CultureofHumanEmbryonicStemCells(hESC)
All cell lines are initially grown according to the supplier's protocols but we are adapting them to one simple protocol outlined below:6-well plates (Falcon Cat #353046) are coated for 20 to 60 minutes at room temperature with 0.1% gelatin (Sigma Cat #G1890) in dH2O.Mouse embryonic fibroblasts (CF1 strain), cultured in MEF medium, are mitotically inactivated by treatment with 10μg/ml mitomycin C (Roche Cat ......阅读全文
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells2
Slide Coating13. Just before use, dilute the poly-D-lysine stock solution 1:50 in H2O. Add 0.2 mL of diluted poly-D-lysine to each well of an eight-we
Primary－Culture－of－Pulmonary－Arterial－Smooth－Muscle－Cells
Primary Culture of Pulmonary Arterial Smooth Muscle Cells 1.Primary cultures of Pulmonary Arterial Smooth Muscle Cells (PASMCs) were isolated from hum
Isolation－and－culture－of－rat－coronary－microvascular－endothelial－cells
CMVE were isolated from Wistar rat hearts by digestion of primary cell isolation kit.1. Hearts mounted on a Langendorff apparatus were perfused at 3
Isolating－Xenograft－Tumor－Cells－for－Tissue－Culture－or－Transplantation
Isolation of T4 cells from xenograft tumors Put mouse downWash skin by dipping mice into ETOHRemove tumor and put into 5ml PBS containing Fungizone an
胚胎干细胞和成体干细胞标志物
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
Isolation－of－human－primary－ovarian－surface－epithelial－cells
1. Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institutional Review (References)
Mesenchymal－progenitor－cells－derived－from－human－muscle
Harvesting traumatized muscle derived mesenchymal progenitor cells (MPCs)1. The muscle tissue was dissected without contaminating by granulation, adip
Isolation－of－human－primary－gastric－mucosa－epithelial－cells
1. A piece of gastric mucosa (∼3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2. The patients were proven to be no H.
Fluidigm公司微液流芯片在单细胞研究中的应用（三）
The next playersBeyond cell imaging and gene expression, other applications of single stem cell analysis are hardly thick on the ground. No one ca
New－Brunswick－S41i－CO2－恒温摇床进行间充质干细胞扩增（二）
Stem cell differentiation assaysAdMSCs were harvested from both shake and spinner flasks into 50 ml tubes. Once the microcarrier beads settled to the
胚胎干细胞培养
Media and Solution required for ES Cell Culture (Bowtell Lab) Routine Culturing of ES Cells (Bowtell Lab) Routine Splitting and freezing of cells (
可靠的CCCadvanced－FN1无异源耗材支持人间充质干细...（一）
可靠的CCCadvanced FN1无异源耗材支持人间充质干细胞扩增表达Reliable and Robust Animal-Component-Free hMSC-BM Expansion on Ready-to-Use Eppendorf CCCadvanced™ FN1 Motifs Surf
精原干细胞能转化成卵细胞已经成为可能
上海交通大学医学院冯立新研究组新近研究发现精原干细胞可以被诱导形成卵细胞。在正常发育过程中，由上胚层细胞来源的原始生殖祖细胞(primordialgermcells,PGCs)是精原干细胞和卵母细胞的共同前体细胞。以往的研究发现在一定的培养条件下，精原干细胞可被诱导成具有多能性的干细胞。而另一研
New－Brunswick－S41i－CO2－恒温摇床进行间充质干细胞扩增（一）
A Novel Method for the Expansion of Mesenchymal Stem Cells using an Eppendorf New Brunswick™ S41i CO2 Incubator ShakerKhandaker Siddiquee and Ma Sha,
