Nucleofection

This is an extract of the Amaxa Biosystems protocol Vs. 09-2005 optimized for use with the UC06 cell line. It is suggested that you try all 5 programs (A-23, A-27, A-13, A-12, B-16) with each Nucleofection solution initially to determine the optimal program and solution for your specific hES strain.Preparation of samplesCollect hESC as for normal culture protocol using collagenase. Wash the cells with PBS after harve......阅读全文

Nucleofection

This is an extract of the Amaxa Biosystems protocol Vs. 09-2005 optimized for use with the UC06 cell line. It is suggested that you try all 5 programs

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