RadioactiveDNAFragmentationAssay
DESCRIPTION of the method:The DNA Fragmentation Assay allows to determine the amount of DNA that is degraded upon treatment of cells with certain agents, e.g. with TNF-alpha or anti-Fas antibody (IPO-4).The principle of this assay is:the cells' DNA is radioactively labeled by growing the cells in presence of 3H-Thymidine so that radioactive 3H-Thymidine is incorporated into the DNA. Then the labeled cells are inc......阅读全文
Radioactive－DNA－Fragmentation－Assay
DESCRIPTION of the method:The DNA Fragmentation Assay allows to determine the amount of DNA that is degraded upon treatment of cells with certain agen
DNA－Fragmentation－Assays－for－Apoptosis
Protocol I: Triton X-100 Lysis BufferIn 96 flat-wells plate, incubate 4x10 6 target cells (40 wells of 105 per well) with desired concentration of eff
Apoptotic－DNA－fragmentation－and－tissue－homeostasis
Apoptotic cell death can be triggered by many different cellular stimuli, resulting in activation of apoptotic signaling pathways including caspases (
QUALITATIVE－ANALYSIS－OF－DNA－FRAGMENTATION－BY－AGAROSE－GEL－ELECTROPHORESIS
1. IntroductionNuclear morphology changes characteristic of apoptosis appear within the cell together with a distinctive biochemical event: the endonu
DNA－methyltransferase－Assay
Methylated CpG island Amplification Protocol written by Minoru Toyota2. Materials2.1. MCARestriction enzymes SmaI, XmaIT4 DNA ligaseTaq DNA polymerase
Cellfree－System－for－the－examination－of－apoptotic－activity
IntroductionIn our lab we use the term 'cell-free system' when we talk about the examination of apoptotic activity in cytoplasmic extracts. T
QUALITATIVE－ANALYSIS－OF－DNA－FRAGMENTATION－BY－AGAROSE－GEL－ELECTROPHORESIS2
3. Commentary 3.1. Background informationApoptosis is an innate mechanism of eukariotic cell suicide which plays a major role in many physiological
DNA－laddering－assay－for－treated－cells
Characteristics of this procedure:I found the procedure described by Gong et al. to be a convenient and successful method to detect DNA laddering in c
Bench－Top－Radioactive－Work－Protocol
Log in radioactive material received and deduct amount of radioactive material used in each experiment in Radioisotope Log Book.Clear your bench top w
Methods－for－the－Measurement－of－a－Bacterial－Enzyme－Activity－in－Cell－Lysates2
Measurement of ACTase activityNuclear magnetic resonance spect roscopy (NMR)The unique potential of NMR spectroscopy for monitoring simultaneously the
体外转录
· In Vitro RNA Transcription (Promega)For in vitro preparation single-stranded RNA probes or microgram quantities of defined RNA transcripts f
Apoptosis:－A－Laboratory－Manual－of－Experimental－Methods－Andrea－Cossarizza
THE CELL 1. Morphological aspects of apoptosis Walter Malorni, Stefano Fais & Carla Fiorentini 2. Cell cycle Miriam Capri & Daniela BarbieriTHE NUCLEU
Guide－to－Cell－Proliferation－and－Apoptosis－Methods
Chapter 1: Cell Death - Apoptosis and Necrosis1.1Introduction21.1.1Terminology of cell death21.1.2Differences between necrosis and apoptosis31.1.3Apop
Screening－BAC－filters－with－nonradioactive－probes
(Protocol for a single high-density BAC colony filter (HDR filter) of 22 cmx22 cm) to be screened using Amersham''s ECL hybridization kit; thi
Aimees－nonRadioactive－EMSA－Protocol
NUCLEAR EXTRACTION OF TISSUE CELLSThis procedure was developed for HNEK cells grown in KGM, from Clonetics. All steps are performed on ice, with ice-
Methods－for－the－Measurement－of－a－Bacterial－Enzyme－Activity－in－Cell－Lysates3
Different enzyme assays for ACTase study in H. pyloriACTase properties were studied in situ in cell-free extracts to obtain information on enzyme func
Motility－Assay
DescriptionVarious phenotypic characteristics are requiredfor a cancer cell to successfully complete the metastaticcascade. Among these, acquisition o
DGK－Assay
Buffers:- 2X buffer10 ml 0.5 M imidazol, pH 6.60.21 g LiCl1.25 ml 1 M MgCl21.0 ml 0.1 M EGTA, pH 6.6--> Bring volume up to 50 ml with distilled water.
Protease－assay
实验概要 In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in
Chemotaxis－Assay
PurposeThe purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cel
Pectinase－assay
Pectinases are actually a mixture of enzymes, which, along with others such as cellulase, are widely used in the fruit juice industry where they are w
Protease－assay
In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to softe
Bradford－Assay
Bradford AssayThe bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie B
MTT－Assay
This procedure is for cells in 96 well plates, if larger plates are used then adjust volumes accordingly.1 Make a solution of 5mg/ml MTT dissolved in
Phosphate－Assay
1. Make standards using sodium phosphate at the following uM concentrations: 0, 2, 5, 7, 10, 20, 40, 60, and 80. Use the screw top glass tubes.2. Dry
TUNEL－assay
PROTOCOL:•Deparaffinize and rehydrate slides:3 x 3´ Xylene3 x 2´ 100% ethanol1 x 2´ 95%, 80%, 70% ethanol (each)1 x 5´ 1x PBS•Microwave antigen retrie
Bradford－Assay
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue
Aspartate－Assay
实验概要The Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted
Polygalacturonase－assay
This enzyme is famous for being involved in the development of the GMO tomatoes (more information from the link at the foot of this page). The cells o
凋亡细胞核DNA片段检测方法进展（二）
2.3 凋亡细胞的TUNEL和ISNT鉴定的流式细胞仪分析[16]对于培养的细胞，可以将TUNEL或ISNT鉴定同流式细胞仪结合起来分析其发生凋亡的情况。待检细胞与含有TdT或DNA聚合酶I或Klenow片段及生物素标记的dUTP反应液共孵育一段时间后，加入荧光素（常用FITC）标记的链霉抗生物