EasyWaytoCloneGenesFromaPhageLibrary

Easy Way to Clone The protocol is oriented towards a C. albicans genomic library I made in Lambda Zap II on 7/97.The overall sequence of events is: • Titer and plate out phage • Lift plaques onto filters and prepare them for screening • Make a probe • Hybridize the probe to the filters • Wash the filters and expose to film • Purify putative plaques • Excise plasmid from the des......阅读全文

其它PCR方法

·         Standard PCR Protocol (Molecular Biology Techniques Manual)The followings are described in detailRecommended Reagent ConcentrationsRecommend

Streaking-Bacteria-for-Single-Colonies

1. Initial inoculum:- From solid media, touch an isolated colonies with a sterile applicator or toothpick.- From liquid media, touch the culture with

Extraction-of-25NT-RNA

实验概要This  method is use to extract short RNAs from plant tissue. Some of the  variables (e.g. centrifugation speeds×, precipitation times and  vo

Fluidigm公司微液流芯片在单细胞研究中的应用(二)

It was also worth it for Tannishtha Reya, who studies stem cell fate and cancer at Duke University Medical Center in Durham, North Carolina. "Im

1cm比色皿可置CUVALLUV-4WAY比色皿支架

1cm比色皿可置CUV-ALL-UV 4-WAY比色皿支架1cm比色皿可置CUV-ALL-UV 4-WAY比色皿支架配有四个光圈f/2的石英准直透镜,每个准直透镜上配备有光纤连接器,通过其与光纤相连来读取或照亮样品。当与Ocean Optics的模光谱仪和光源配套使用时,CUV-ALL-UV

ALK-in-cardiac-myocytes

Heart formation is cued by a combination of positive and negative signals from surrounding tissues. Inhibitory signals that block heart formation in a

Pest-Control

We use Dimilin G (4% diflubenzuron) from Maag AG to fight the small dipteran black flies (Trauermücken). It is a granular substance and sprinkled

PCR实验指导与常见问题分析2

Fig. 11. Example of the influence of extension temperature. Multiplex PCR with mixtrues A-B using two different PCR programs. Reactions on the right s

组织学——组织制备

·         Histological techniques (William H. Heidcamp)Very detailed guide to histological techniques, like  fixation, dehydration, embedment and subs

Isolation-of-human-primary-ovarian-surface-epithelial-cells

1. Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institutional Review (References)

A-Guide-to-CORNET-for-the-Construction-of-Coexpression-and-ProteinProtein..

To enable easy access and interpretation of heterogenous and scattered data, we have developed a user-friendly tool for data mining and integratio

Exposing-gels-and-plates-containing-radioactive-samples-to-Xray-film

Although most people use the PhosphorImager for western blots, kinase assays and methionine-labeled samples, X-ray film remains the best way to expose

Assay-of-superoxide-dismutase-activity1

Assay of superoxide dismutase activity by combining electrophoresis and densitometryAbstract. A modified technique was developed to assay superoxide d

JC1分析线粒体膜电位的方法

Analysis of Mitochondrial Membrane Potentialwith the Sensitive Fluorescent Probe JC-1 Andrea Cossarizza and Stefano Salvioli Department of Biomedical

Rab-GTPases-Mark-Targets-In-The-Endocytotic-Machinery

The eukaryotic cell contains compartments with distinct functions bounded by lipid bilayer membranes. The movement of membrane vesicles between these

Nature文献解读mRNAm6A甲基化调控对学习记忆功能机制的...

Nature文献解读-mRNAm6A甲基化调控对学习记忆功能机制的影响学习和记忆是大脑的基本功能,长时记忆的形成被认为需要神经元活动诱导下的蛋白质合成。以往的研究表明,m6A参与调节神经功能,包括小鼠中脑多巴胺能信号传导、飞虫飞行行为、成年小鼠神经生成和轴突再生。在大脑发育、行为体验和记忆形成过程中

An-Introduction-to-Biocomputing

An Introduction to BiocomputingVersion 2.01October 1996David Steffen, Ph.D.President, Biomedical Computing, Inc.6626 WestchesterHouston, Texas 77005US

