Detectionofapoptoticprocessinsituusingimmunocytochemical2
B) TUNEL in situ procedureB.2.1 Materialsproteinase K (pK) (A2), H2O2 , TdT buffer (A1), TdT enzyme (A2), biotinylated dUTP (A2), TB buffer (A1), serum albumin (BSA) (A2), PBS, Extra-avidin Peroxidase (A2), AEC solution (A1), DNAse buffer (A1). B.2.2. Methodology1. Tissue sections are dewaxed and rehydrate by heating at 60°C. 2. Wash in xylene and rehydrate through a graded series of ethanol and deioni......阅读全文
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