ProtocolforcompetitiveRTPCR
For quantifying mRNA, we use a competitive RT-PCR protocol with internal standard RNAs. These are added in a defined quantity to the RNA sample prior to the RT reaction. The resulting standard cDNA is coamplified with the same primers as the endogenous target sequence. Its PCR product is approximately 50 nucleotides smaller. This method allows measurement of small differences (as low as factor 2) in mRNA amount betwe......阅读全文
Protocol for competitive RT-PCR
For quantifying mRNA, we use a competitive RT-PCR protocol with internal standard RNAs. These are added in a defined quantity to the RNA sample prior
RT-PCR PROTOCOL
RT-PCR PROTOCOL材料与方法………………………………………………………… 1．材料 ………………………………………………………1.1 供试用组织(细胞)…………………………………1.2 主要仪器设备………………………………………1.3 主要试剂……………………………………………1.
Competitive RT-PCR Strategy for Quantitative Evaluation -1
Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I Quantiza
Competitive RT-PCR Strategy for Quantitative Evaluation -2
Determination of Accuracy of the Competitive PCR To test the precision of the results obtained with this competitive PCR, five different amounts of
Competitive RT-PCR Strategy for Quantitative Evaluation -3
Competitive RT-PCR in Different Tilapia TissuesAbundance levels of tiGHR I mRNA (target) in different tilapia tissues were measured using the quantita
Competitive RT-PCR Strategy for Quantitative Evaluation -4
We have also been able to detect expression of this receptor in all studied tissues, which is consistent with the pleiotropic nature of growth hormone
Competitive RT-PCR Strategy for Quantitative Evaluation -5
3. Characterization of the method precision and repeatability.a. Ensemble PCRs in the same conditions established before using quantities of target in
反向PCR
主要内容如下：· RT-PCR· Competitive and Quantative RT-PCR· In Situ RT-PCR· RL-PCR· DNA Contamination· RT-PCR
Competitive ELISA
DAY 11. Coat Nunc immuno-module plates overnight, in usual manner.2. Set up competition assay between antibody and competing substance :-Prepare a 1:2
METTLER TOLEDO Wins Frost & Sullivan Award For Competitive Strategy
METTLER TOLEDO Wins Frost & Sullivan AwardFor Competitive Strategy Innovation and LeadershipFrost & Sullivan has presented their 2014 Global Award f