-Genes--Dev.:生物钟机制可助慢性肺病治疗

  人们对生物钟机制并不陌生,地球上几乎所有生物的生理节奏都会受到该机制的影响。最近,英国曼彻斯特大学研究人员在《基因与发育》期刊上发表论文称,他们研究发现,小鼠肺组织的抗氧化防御通路会因生物钟机制影响而有节律地开启或关闭,利用这一节律,或可有效提高慢性肺部疾病的治疗效果。   研究人员称,受生物

Genes-Dev解析致癌组蛋白突变引发癌症的分子机制

  来自清华大学医学院李海涛教授课题组7月31日在《基因与发育》(Genes&Development)杂志上发表了题为《Molecular basis for oncohistone H3 recognition by SETD2 methyltransferase》(甲基转移酶SETD2识别致癌组

Genes--Development:USTC复合物在piRNA转录中的功能

  这一研究在线虫中发现了一个参与piRNA转录过程的上游序列转录复合物(USTC复合物)。  piRNA是一类在动物中保守的非编码小RNA,在转座子沉默、生殖发育、基因表达调控和性别决定等过程中发挥重要作用。  来自中国科学技术大学生命科学学院光寿红课题组与剑桥大学的Eric Miska课题组合作

清华施一公Genes--Dev细胞凋亡研究新进展

  在2015年1月31日,由中国科学院院士和中国工程院院士评选的“2014年中国十大科技进展新闻”在京揭晓,清华大学生命科学学院施一公院士课题组完成的“首次揭示阿尔茨海默氏症致病蛋白三维结构”而入选。2月2日,Elsevier 在其中文网站上公布“2014年中国高被引学者榜单”。中科院院

所有的看家基因(housekeeping-genes)的列表+引物设计服务2

NM_002128                Homo sapiens high-mobility group box 1 (HMGB1), mRNA    1295NM_002002                Homo sapiens Fc fragment of IgE, low aff

Genes--Devel:科学家揭示如何阻断癌细胞的转录?

  阐明介导子如何与TFIID串扰促进启动子-增强子之间的交流沟通对于揭示增强子功能的机制非常重要;近日,一篇发表在国际杂志Genes & Development上题为“The Pol II preinitiation complex (PIC) influences Mediator bindin

所有的看家基因(housekeeping-genes)的列表+引物设计服务3

NM_033142                Homo sapiens chorionic gonadotropin, beta polypeptide 7 (CGB7), mRNA                773NM_006351                Homo sapiens

Hints-and-precautions-for-the-care,-feeding-and-breeding-of-Neurospora

Some of these notes describe what may be established routines in older laboratories, but are either unpublished or published in inaccessible places or

皮肤上画一个传感器--测血糖so-easy!

  如果你认为测血糖一定要血糖仪?那你就凹土了!有一种神奇墨水,内含特殊传感器,可以帮助糖尿病患者监测血糖,时刻了解各种体检指标。该墨水可一次书写、多次阅读,实乃家中测毒必备产品,士兵侦测炸药和神经毒剂的良伴。  墨水成分  这么神奇的墨水出自加州大学圣地亚哥分校(UCSD),成果发布在2月26日的

Packagene®-Lambda-DNA-Packaging-System

Packagene® Lambda DNA Packaging SystemThe Packagene® Lambda DNA Packaging System is derived from the unique one-strain host system (Rosenberg). The Sy

Heterogeneity-of-SingleCell-Gene-Expression-Across-Phenotypically(一)

Introduction  Multi-cellular  populations are fundamentally driven by the collective properties of  individual cells. However, our understanding of ge

Isolation-and-Culture-of-Multipotent-Stem-Cells-from-Human-Bone-Morrow

Bone marrow contains three types of stem cells:1. Hematopoietic stem cells give rise to the three classes of blood cells that are found in the circu

Vacuum/Spin-Protocol-for-Isolating-DNA-from-Blood-with-Nucleated-Red

实验概要DNA  isolation from fish or avian blood sample can be difficult because it  contains nucleated red blood cells. E.Z.N.A. NRBC Blood DNA Kit is  de