可靠的CCCadvanced－FN1无异源耗材支持人间充质干细...（四）
Figure 5: hMSC proliferation rate during long-term expansion in different animal-component-free culture systemshMSC expansion on the FN1 motifs surface
MED64应用手册：人类干细胞来源的心肌细胞应用
关键词：人类干细胞来源的心肌细胞 Human stem cell-derived cardiomyocytes (hSC-CMs) 搏动频率 beat frequency; 场电位时程 field potential duration (FPD)慢性药物作用 chronotropic drug ef
Live－imaging－with－Drosophila－tissue－culture－cells2
Materials & ReagentsDrosophila Schneider S2 cellsSchneiders Medium (GIBCO/Invitrogen), 10% fetal calf serum, Antibiotics (Sigma A5955)Depression slide
Live－imaging－with－Drosophila－tissue－culture－cells1
IntroductionLive imaging provides an important complementation to the "snapshot" view obtained in fixed tissue by immunofluorescence. It allows follow
CO2恒温摇床解决人胚肾－293－(HEK293)－细胞结团问题（一）
人胚肾 293 (HEK293) 细胞在重组蛋白表达中是最常见的宿主细胞。 这类细胞能够表达大量的膜蛋白，如 G 蛋白偶联受体 (GPCR) ，是无法在最常见的生物制药生产宿主，如：中国仓鼠卵巢 (CHO) 细胞中作表达。 HEK293 虽然是蛋白表达的极好宿主，然而 HEK293 细胞
可靠的CCCadvanced－FN1无异源耗材支持人间充质干细...（二）
Materials and MethodsShort-term cell growth evaluationLonza™ Poietics™ human mesenchymal stem cells (hMSC-BM, PT-2501, Lonza) derived from normal ad
2011第四届再生医学和干细胞大会日程安排
日 期 时 间 会 议 内 容 11月10日 08:30-17:00 会议注册 11月11日 08:30 集体照 08:30-12:00 开幕式和主题论坛 12:00-13:00 午餐
使用CCCadvanced™FN1无异源耗材培养人多能干细胞（二）
Materials and MethodsCell culture conditions and surface transitionCryopreserved hiPSCs (SC102A-1, SBI™, USA) were initially thawed and pre-cultivat
早期胚胎发育中的单胚胎细胞基因表达（二）
“We picked 42 genes to validate on the BioMark system,” Dr. Yao said. “We picked them to represent different functional categories.”“We used the F
细胞组织消化常用的几种酶的选择
直接从生物体获取的组织，一般需要将其消化成单个细胞才能进行体外培养。这种直接从离体组织获得的细胞，更接近于生物体内的生活状态，且生物性状尚未发生很大改变，因此在药物筛选、细胞移植、类器官培养、肿瘤研究等众多领域备受欢迎。但组织消化过程中常遇到多种问题，例如消化不完全、细胞死亡率高等。如何克服这些问题
Detection－of－MicroRNA－Heterogeneity－in－Single－Cells－Using－an－Automated
Introduction MicroRNA (miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate gene expression by both disrupting messenger RNA (mRNA
STEM－CELLS：增压干细胞创建新疗法
近日，发表在国际期刊《Stem Cells》上的一项研究指出，阿德莱德大学的研究人员发现了一种培养干细胞的新方法，该方法可使细胞生长的更快，更强壮。 Kisha Sivanathan博士说这在干细胞上是一个激动人心的突破性研究。“成人间充质干细胞可以从体内许多组织包括骨髓中获得，这种干细胞吸引
Sleeping－Beauty－transposon－mutagenesis－in－rat－spermatogonial－stem－cells
Sleeping Beauty transposon mutagenesis in rat spermatogonial stem cellsZoltán Ivics,1, 2 Zsuzsanna Izsvák,1, 2 Gerardo Medrano,3, 4 Karen M Chapman3,
Isolation－and－Differentiation－of－AmnionDerived－Stem－Cells－(ADSCs)
ADSC isolation, culture, and cloningRat amnion membrane is mechanically separated from the chorion of embryonic day 18.5 Sprague–Dawley rat embryosThe
《Stem－Cells》：胚胎干细胞长成软骨
美国莱斯大学的生物医学工程师发明了一项能够利用人类干细胞生长出软骨的新技术。这种方法能够用于培养膝盖、下巴、臀部和其他关节的软骨替换。 由于自然的软骨组织是不能够自我痊愈的，所以研究人员长期以来都在寻找培养替代的软骨的方法。培养的这种软骨可用于修复软骨损伤。这项研究为利用胚胎干细胞制造类软骨细胞提
Isolation－and－longterm－cultivation－of－Human－Corneal－Endothelial－Cells
Coating of the Culture Dish SurfacesCulture dishes were incubated with a film of a 1:1 mixture of laminin (10 μg/ml and chondroitin sulfate (10 μg