研署合作研究项目初审结果
2011年国家自然科学基金委员会(NSFC)与法国国家科研署(ANR)在材料与工程和信息与通信领域(含纳米科技)共同资助合作研究项目。经公开征集，根据国家自然科学基金委员会有关规定并与法方核对申请项目清单，共有如下58项申请通过初审：编号申请项目名称申请人/依托单位法方合作者/依托单位1热成形超
Dephosphorylation－of－DNA
De-phosphorylation of DNAThe preferred method of de-phosphorylation uses the buffer system described by Pharmacia for most of their restriction endonu
Rat－Liver－Preparation
实验概要The procedure presented below describes a method for preparing rat liver.主要试剂1. Aluminum Foil2. Liquid Nitrogen3. Dry Ice4. Ph
Preparation－of－human－platelets
Preparation of human platelets 1. Human blood was taken from drug-free volunteers on the day of the experiment using acidic citrate dextrose
HELPER－PHAGE－PREPARATION
HELPER PHAGE PREPARATION1. Grow an overnight of NM522 in NZCYM medium.2. Dilute overnight 1:100 and grow to an A600 = 0.3 (@2.5 x 108 cells/ml).3. Inf
Preparation－of－Polyacrylamide－Gels
1. Prepare 20X TBE as:216 g Tris Base110 g Boric Acid80 mL 500 mM EDTA, pH 8.0700 mL ddH2OMix. Bring volume to 1 L. Autoclave.2. Prepare Acrylamide so
Lambda－DNA－Preparation
Lambda DNA PreparationThis is a plate method that gives very good yield for cloning. I have combined the Promega and Maniatis protocols.Solutions T-TY
Plasma－and－Serum－Preparation
实验概要Serum is the liquid fraction of whole blood that is collected after the blood is allowed to clot. The clot is removed by centrifugation and the
Preparation－of－Mouse－Neutrophils
实验概要Preparation of Mouse Neutrophils实验步骤Mice：8-16 weeks old malePrewarm buffer to room temperatureBuffer A : Ca2 /Mg2 ‐free Hank’s buffered saline so
Competent－Cell－Preparation
实验概要Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. These cells readily incorporate f
Preparation－of－Agar－plates
Prepare media and add 1.5 agar before autoclaving it (15g per liter). After autoclavation, cool the media in a 55 degree waterbath. Do not allow the s
Metaphase－chromosome－preparation
Materials: RPMI 1640 medium fetal calf serum (FCS), 20% Colcemid (e.g. Boehringer Mannheim cell biology reagents, Best.-Nr. 295892) cell cuture flask