Hintsandprecautionsforthecare,feedingandbreedingofNeurospora

Some of these notes describe what may be established routines in older laboratories, but are either unpublished or published in inaccessible places or scattered through the old literature, and thus may not be known to those just beginning to use Neurospora. Others may be practices unique to our lab. The attempt here is to supplement compilations of methods such as Davis and de Serres (1970 Methods in Enzymology 17A:7......阅读全文

Hints-and-precautions-for-the-care,-feeding-and-breeding-of-Neurospora

Some of these notes describe what may be established routines in older laboratories, but are either unpublished or published in inaccessible places or

Hints-and-precautions-for-the-care,-feeding-and-breeding-of-Neurospora2

9. Spontaneous germination. Some genotypes result in spontaneous ascospore germination (for example, the unpigmented ascospores of per-1 Type I). A si

The-Fungal-Genetics-Stock-Center-Methods

Some basic Neurospora methods compiled at StanfordVogel's Minimal MediumD. D. Perkins - Hints for the care, feeding and breeding of NeurosporaStra

Tips-and-hints-for-the-storage-of-antibodies

Storage of lyophilized antibodiesAll our products are shipped lyophilized (freeze-dried). Unlabeled antibodies are stable without loss of quality at a

Small-scale-DNA-preps-for-Neurospora-crassa.

Molecular biology experiments often require preparation of small amounts of DNA from many samples. This abbreviated DNA isolation method yields an ave

A-quick-RNA-miniprep-for-Neurospora-mycelial-cultures

Most RNA isolation techniques currently in use have been developed for the processing of large quantities of material. These typically involve multipl

An-Ultrafast-method-of-DNA-extraction-from-Neurospora

We have found that the DNA extraction procedure of Metzenberg and Baitch (Neurospora Newsl. 28:20)/Stevens and Metzenberg (Neurospora Newsl. 29:27) wh

Fast-and-reliable-miniprep-RNA-extraction-from-Neurospora-crassa

We have developed a method for isolating high quality total RNA from N. crassa mycelia that reliably yields large quantities. It is possible to extrac

Blood-collection-and-administration-of-fluids-and-drugs-(mouse)

Administration of Fluids and Drugs (Overview):Gastro-Intestinal Tract:Oral or per os (po)- - through the mouthGavage - - into the stomach via a feedin

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Guidelines-for-theUse-of-Analgesics-and-Tranquilizers-in-Laboratory-Animal3

BarbituratesThe barbiturates are an acid ring molecule with various ring substitutes that imbue the drug with different properties. Barbiturates are a

夹心ELISA2

General Protocol for the Sandwich ELISA method:Before the assay, both antibody preparations should be purified and one must be labeled.For most applic

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Dorsal-Root-Ganglion-Neuron-Culture-from-Adult-Rats

DescriptionProcedure for culturing and maintaining Dorsal Root Ganglion (DRG) neurons from adult rats. ProcedurePreparing Culture Surface- Collagen co

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Assessment-of-Magnaporthe-grisea-mating-type-by-spore-PCR

Isolation of DNA from filamentous fungi for PCR analysis is usually time consuming and involves use of toxic chemicals such as phenol/chloroform. In S

Phenol/chloroform-extraction

General InformationPhenol/chloroform extraction is an easy way to remove proteins from your nucleic acid samples and can be carried out in a manner th

Dynabeads®-Antibody-Coupling-Kit

实验概要The  Dynabeads and buffers provided in this kit will enable you to  covalently immobilize antibodies (or other protein ligands such as  lections,

Tissue-Culture-Methods2

IV. MAINTENANCECultures should be examined daily, observing the morphology, the color of the medium and the density of the cells. A tissue culture log

Growing-Cells-in-Geltrex™-Reduced-Growth-Factor-Basement-Membrane-Matrix

实验概要Basement  membranes are continuous sheets of specialized extracellular matrix  that form an interface between endothelial, epithelial, muscle, or

单克隆抗体

·         Tips and hints for the storage of antibodies (Synaptic Systems)·         Purification of IgG Using Protein A- or Protein G-Agarose(KPL)·