WIKI资讯
WIKI资讯
Packaging of DNA into chromatin allows the cell to store its genetic information efficiently and has an important role in regulating gene expression. Recent studies have revealed that chromatin structure can be altered by covalent modification of nucleosomes, in particular acetylation and methylation of the core histone tails. Histone hyperacetylation usually characterizes active chromatin, while histone deacetylatio......阅读全文
The siRNA user guide (revised May 6, 2004) Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysa
Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysates (Tuschl et al. 1999), we have systematically analyzed t
随着人类基因组计划（human genome project ）在2003年顺利完成，基因组测序技术取得了长足的进步，这直接导致了每兆基因组成本的大幅下降以及检测的基因组数量越来越多。人们对基因组的复杂性深感震惊，这也引导着测序技术的进一步发展。最近的一些突破性技术使得测序技术在更短的时间内可以
应用WGS正在成为NGS中最广泛的应用。通过该技术并且结合生物学应用，研究人员可以获得基因组信息中最值得注意的信息73。举例来说，2012年，Ellis等报道了基因与乳腺癌患者芳香酶抑制剂（aromatase inhibitor）治疗法之间的关联。他们指出突变，后果与诊断之间的关联，同样还有
Whole-Genome and Custom Fine-Tiling Array CGHComparative Genomic Hybridization (CGH) measures DNA copy number differences between a reference genome a
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
2019年4月8日，中国科学院上海植物逆境生物学研究中心何跃辉课题组和杜嘉木组合作在Nature Plants发表题为“Embryonic resetting of the parental vernalizedstate by two B3 domain transcription facto
Argonaute - A family of proteins containing multiple domains and involved in RNA interference (RNAi). Argonatue is the main component of RNAi eff
截至2019年12月23日，中国学者在Cell，Nature及Science在线发表了107篇文章（2019年的Cell ，Nature 及Science 已经全部更新），iNature团队对于这些文章做了系统的总结： 按杂志来划分：Cell 发表了31篇，Nature 发表了44篇，Scie
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a vari
RNA interference (RNAi) is a phenomenon in which the introduction of double-stranded RNA (dsRNA) into a diverse range of organisms and cell types caus
美国当地时间2月8日，美国工程院公布2017年入选院士名单，共新增84位院士及22位外籍院士。其中有多位著名华人学者，包括黄永刚、沈向洋、张东晓、丛京生、陈向力等人。 美国国家工程院院士是工程界最高荣誉之一，授予那些在工程领域内从事研究、实践和教育并作出卓越贡献的人士；以及在新兴和发展中的技
TROUBLESHOOTINGNo products are visible in any lane.1. Potential Problem: PCR amplification is not initiated.Recommendations:a. Confirm that all PCR co
2019年上半年很快就结束了，iNature盘点了中国学者在Cell，Nature及Science发表的成果，我们发现总共有86篇（截至2019年6月24日），具体介绍如下： 4-6月发表的文章 【1】2019年6月21日，西北工业大学王文，中科院昆明动物研究所/BGI 张国捷及丹麦哥本哈根
实验概要This method is use to extract short RNAs from plant tissue. Some of the variables (e.g. centrifugation speeds×, precipitation
Acknowledgements The organizer of the workshop acknowledges Dr. Murray Milford, Professor and Interim Head, and Dr. Mark Hussey, Professor and In
利用Mastercycler X50系列PCR独有的2D梯度实现高效的PCR条件优化Ultimate PCR Optimization with Eppendorf Mastercycler® X50 2D-gradientArora Phang, Tim Schommartz,Eppe
RNAi GlossaryDicer - Dicer is a member of the RNase III family of nucleases that specifically cleave double-stranded RNAs. Dicer processes long d
Figure 2. Silencer ™ siRNA Validation Data Generated Using Applied Biosystems TaqMan® Gene Expression Assays. The indicated Silencer Validated siRNAs
Tumorigenesis is known to be a multistep process in which defects in various cancer genes accumulate. Epigenetic modifications, most importantly DNA m
在免疫学实验中，比如WB，IHC，IF等，大家都知道选着好正确的一抗，往往实验就成功了一大半，因此，往往很多研究者便会忽视对二抗，以及其它试剂盒品质的追求。二抗是一种相对而言比较稳定的产品，包装大，因此很多实验室都会公用一个二抗。但如果二抗没有选择好的话，影响的可能就是一整个实验室或者课题组了。艾美
The vitamin D receptor, VDR is the mediator of all genomic actions of vitamin D3 and its analogs. It belongs to a family of ligand induced transcripti
Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type IQuantizatio
The most recognized mechanism by which peroxisome proliferators regulated gene expresssion is through a PPAR/RXR heterodimeric complex binding to a pe
Hypoxia (or low O2 levels) affects various pathologies. First, tissue ischemia, a variation in O2 tension caused by hypoxia/reoxygenation, can lead to
The influence of methylation on the promoter activity and gene expression and the involvement of DNA methylation in carcinogenesis caused an extensive
Purpose:Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to h
经过特殊的算法，我们得到了2018年前10个月中国生物医学风云榜人物及最火爆的3个重大学术界事件，能够上榜的风云人物/事件，都曾长时间占据过100多个公生物医学公众号的头版头条。 在此，我们精选了其中的3个事件及16位风云榜人物。我们对其进行了划分，分别是：6星级的3个事件，分别位诺贝尔奖，国
Drug addiction is associated with long-term behavioral changes, suggesting a long-lived transcriptional regulator that responds to chronic drug exposu
Liver is the major site for carbohydrate metabolism (glycolysis and glycogen synthesis) and triglyceride synthesis (lipogenesis). While insulin was lo