ChIPusingplantsamples–Arabidopsis
实验概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). The exact chromatin concentration should be determined before starting the X-ChIP assay. View our cross-linking chromatin immunoprecipitation (X-ChIP) protocol, which should be used after the chromatin preparation detailed below.实验原理Eukaryotic chromatin is a co......阅读全文
ChIP－using－plant－samples－–－Arabidopsis
实验概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). T
ChIP－using－plant－samples
实验概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin immunoprecipitati
Studying－Arabidopsis－Envelope－Protein－Localization－and－Topology－Using－...
Chloroplasts are metabolically important organelles that perform many essential functions within plant cells. The chloroplasts can be subdivided i
GenomeWide－Identification－of－Transcription－FactorBinding－Sites－in...
Genome-Wide Identification of Transcription Factor-Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip)
Extraction－of－DNA－From－Plants－Using－Plant－DNAzol®－Reagent
实验概要Plant DNAzol® is an extra-strength-DNAzol® reagent (patent pending) specifically formulated for the isolation of genomic DNA from plants. The Plan
Detection－of－Viruses－in－Infected－Plant－Extracts－using－ImmunocapturePCR
1) Immunocapture stageCoating buffer: 15 mM Na2CO3; 35 mM NaHCO3, and 3 mM NaN3, per liter (pH 9.6).Extraction buffer: (20 mM Tris-HCL (pH 8.0), 138
Detection－of－Viruses－in－Infected－Plant－Extracts－using－ImmunocapturePCR
1) Immunocapture stageCoating buffer: 15 mM Na2CO3; 35 mM NaHCO3, and 3 mM NaN3, per liter (pH 9.6).Extraction buffer: (20 mM Tris-HCL (pH 8.0), 138
Verification－of－In－...
实验概要Environmental stresses often trigger rapid generation of reactive oxygen species (ROS) in plants. Excessive amount of ROS can cause damage to
DoseResponse－to－and－Systemic－Movement－of－Dexamethasone－in－the－GVGInducible
DoseResponse to and Systemic Movement of Dexamethasone in the GVG-Inducible Transgene System in ArabidopsisConstruction of transgenic plants is centra
Global－Expression－Profiling－of－RNA－from－Laser－Microdissected－Cells－at－...
Global expression profiling of RNA isolated from laser microdissected cells allows one to profile a specific set of cells allowing for enhanced se
悬挂－Hanging－Samples
悬挂 Hanging Samples大组织透明化样品可以通过悬挂的方式放置在样品载物台上，样品最大尺寸是1cm*1cm*1cm。适合样品：水溶性试剂透明化的小鼠胚胎、脑、肺、肝脏、肾脏、胰腺、卵巢等。上样方式：平台配有不同形式的样品粘托和样品托盘，可以将样品通过粘贴悬挂或者托挂的方式放置在载物台上，
包埋－Embedded－Samples
包埋 Embedded Samples可以将部分样品包埋在1-4%浓度的低熔点琼脂糖中，不同浓度的琼脂糖其稳定性不同，对活体样品的影响也不同。适合样品：斑马鱼、果蝇、线虫、拟南介等荧光标记的模式生物。包埋尺寸：平台配有不同规格的毛细管和1ml注射器，样品大小0.23mm-3.06mm，介于毛细管和注
Tissue－preparation－protocol－for－ChIP
实验概要This protocol describes how chromatin is prepared from tissue, which can subsequently be used for chromatin immunoprecipitation (ChIP). It is re
In－Planta－Transformation－of－Arabidopsis
实验概要 A breakthrough in Arabidopsis research was the invention ofthe vacuum-infiltration procedure, a simple and reliable methodof obtaining
Transformation－Protocol－for－Arabidopsis
Transformation Protocol for Arabidopsis – AbbreviatedGerminate seed in pots↓ 4 weeksStreak bacteria onto YM/MinA↓ 2-3 days 28°CSpray/dip bacterial sus
Arabidopsis－gDNA－isolation
This is a simple and fast protocol for the extraction of genomic DNA from Arabidopsis thaliana that works fine in PCR for simple amplicons. We only us
Quantitating－RNA
RNA quantitation is an important and necessary step prior to most RNA analysis methods. Here we discuss three common methods used to quantitate RNA an
Use－of－Nonaqueous－Fractionation－and－Metabolomics－to－Study－Chloroplast－...
Use of Non-aqueous Fractionation and Metabolomics to Study Chloroplast Function in ArabidopsisChloroplasts are the chemical factories of plant cells b
Heterogeneity－of－SingleCell－Gene－Expression－Across－Phenotypically（二）
Figure 2: Verification of C1 Single-Cell mRNA-Seq data quality. a)ERCC RNA Spike-In Control Mix 1 was applied to a C1 IFC at a total transcript in
Estimation－of－Fat－in－Milk－Samples
实验概要Milk fat measurement is a common task in the dairy industry, because milk fat content is one factor that determines milk price and is necessar
围封－Enclosed－Samples
围封 Enclosed Samples由于高浓度的琼脂糖浓度会影响部分活体样本的发育和状态，琼脂糖浓度太低又会影响样品的稳定性，此时，我们可以选择将样品围封在FEP管中。适合样品：折射率和FEP、琼脂糖相似的样品，如斑马鱼、果蝇、线虫、拟南介等。样品大小：平台配有不同规格的FEP管，具体围封方式类
Estimation－of－Fat－in－Milk－Samples
IntroductionMilk fat measurement is a common task in the dairy industry, because milk fat content is one factor that determines milk price and is nece
Preparation－of－Stroma,－Thylakoid－Membrane,－and－Lumen－Fractions－from－...
Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic AnalysisFor many studies regarding
Total－Protein－Extraction－with－TCAAcetone
We describe a procedure allowing extraction of total proteins that performs efficiently with a large variety of plant tissues, based on simultaneo
Cell－Suspension－Culture－of－Arabidopsis
实验概要Cell Suspension Culture of Arabidopsis 主要试剂10% (v/v) Household BleachCallus Induction Medium Gamborg's B5 Basal Medium 0.5 g/liter M
Simplified－Arabidopsis－Transformation－Protocol
(Brief version for those who are familiar with the method)Steve Clough and Andrew Bent, University of Illinois at Urbana-Champaign.Our present proto
Arabidopsis－RNA－extraction－protocol
1-2 g fresh material, freezer-dried, ground with 0.2g sand (if necessary), and then homogenized with 10ml RNA extraction buffer (see below).Spin at 8,
Simplified－Arabidopsis－Transformation－Protocol
实验概要Our present protocol (Clough and Bent, 1998; modified from Bechtold et al. 1993) is extremely simple. We have found that the MS salts, hormone
Arabidopsis－RNA－extraction－protocol
1-2 g fresh material, freezer-dried, ground with 0.2g sand (if necessary), and then homogenized with 10ml RNA extraction buffer (see below). Spin
ChIPChip－E.－coli
AbstractChIP-Chip stands for Chromatin Immunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genome-wide bin