Coimmunoprecipitationassays
co-IP assays can be performed between endogenous proteins or transiently or stably expressed exogenous - usually tagged - proteins. The advantage to using endogenous proteins is the avoidance of protein overexpression and tagging that can lead to artifacts. The advantage to using exogenously expressed tagged proteins is the generally high specificity of the anti-tag antibody and the ease with which a negative control......阅读全文
蛋白质磷酸化
Tyrosine Kinase Assay Using Synthetic Peptides (T. Miller)Small synthetic peptide substrates are especially well suited for applications such as assay
Preparation－of－Stroma,－Thylakoid－Membrane,－and－Lumen－Fractions－from－...
Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic AnalysisFor many studies regarding
Multiple－studies－with－a－single－experiment:－The－Power－of－...（六）
•SCX and high pH reversed phase fractionation are both orthogonal to low pH C18 LC separation•Strong cation exchange (SCX) requires sample desalting
Dynamic－Flow－Assay－in－a－Parallel－Plate－Flow－Chamber
Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850Flow assays allow visualization of c
The－Determination－of－Proteinprotein－Interactions－by－the－Matingbased－...
Dynamic and reversible protein–protein interactions have a pivotal function in all living cells. For instance, protein–protein interactions are in
转录分析的几种方法
信号通路，只有一个目的：将细胞的外部信号转换成细胞的内部变化。不管是胰岛素，还是异亮氨酸，抗原还是肾上腺素，它们的终极目的总是如出一则：对转录活性的改变。 这些对于转录活性的影响来自于转录因子与基因的调控区域：如启动子、增强子、沉默子等结合达到的。经典的凝胶迁移滞后试验（the electrop
The－Impact－of－Harmo...－（二）
In addition, proficiency panel projects have demonstrated that even after an experiment was done and spots were counted, considerable variation can
细胞黏附和迁移
Cell Adherence Assay (LTI)General and nice Protocol for cell adherence assay. Proteins are coated on microtiter plates and cells are added; after the
Critical－Appraisal－of－the－MTT－Assay－in－the－Presence－of－Rottlerin－6
Our experience indicates that it may not be sufficient to change the medium containing Rottlerin and to wash the cells before adding MTT to avoid a po
Growing－Cells－in－Geltrex™－Reduced－Growth－Factor－Basement－Membrane－Matrix
实验概要Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or
小鼠脾脏T细胞和人PBMCs的体外T细胞活化实验（二）
材料1X 无菌 PBSAnti-human CD3:Clone OKT3 (Functional Grade， eBioscience Cat. No. 16-0037) or Clone HIT3a (Functional Grade， eBioscience Cat. No. 16-0039)R
Ruedi小组用QTRAP开发出全球首个定量蛋白质组数据库
苏黎世理工学院借助AB SCIEX的QTRAP技术研发成功全球首个蛋白质组学MRM Atlas 基于质谱的在线数据库加速蛋白质分析 福斯特市，加利福尼亚—2010年10月4日，生命科学分析技术的全球领导者AB SCIEX公司，今天宣布瑞士联邦理工学院(苏黎世理工学院，ETH)与西雅
The－Impact－of－Harmo...－（一）
实验概要During more than 25 years of application in immunological sciences, ELISPOT has been established as a routine, robust, versatile, and reliable a
Isolation－and－Culture－of－Human－Brain－Tumor－Stem－Cell
The isolation, culture, identification, and purification of stem cells from primary human brain tumors of different phenotypes have marked capacit
