ZOOM®IPGRunner™系统：简化的双向电泳（2Dgeleletrophoresis）
摘要双向电泳（Two-dimensional（2D） gel electrophoresis）是一项基于蛋白的两种不同特性：电荷和质量来分离蛋白的技术。首先基于蛋白固有电荷，通过等电聚焦（isoelectric focusing IEF）进行第一向蛋白分离，然后根据蛋白的质量，在第二向中通过SDS-PAGE电泳进行蛋白分离。使用这两种不同的分离技术的结果是增高了对蛋白的分辨率。传统的2D技术费用高昂、操作烦琐和耗费时间。ZOOM® IPGRunnerTM系统提供了进行第一向等电聚焦简单而快速的方法，无需使用矿物油。使用7cm ZOOM®固定pH梯度胶条（immobilized pH gradient IPG strips）的第一向电泳和使用NuPAGE® Novex 4-12% Bis-Tris ZOOM® Gels的第二向电泳总共可以在3小时内完成。前言双向电泳（Two-dimensional（2D） gel electrop......阅读全文
ZOOM®－IPGRunner™系统：简化的双向电泳（2D－gel－eletrophoresis）
摘要双向电泳（Two-dimensional（2D） gel electrophoresis）是一项基于蛋白的两种不同特性：电荷和质量来分离蛋白的技术。首先基于蛋白固有电荷，通过等电聚焦（isoelectric focusing IEF）进行第一向蛋白分离，然后根据蛋白的质量，在第二向中通过SDS-
InGel－Digestion－of－Proteins－Separated－byPolyacrylamide－Gel－Electrophoresis
1. Excision of protein bands (spots) from polyacrylamide gelsRinse the gloves you use with water to avoid traces of dust in your sample.Rinse the gel
RNA－gel－electrophoresis
实验概要RNA gel electrophoresis主要试剂DEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide sol
Gel－Electrophoresis－of－DNA
What is Electrophoresis?Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab we
Agarose－gel－electrophoresis
General ProcedureCast a gelPlace it in gel box in running bufferLoad samplesRun the gelImage the gelCasting Gels0.7% agarose gel with 1kbp ladder in U
RNA－gel－electrophoresis
MaterialsDEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide solutions should also be
Agarose－Gel－Electrophoresis
实验概要Separating nucleic acid fragments by agarose gel electrophoresis.实验原理 Agarose gel electrophoresis remains the most widely used technique for sep
Polyacrylamide－Gel－Electrophoresis－of－Oligonucleotides
1. Pour and polymerize a 20% polyacrylamide gel, no Urea.2. Remove clamps. Rinse with water. Remove comb. Rinse top of gel well.3. Insert comb teeth d
Blue－Native－Gel－Electrophoresis
Blue Native Gel ElectrophoresisStock solutions49.5%T, 3%C Acrylamide 24 g acrylamide, 0.75 g bisacrylamide / 50 ml H2O Store at RT3 x Gel buffer 150 m
Agarose－Gel－Electrophoresis－of－DNA
1) Dissolve 1 g of agarose in 100 ml of 1X TAE or TBE buffer (gives a 1% gel). See note for making LMP agarose gel. 2) Cast the gel with the comb in p
Alkaline－agarose－gel－electrophoresis
Alkaline agarose gel electrophoresis (Sambrook et al., 1989)Alkaline agarose gels can be used to determine the size and quality of first and second st
Gel－Shift－Assay－Systems
ProtocolsDownloadprotocol183kbpdf?Abstract for Gel Shift Assay SystemsThe gel shift, or electrophoretic mobility shift, assay provides a simple and ra
Protein－concentration－of－Laemmli－gel－samples
Protein concentration of Laemmli gel samplesTo 10 µl boiled lysate (in Laemmli sample buffer) add 40µl water + 50µl 50% TCA. Ppt. 10 min. on ice. Spin
High－Resolution－Agarose－Gel－Electrophoresis
实验概要Agarose gel electrophoresis remains the most widely used technique for separating nucleic acid fragments due to its ease of use, non-toxicity, a
