InvitroPhagocytosisAssayofMacrophages
IntroductionThe term phagocytosis itself describes its mean phage = engulfment; cytosis: cell process. In other words, phagocytosis is the cellular process of engulfing solid particles by the cell membrane to form an internal phagosome, or "food vacuole". The phagosome is usually delivered to the lysosome, an organelle involved in the breakdown of cellular components, which fuses with the phagosome. The con......阅读全文
Invitro－Phagocytosis－Assay－of－Macrophages
IntroductionThe term phagocytosis itself describes its mean phage = engulfment; cytosis: cell process. In other words, phagocytosis is the cellular pr
Erythrophagocytosis
OUTLINEThe erythrophagocytosis assay is performed to compare the phagocytic rate with and without anti-eythrocyte antibody in either macrophages from
InVitro－Adipocytes－Differentiation
IntroductionObesity is a significant clinical problem that contributes to life-threatening diseases such as diabetes and atherosclerosis. With an incr
Transfection－of－siRNA－oligos－to－peritoneal－macrophages
Use DeliverX Transfection from PanomicsModified protocol belowStep 1: Make complexesNote: Do not scale up more than 3xThaw siRNA and DeliverX reagen
Cadmium－induces－DNA－synthesis－and－proliferation－in－macrophages
Exposure to divalent cadmium ions (Cd2+) is a known cancer risk factor, but the molecular mechanisms responsible for the inappropriate induction of ce
流式细胞术检测细胞凋亡
实验概要Provides a rapid and convenient assay for apoptosis. 实验原理Apoptosis is a carefully regulated process of cell death that occurs as a normal part o
Aspartate－Assay
实验概要The Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted
Phosphate－Assay
1. Make standards using sodium phosphate at the following uM concentrations: 0, 2, 5, 7, 10, 20, 40, 60, and 80. Use the screw top glass tubes.2. Dry
Protease－assay
实验概要 In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in
MTT－Assay
This procedure is for cells in 96 well plates, if larger plates are used then adjust volumes accordingly.1 Make a solution of 5mg/ml MTT dissolved in
DGK－Assay
Buffers:- 2X buffer10 ml 0.5 M imidazol, pH 6.60.21 g LiCl1.25 ml 1 M MgCl21.0 ml 0.1 M EGTA, pH 6.6--> Bring volume up to 50 ml with distilled water.
Chemotaxis－Assay
PurposeThe purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cel
TUNEL－assay
PROTOCOL:•Deparaffinize and rehydrate slides:3 x 3´ Xylene3 x 2´ 100% ethanol1 x 2´ 95%, 80%, 70% ethanol (each)1 x 5´ 1x PBS•Microwave antigen retrie
Protease－assay
In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to softe
Bradford－Assay
Bradford AssayThe bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie B
Polygalacturonase－assay
This enzyme is famous for being involved in the development of the GMO tomatoes (more information from the link at the foot of this page). The cells o
Pectinase－assay
Pectinases are actually a mixture of enzymes, which, along with others such as cellulase, are widely used in the fruit juice industry where they are w
Motility－Assay
DescriptionVarious phenotypic characteristics are requiredfor a cancer cell to successfully complete the metastaticcascade. Among these, acquisition o
Bradford－Assay
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue
与乳腺癌相关的FCGR2A基因编码功能描述
该基因编码许多免疫应答细胞表面发现的免疫球蛋白Fc受体基因家族的一个成员。该基因编码的蛋白质是一种在巨噬细胞和中性粒细胞等吞噬细胞上发现的细胞表面受体，参与吞噬和清除免疫复合物的过程。选择性剪接导致多个转录变体。This gene encodes one member of a family of
FCGR2A基因突变与药物因子介绍
该基因编码许多免疫应答细胞表面发现的免疫球蛋白Fc受体基因家族的一个成员。该基因编码的蛋白质是一种在巨噬细胞和中性粒细胞等吞噬细胞上发现的细胞表面受体，参与吞噬和清除免疫复合物的过程。选择性剪接导致多个转录变体。This gene encodes one member of a family of
FCGR2A基因编码功能及结构描述
该基因编码许多免疫应答细胞表面发现的免疫球蛋白Fc受体基因家族的一个成员。该基因编码的蛋白质是一种在巨噬细胞和中性粒细胞等吞噬细胞上发现的细胞表面受体，参与吞噬和清除免疫复合物的过程。选择性剪接导致多个转录变体。This gene encodes one member of a family of
与肺癌相关的FCGR2A基因编码功能描述
该基因编码许多免疫应答细胞表面发现的免疫球蛋白Fc受体基因家族的一个成员。该基因编码的蛋白质是一种在巨噬细胞和中性粒细胞等吞噬细胞上发现的细胞表面受体，参与吞噬和清除免疫复合物的过程。选择性剪接导致多个转录变体。This gene encodes one member of a family of
Actin－Capture－Assay
David AmbergDialyze purified GST fusion proteins and actin into PBS + 1mM MgCl2 .Mix 5ug actin into 50ul total volume binding buffer.Mix 5ug GST-fusio
Assay－for－the－Micrococcal－Nuclease
Method: Essentially that described by Heins et al. (1966) based upon the release of acid soluble oligonucleotides following nuclease digestion of DNA.
Glucosamine－Rapid－Assay
Glucosamine Rapid AssayMRTHOD:Place sample (containing 0.5 - 10 µg GlcN) in a Pyrex screw capped tube.Add HCl to a final concentration of 2N and a fin
LOWRY－PROTEIN－ASSAY
The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al., J. Biol. Chem. 193: 265-
Bradford－protein－assay
Bradford protein assayConsiderations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly
Tube－formation－assay
DescriptionThis is a fast and easy assay to test the angiogenic/anti-angiogenic properties of molecules. As compared to other angiogenesis assays, suc
Noble－Agar－Assay
DescriptionCancer cells do not show anchorage and contact inhibition of growth. To assess the anchorage and contact independent growth of cells, noble