蛋白质定量
Quantitative Determination of Peptides by Sulfhydryl (-SH) Groups New (Contributed by David Van Horn, Dept. of Chemistry, UC Berkeley Greg Bulaj, Dept
夹心ELISA1
Enzyme-linked Immunosorbent Assays (ELISAs) combine the specificity of antibodies with the sensitivity of simple enzyme assays, by using antibodies or
OpenSPR如何革新流式动力学检测（一）
流式细胞仪是现代细胞生物学家和免疫学家不可缺少的优秀工具。它可以提供广泛的信息，帮助研究人员识别各种细胞的物理和化学特征。作为一种用途极为广泛的仪器，流式细胞仪有许多应用，包括细胞计数、分选、活力、细胞凋亡检测、膜电位甚至结合检测等。但是其无法直接检测分子间结合动力学，因此本文我们讨论了流式细胞仪的
Separation－of－Platelets－from－Whole－Blood
PurposeThis protocol describes how to isolate human platelets from whole blood. Isolated platelets are used for static adhesion assays, for flow chamb
Jacobs:Protocol－Total－Protein－Isolation－Using－RIPA－Lysis－Buffer
MaterialsRIPA buffer (RIPA buffer enables the extraction of cytoplasmic, membrane and nuclear proteins and is compatible with many applications, inclu
Assay－of－Tyrosine－Kinases－Using－Synthetic－Peptides
实验概要 Small synthetic peptide substrates are especially well suited for applications such as assays of tyrosine kinases in permeabilized cel
Gel－Shift－Assay－Systems
ProtocolsDownloadprotocol183kbpdf?Abstract for Gel Shift Assay SystemsThe gel shift, or electrophoretic mobility shift, assay provides a simple and ra
CCLM：LCMSMS分析尿液中的皮质醇
24小时尿游离皮质醇（UFC）测定可用于库欣综合征（CS）的筛查和随访。由于免疫分析方法缺乏对UFC测量的特异性，因此，目前推荐使用高效液相色谱联用质谱仪（LC-MSMS）。我们的研究目的是比较使用四个独立实验室中执行的四种LC-MSMS方法UFC测量的结果，以评估实验室间的一致性。将24小时尿样（
如何借助epMotion－5073l移液工作站完成微量体积的...（二）
Figure 1: epMotion 5073l worktable for qPCR setup Results and DiscussionThe automation of the qPCR setup using the 10 µL dispensing tool and the epM
碳水化合物分析
Carbohydrate Assay (Hancock Laboratory) (Accessible only by IE)This protocol is used to determine the relative amounts of LPS CHO present in a given s
红细胞裂解的实验方法步骤
IntroductionPrior to using lymphoid tissue cell suspensions for flow cytometric analysis and/or for in vitro functional assays, it is recommended to r
一种基于涡旋射流装置的免疫印迹分析方法
免疫印迹分析（immunoblot assays，IAs）广泛用于生物标志物的检测，然而传统方法成本较高、耗时长、操作相对复杂，并且容易受到背景干扰，影响检测灵敏度。 近日来自加利福尼亚大学的研究团队研发基于涡旋射流装置（vortex fluidic device，VFD）的免疫印迹分析方法，
关于钙离子和镁离子对胰蛋白酶活性抑制作用的研究文献
推荐几篇可能相关的研究文献，您可以通过学术数据库（如 Web of Science、Scopus、PubMed 等）获取全文："The inhibitory effects of calcium and magnesium ions on trypsin activity: Insights fro
实验室自动化与筛选协会2013亚洲会展展商技术论坛
第一天(2013年6月6日)13:45-14:30 展商技术论坛 1： 会场：宴宾厅 II，2楼 议题：Cell-based assays and 3D cell culture：Your body is in 3D – Why is your cell cult
COPAS流式分选系统简介及其在药物筛选方面的应用
微型模式动物、模式植物种子、大体积细胞及微球的分选在生命科学研究中有着非常广泛的应用，但是由于这些对象体积太大，普通流式细胞仪难以对其进行分选，而手工镜下分选耗时耗力、效率低下、准确性难以保证，因此一种自动化大体积微粒分选系统应运而生，它就是本文介绍的COPASTM多参数生物微粒分析与分选系统。
线粒体荧光探针大全：TMRM，Mitotracker，JC1（4）
Nonyl Acridine OrangeNonyl acridine orange (A1372) is well retained in the mitochondria of live HeLa cells for up to 10 days, making it a useful probe