Protocolforevaluatingvascularpermeabilityinwildtype
1) Female Tiel-PR Tg mice and wildtype control litter-mates aged 6-9 wks wereovariectimized and allowed to recover for 7-10 days.Making estrogen and progesterone stock solutions:For estrogen (17 beta-estradiol from Sigma), weigh out 10 mg and add to 1 ml 200-proofEtOH. This will yield a lOmg/ml solution. Mix by vortexing. Perform a 1:100 dilution byadding 5 μl of the lOmg/ml solution to 495 μl EtOH. This will leave y......阅读全文
Protocol－for－evaluating－vascular－permeability－in－wildtype
1) Female Tiel-PR Tg mice and wildtype control litter-mates aged 6-9 wks wereovariectimized and allowed to recover for 7-10 days.Making estrogen and p
Ion－Channels－and－Their－Functional－Role－in－Vascular－Endothelium
Endothelial cells (EC) form a multifunctional signal-transducing surface that performs different tasks dependent on its localization in the vessel tre
血管表皮生长因子（VEGF）与肿瘤生长
随着医疗水平的不断提高，肿瘤的内科治疗也有了放疗、化疗、靶向药物治疗。其中靶向治疗中又以“抗血管生成”为近来研究热点。肿瘤的生长、转移需要丰富的血管为其提供足够的氧气和营养物质,肿瘤组织可分泌多种促血管生成物质，并通过多条血管生成信号通路的转导及通路间的相互作用诱导、调控血管的生成。新血管的形成(a
Isolation－and－Culture－of－human－vascular－smooth－muscle－cells
1. Human vascular smooth muscle cells (SMCs) were isolated from human saphenous vein.2. Small moistened pieces of tissue (6 mm2) were placed intima
Measurement－of－primary－endothelial－cell－permeability－to－fluxes－of－dextran..
Measurement of primary endothelial cell permeability to fluxes of dextran or albuminThe fluxes of albumin or dextran across vascular endothelial cell
血管内皮生长因子的基本介绍
血管内皮生长因子（vascular endothelial growth factor，VEGF），又称血管通透因子（vascular permeability factor，VPF）是一种高度特异性的促血管内皮细胞生长因子，具有促进血管通透性增加、细胞外基质变性、血管内皮细胞迁移、增殖和血管形
血管内皮生长因子的结构和功能特点
血管内皮生长因子（vascular endothelial growth factor，VEGF），又称血管通透因子（vascular permeability factor，VPF）是一种高度特异性的促血管内皮细胞生长因子，具有促进血管通透性增加、细胞外基质变性、血管内皮细胞迁移、增殖和血管形成等
血管内皮生长因子的概述
血管内皮生长因子（英文：vascular endothelial growth factor，简称：VEGF），早期亦称作血管通透因子（英文：vascular permeability factor，简称：VPF），是血管内皮细胞特异性的肝素结合生长因子。 它具有促进血管通透性增加、细胞外基质
Isolation－of－stromal－vascular－cells－from－human－adipose－tissue
Stromal stem cells proliferate in vitro and may be differentiated along several lineages. The scarcity of stromal stem cells in tissues and the la
SPSpermeability－kit渗透率检测仪
渗透率检测的意义渗透率是薄膜类材料的重要特性，精确测量薄膜、纸张等的水分子渗透率对于评估其作为包装材料在不同水蒸汽分压环境下隔绝水分的功能有着重要的意义。 渗透率检测方法一种很常见的方法是将薄膜（纸张）置于一边是相对湿度很高，另一边是干燥流动气体的环境中。水分子会透过薄膜（纸张）被干燥气体吹至NIR
Physcomitrella－patens－:－A－NonVascular－Plant－for－Recombinant－Protein...
The moss Physcomitrella patens is a long-standing model for studying plant development, growth and cell differentiation in particular. Interest in
Cells－and－Molecules－involved－in－local－acute－inflammatory－response
Inflammation has several distinct components, including the localized response at the site of tissue injury or infection. Tissue injury stimulates the
Immunoprecipitation－Protocol
实验概要Immunoprecipitation is a procedure by which proteins or peptides that react specifically with an antibody are removed from solution and examined
ELISPOT－Protocol
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on t
ELISPOT－Protocol
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on t
ELISA－protocol
ELISA protocol：1.取5－10ul BMMY表达上清用0.05M NaHCO3稀释到100ul铺ELISA板，37度或室温振荡大于1小时。注意一定要做一个GS115空菌株表达上清作为阴性对照，最好还找一个带有histag的蛋白作为阳性对照。2.TPBS洗板3次，方法：倒掉铺板液，倒置于
Immunoblot－Protocol
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
RNAi－protocol
siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western
NAi－protocol
siRNA protocolsOur current strategy with siRNA is to synthesis relatively small amounts enzymatically and use these to test for efficiency by western
ELISPOT－protocol
实验概要The procedure below is a general guideline procedure for ELISPOT. Abcam ELISPOT kits have been designed for detection of various cytokines and g
PCR－protocol
PCR reactionProtocol for 50µl reaction - adjust amounts if necessary, for a 20µl reaction use the same volumes of primer and dNTP-mix, but adjust the
RLGS－protocol
A. Preparation of DNA SolutionIn the case of rice, for example This method may be appllicable for many grass species and some other plants.
免疫细胞化学
Introduction to Immunocytochemistry (House Ear Institute)A brief overview of common available methods. BrDU Immunocytochemistry using peroxidase and
VEGFC基因编码的功能和结构描述
该基因编码的蛋白是血小板衍生生长因子/血管内皮生长因子（pdgf/vegf）家族的成员。编码蛋白促进血管生成和内皮细胞生长，并能影响血管的通透性。前蛋白进一步被切割成一个完全加工的形式，可以结合和激活vegfr-2和vegfr-3受体。The protein encoded by this gene
Microarray－Hybridization－Protocol
Introduction:One microarray set consists of 7 nylon membranes with 2.5 x 7.5 cm dimension. 2304 genes were spotted onto nylon membranes (Schleicher an
Nuclear－Extraction－Protocol
实验概要The procedure presented below describes a method for extracting nuclear from several cell lines of human origin.主要试剂Hypotonic Buffer Solution20 mM
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
Yale－Immunofluorescence－Protocol
实验概要We provide a protocol for fixation, immunostaining, and imaging in 384-well Plates.主要试剂Reagents1. 384-well view plates (Aurora)2. HUVEC (pooled, L
Bacteria－Culture－Protocol
Bacteria Culture ProtocolBy 徐晓政1、TBS Medium Preparation:Prepare 1L of TBS medium contains:Tryptone 12gYeast extract 24gNaCl 5gSodium Succinate 5gGlyce
Intracellular－Staining－Protocol
1. Fix cells- Add16% formaldehyde directly into culture medium to obtain a final concentration of 1.5% formaldehyde.2. Incubate in fixative for 10 min