DetectionofMicroRNAHeterogeneityinSingleCellsUsinganAutomated
Introduction MicroRNA (miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate gene expression by both disrupting messenger RNA (mRNA) stability and inhibiting mRNA translation. The expression of miRNA species in cellular populations is thought to drive downstream gene expression and protein functionality. Our goal was to determine the variability in miRNA expre......阅读全文
Detection－of－MicroRNA－Heterogeneity－in－Single－Cells－Using－an－Automated
Introduction MicroRNA (miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate gene expression by both disrupting messenger RNA (mRNA
Heterogeneity－of－SingleCell－Gene－Expression－Across－Phenotypically（一）
Introduction Multi-cellular populations are fundamentally driven by the collective properties of individual cells. However, our understanding of ge
自动化的微流控芯片系统在单细胞中检测MicroRNA的异质性3
结论· 我们在C1TM单细胞自动制备系统开发了一种简洁的实验方案，能以最少的手工操作，在不到24小时内，平行处理高达96个单细胞，对其miRNA表达谱进行分析。· C1 miRNA STA实验方案使用了Life Technologies为miRNA优化过的试剂。特别的，Megaplex™ RT及
Dual－and－TripleCo...－（一）
实验概要Cytokine ELISPOT has become a powerful routine tool for the analysis of disease- as well as vaccine-induced T-cell responses. The method is limi
Heterogeneity－of－SingleCell－Gene－Expression－Across－Phenotypically（二）
Figure 2: Verification of C1 Single-Cell mRNA-Seq data quality. a)ERCC RNA Spike-In Control Mix 1 was applied to a C1 IFC at a total transcript in
Detection－of－apoptotic－process－in－situ－using－immunocytochemical
1. INTRODUCTION Apoptosis was observed from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pathological
Transfection－of－Mammalian－Cells－Using－Lipofectamine
Materials: LipofectamineBasal Medium containing 10% fetal bovine serum, 1% glutamine, 1% aaBasal Medium containing 1% glutamineBasal Medium cont
Detection－of－BrdU－Incorporation－in－DNA－Synthesizing－Cells
Detection of BrdU Incorporation in DNA Synthesizing Cells NOTE: Bromodeoxyuridine is a known carcinogen. Propidium iodine (PI) is known to be toxic an
ELISPOT－(Enzymelinked－ImmunoSPOT)－实验方法步骤1
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, bu
ASENSITIVE－METHOD－FOR－DETECTION－OF－APOPTOSIS－BY－SINGLE－LASER－FLOW－CYTOMETRY
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-
SingleVirus－Tracking－in－Live－Cells
Single-Virus Tracking in Live CellsMichael J. Rust, Melike Lakadamyali, Boerries Brandenburg and Xiaowei Zhuang INTRODUCTIONReal-time, live-cell imagi
Detection－of－apoptotic－process－in－situ－using－immunocytochemical2
B) TUNEL in situ procedureB.2.1 Materialsproteinase K (pK) (A2), H2O2 , TdT buffer (A1), TdT enzyme (A2), biotinylated dUTP (A2), TB buffer (A1), seru
How－to－perform－automated－counts－of－fluorescently－stained－cells.
NoteThis protocol describes semi-automated cell counts using fluorescently labeled cells, a hemocytometer and ImageJ software. The hemocytometer is n
FACS－Analysis－Using－Peripheral－Blood－Cells
FACS Analysis Using Peripheral Blood CellsCollect blood (75 microliters) into 1ml PBS containing 5 microM EDTA (10 microliters of 0.5 M stock) and mix
Detection－of－Viruses－in－Infected－Plant－Extracts－using－ImmunocapturePCR
1) Immunocapture stageCoating buffer: 15 mM Na2CO3; 35 mM NaHCO3, and 3 mM NaN3, per liter (pH 9.6).Extraction buffer: (20 mM Tris-HCL (pH 8.0), 138
Detection－of－Viruses－in－Infected－Plant－Extracts－using－ImmunocapturePCR
1) Immunocapture stageCoating buffer: 15 mM Na2CO3; 35 mM NaHCO3, and 3 mM NaN3, per liter (pH 9.6).Extraction buffer: (20 mM Tris-HCL (pH 8.0), 138
PBMC细胞的精确计数和活性分析（三）
五、使用仪器发表文章AuthorDateTitleJournalCell TypeCellometer / ApplicationsMahato, Ram INovember 2013Synthesis and Characterization of an Anti-Apoptotic Immu
免疫细胞化学
Introduction to Immunocytochemistry (House Ear Institute)A brief overview of common available methods. BrDU Immunocytochemistry using peroxidase and
ELISPOT－(Enzymelinked－ImmunoSPOT)
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, bu
实验室自动化与筛选协会2013亚洲会展展商技术论坛
第一天(2013年6月6日)13:45-14:30 展商技术论坛 1： 会场：宴宾厅 II，2楼 议题：Cell-based assays and 3D cell culture：Your body is in 3D – Why is your cell cult
Dual－and－TripleCo...－（二）
主要试剂1. Blocking reagent after coating: 10% fetal calf serum (FCS) in phosphate-buffered saline (PBS), pH 7.4.2. Cells suspended in complete culture me
细胞遗传学——原位杂交(ISH)
In Situ Hybridization· In Situ Hybridization (jsmith1@po-box.mcgill.ca)In situ hybridization, as the name suggests, is a method of localizing,
ELISPOT－Protocol
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on t
ELISPOT－Protocol
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on t
Protocol－for－Dual－Pulse－Labeling－Using－EdU－and－BrdU－Incorporation
实验概要The measurement of cell proliferation is fundamental to the assessment of cell health, genotoxicity, and drug efficacy. Proliferation is traditi
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement
IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr [1]. It plays an important role in embryonic development, maintenance o
DualColor－ELISPOT－Assay－for－the－Simultaneous－Detection1
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T CellsINTRODUCTIONThe enzyme-linked immunospot (ELISPOT) assay
信号传导
Cytokine Bioassays (eBioscience)Biological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary c
信号传导
Cytokine Bioassays (eBioscience)Biological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary c
Simultaneous－analysis－of－DNA－content
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques. William Telford. Louis E. King and Pamela