Theribonucleaseprotectionassay(RPA)
The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by the discovery and characterization of DNA-dependant RNA polymerases from the bacteriophages SP6, T7 and T3, and the elucidation of their cognate promoter sequences. These polymerases are ideal for the synthesis of high-specific-activity RNA probes from DNA templates becau......阅读全文
MTT－Cell－Proliferation－Assay
MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, first described by Mosmann in 1983, is based on the ability of a mitochondri
Enzyme－Kinetics－assay－of－the－WT
To assay 17 b-HSD activity in lysates, cells were harvested 48h after transfection using PBS Enzyme Free Cell Dissociation Solution ( specialty Media
Cell－Clonogenic－Survival－Assay
DescriptionAllows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation. Procedure1. Grow
Nucleotide－Binding/Hydrolysis－Assay
MaterialsNucleotide mixMotor (50 - 100 µM; purity > 95%)0.5 M Tris-OAc, pH 7.510 mM EGTA10 mM MgCl2DDWSephadex G-50 Medium column (0.8 cm in x 20 cm)C
Gel－Shift－Assay－Systems
ProtocolsDownloadprotocol183kbpdf?Abstract for Gel Shift Assay SystemsThe gel shift, or electrophoretic mobility shift, assay provides a simple and ra
Alanine－Transaminase－Activity－Assay
实验概要Alanine Transaminase (ALT) is a transaminase (EC 2.6.1.2) also called serum glutamic pyruvic transaminase (SGPT). Alanine Transaminase is found
Polyphenoloxidase－(catechol－oxidases)－assay
Browning of the cut surface of some fruits and vegetables is due the presence of a group of enzymes called polyphenoloxidases. These enzymes are relea
Biorad－Protein－Assay:－Bradford
Biorad Protein Assay: BradfordStandards: 1 mg/ml BSA stock- dilute 1:10 to get 0.1 mg/ml BSAAdd To get H-2O20 µl 2 µg/ml 780 µl40 µl 4 µg/ml 760 µl60
Angiotensin－Protein－Kinase－Assay
James Hardwick's angiotensin assay protocolThis specific procedure was developed to assay the activity of the Lck kinase expressed from a retrovir
Radioactive－DNA－Fragmentation－Assay
DESCRIPTION of the method:The DNA Fragmentation Assay allows to determine the amount of DNA that is degraded upon treatment of cells with certain agen
In－Vitro－Protein－Ubiquitination－Assay
Ubiquitination is one of the most important posttranslational modifications in all eukaryote organisms. Ubiquitin-activating enzyme (E1), ubiquiti
Protocol－for－Aortic－Ring－Assay
ProceduresCover a 48-well plate with Matrigel (100μl/well) of and incubate for 30 min at 37°C, 5% CO2.Sacrifice the1-2 month old mice/rats (WT/mutant
Invitro－Phagocytosis－Assay－of－Macrophages
IntroductionThe term phagocytosis itself describes its mean phage = engulfment; cytosis: cell process. In other words, phagocytosis is the cellular pr
Apoptosis－TUNEL－assay－(Paraffin－Sections)
Protocol for Paraffin Sections:Dewax paraffin sections:Incubate slides, 55°C, 30 min.Xylenes, 2 times, 2 min. each100% EtOH, 2 times, 2 min. each95% E
Chemotaxis－Assay趋化性实验
Springer Lab，The CBR Institute for Biomedical Research, Inc. Department of Pathology Harvard Medical Schoolhttp://cbr.med.harvard.edu/investigators/sp
XC－Assay－of－MoMLV－Virus－Stocks
MaterialsWildtype MoMLV virus aliquot. Stored at -80ºC.Medium: DMEM + 10% FBSNIH 3T3 TK- cellsXC cellsPolybrene 1000x stock = 4 mg/mL, sterile filte
The－UnderAgarose－Migration－Assay
overviewThe Under-Agarose assay is a useful method for observing the response of a cell population to one or more chemoattractant sources. The behavio
Apoptosis－TUNEL－Assay－(frozen－sections)
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Beg
NKcell－cytotoxicity－assay
Outline:To measure NK cell killing, suitable target cells are labeled with 51Cr, washed and incubated together with the killer cells (and treatments).
Chick－Chorioallantoic－Membrane－(CAM)－Assay
CAM ASSAYShell-less embryo cultureFertilized white leghorn chicken eggs (SPAFAS Inc., Norwich, CT) were received at day 0 andincubated for 3 days at 3
alamarBlue®－Cell－Viability－Assay－Protocol
实验概要Cell health can be monitored by numerous methods. Plasma membrane integrity, DNA synthesis, DNA content, enzyme activity, presence of ATP, and c
DNA－laddering－assay－for－treated－cells
Characteristics of this procedure:I found the procedure described by Gong et al. to be a convenient and successful method to detect DNA laddering in c
SOFT－AGAR－ASSAY－FOR－COLONY－FORMATION
Note: All volumes are calculated to cater for four plates per point.Base Agar1. Melt 1% Agar (DNA grade) in microwave, cool to 40 in a waterbath. War
IFNy－Elispot－Assay...
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on t
Endothelial－wound－healing－(cell－migration)－assay
DescriptionThis is a simple assay that can be used in any cell culture lab setup to test the effect of different compounds on endothelial cell migrati
James－Hardwicks－angiotensin－assay－protocol
This specific procedure was developed to assay the activity of the Lck kinase expressed from a retroviral vector in rat fibroblasts. You can obviousl
Assay－of－Tyrosine－Kinases－Using－Synthetic－Peptides
实验概要 Small synthetic peptide substrates are especially well suited for applications such as assays of tyrosine kinases in permeabilized cel
MINICHROMOSOME－MICROTUBULE－BINDING－ASSAY2
HYBRIDIZATION.Prehybridize blot at 65oC for ~3h in Church buffer containing 0.5mg/ml denature salmon sperm DNA (usually 14ml Church buffer plus 0.7ml
荧光素酶检测（Luciferase－assay）
Introduction Luciferase can be used as a reporter gene to measure the activity of promoters, and/or the transfection efficiency. Aims You will be prov
Assay－of－superoxide－dismutase－activity2
Cuvette holders in the sample chamber of the spectrophotometer were thermo-controlled at 25°C. For the blank test, 100 ml of 50 mM potassium phosphate