Denaturing－Gradient－Gel－Electrophoresis－(DGGE)
Purpose:Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to h
SSR－GEL－and－Silver－Staining－Protocol
I. EQUIPMENT:DNA sequencing unit (35 x 45 cm) & 2000V power supplyClampsLg. plastic trays (4), about 43 x 50 x 8 cm, and one lidTwo rocking platformsH
Acrylamide－Urea－Gel－(35－ml)
Acrylamide Urea Gel (35 ml)10%15%40/2% acrylamide10 ml13.1 ml10X TBE3.5 ml3.5 mlUrea15 g15 gH2010ml7.0 ml Microwave ~10 seconds and stir until dissolv
Denaturing－Agarose－Gel－Electrophoresis－of－RNA
The overall quality of an RNA preparation may be assessed by electrophoresis on a denaturing agarose gel; this will also give some information about R
SDS－Gel－Electrophoresis－of－Tubulin\MAPs
MaterialsStock Acrylamide: (30%T:0.8%C)30% by weight of acrylamide0.8% by weight of N,N'-bis-methylene acrylamideSeparation Gel (Final Concentrati
RigExpert－AA55－ZOOM天线分析仪是怎么运作的？
这款RigExpert AA-55 ZOOM选项蓝牙是在乌克兰制造的，保修期2年。是您在现场的实验室，它能够与智能手机或平板电脑/上网本配合使用。 下面让我们来看看RigExpert AA-55 ZOOM天线分析仪是怎么运作的？ RigExpert AA-55 ZOOM选件蓝牙的
RigExpert－AA55－ZOOM天线分析仪是怎么运作的？
这款RigExpert AA-55 ZOOM选项蓝牙是在乌克兰制造的，保修期2年。是您在现场的实验室，它能够与智能手机或平板电脑/上网本配合使用。 下面让我们来看看RigExpert AA-55 ZOOM天线分析仪是怎么运作的？ RigExpert AA-55 ZOOM选件蓝牙的
2D－Polyacrylamide－Gel－Electrophoresis
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
EGel®－CloneWell－Agarose－Gels
实验概要Instructions are provided below for using the E-Gel®CloneWell pre-cast agarose gels with the E-Gel® iBase™ Power System. For detailed instructio
Native－gel－electrophoresis（非变性电泳）
Native gel electrophoresis Under native PAGE conditions, polypeptides retain their higher-order structure and often retain enzymatic activity and inte
Electrophoresis－of－PCR－products－with－Sunrise－gel－apparatus
Electrophoresis of PCR products with Life Technologies Sunrise gel apparatusGel: In a 500 ml Pyrex® glass bottle, add:Agarose:3 gH2O270 mls10X TA30 ml
琼脂扩散试验（gel－diffusion－test）
可溶性抗原与相应抗体在半固体琼脂凝胶内扩散，二者相遇，在比例合适处形成白色沉淀。基本类型有单向扩散和双向扩散两种。 （一）双向琼脂扩散试验（Double immunodiffusion test） 抗原和抗体加到琼脂板上相对应的孔中，两者各自向四周扩散，如两者相对应，浓度比例合适，则经一定时间后，在
胶体电泳法（gel－lectrophoresis）方法步骤
SDS 平板胶片铸造︰1） 整理出两组玻璃片及白板铸胶组合，先用酒精拭净，并选择合适的间隔条（0.75 mm） 组合起来。请熟悉铸胶三明治组合的正确组装方式，以免灌入的胶液漏出来。2） 三明治组合后直立站好，准备所要浓度的SDS 胶体溶液如表4.2. 两片0.75mm 厚的平板胶片约需10 mL 分
凝胶过滤(gel－filtration,GF)的应用
1.生物大分子的纯化凝胶过滤是依据分子量的不同来进行分离的，由于它的这一分离特性，以及它具有简单、方便、不改变样品生物学活性等优点，使得凝胶过滤成为分离纯化生物大分子的一种重要手段，尤其是对于一些大小不同，但理化性质相似的分子，用其它方法较难分开，而凝胶过滤无疑是一种合适的方法。例如对于不同聚合程度
QUALITATIVE－ANALYSIS－OF－DNA－FRAGMENTATION－BY－AGAROSE－GEL－ELECTROPHORESIS
1. IntroductionNuclear morphology changes characteristic of apoptosis appear within the cell together with a distinctive biochemical event: the endonu
Ingel－digestion－of－proteins－for－peptide－fingerprint－mapping
Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples. Moreover, the development of two-dimension