Sectioningstainedembryos.
The protocols for plastic and wax sections as used by the Vize lab. These protocols work, but they have not been optimized. If anyone has better protocols please let us know.Samples.Stain samples strongly, remember that in a section only the very strongest stain will be visible, even quite strong background will not be visible. BCIP/NBT is the only stain we have successfully used, we have tried Red new fuschin but it......阅读全文
Isolation－and－culture－of－rat－coronary－microvascular－endothelial－cells
CMVE were isolated from Wistar rat hearts by digestion of primary cell isolation kit.1. Hearts mounted on a Langendorff apparatus were perfused at 3
Betagal－staining－of－eukaryotic－cells－in－vitro
(Modification of methods of Dr. Seong-Seng Tan and Promega's "Protocols and Applications Guide")Cells previously transfected with a lac Z construc
Placental－trophoblast－and－chorionic－cell－cultures
1. Placental trophoblast and chorionic trophoblast cells were prepared using a modification of the method. 2. Term human placentae and chorion tissue
Isolation－and－characterization－of－rat－glomerular－endothelial－cells
Glomerular endothelial cells (GECs) from the kidney are in close juxtaposition to other cell types, such as mesangial cells, and may respond to as
显微冷冻切片机的使用和大（小）鼠脑组织切片操作实验
冷冻切片机可以用于：（2）切制薄而均匀的组织片；（2）组织用坚硬的石蜡或其他物质支持，每切一次借切片厚度器自动向前（向刀的方向）推进所需距离，厚度器的梯度通常为1微米。实验方法原理切制石蜡包埋的组织时，由于与前一张切片的蜡边粘着，而制成多张切片的切片条。实验材料大小鼠大脑试剂、试剂盒手术器械 显微冷
DNA转化实验指导2
1B. Cloning 1. A caveat on dephosphorylation: the most common reason for failure to obtain colonies is a result of adding too much BAP or CIP to
peptide－fingerprint－mapping
Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples. Moreover, the development of two-dimension
Detection－of－apoptotic－process－in－situ－using－immunocytochemical2
B) TUNEL in situ procedureB.2.1 Materialsproteinase K (pK) (A2), H2O2 , TdT buffer (A1), TdT enzyme (A2), biotinylated dUTP (A2), TB buffer (A1), seru
Ingel－digestion－of－proteins－for－peptide－fingerprint－mapping
Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples. Moreover, the development of two-dimension
Histone－blotting－protocol
实验概要 Western blot detection of histone proteins. 实验步骤 The following protocol refers to the western blot detection of histone proteins derived from p
免疫细胞表面抗原分子CD家族对照表(CD1CD247)
Exalpha Biologicals produces and sells products for Life Science Research, including monoclonal and polyclonal antibodies, for use in immunohistochemi
Typical－Western－Tra...
实验概要Peprotech provides a typical western transfer and development protocol.实验原理Western Transfer, also known as Western Blotting, is a rapid immunobl
Histone－blotting－pr...
实验概要The method provides a procedure and tips for detecting histone proteins.The protocol refers to the western blot detection of histone proteins d
使用CCCadvanced™FN1无异源耗材培养人多能干细胞（三）
Figure 2: hiPSCs proliferation during long-term expansionA. Cumulative cell population doublings for hiPSCs cultured on the FN1 motifs surface as we
流式细胞术检测细胞凋亡
实验概要Provides a rapid and convenient assay for apoptosis. 实验原理Apoptosis is a carefully regulated process of cell death that occurs as a normal part o
mice－islet－isolation
1. Islets of Langerhans were isolated from 5- to 7-week-old nonobese diabetic (NOD) mice. 2. Which involves cannulation of the common bile duct and
Isoelectric－Focussing－of－Membrane－Proteins－by－Slab－Gel－Method
REFERENCE: Ames, G.F.L. and Nikaido, H. 1976. Biochemistry. 15:616-623.MATERIALS:Gel solution:1.05 gacrylamide0.032 gbis-acrylamide8.25 gurea6.5 mldis
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells2
Flow Cytometric AnalysisPrior to flow cytometric analysis, the vital dye Hoechst 33342 (Sigma-Aldrich, St. Louis, MO, USA) was added to cell suspensio
Slice－and－Explant－Culture－Protocols－–－Hevner－lab－2002
for axon tracing & cell culture studies in vitro using embyros age E11.5 – E16.5modified from Rubenstein lab and Price lab protocols1. Setupa. 2 hr be
Laser－Capture－Microdissection－(LCM)
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
Isolation－of－human－prostatic－smooth－muscle－cells
Human prostate tissues1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of the prostate. 2.All
使用CCCadvanced™FN1无异源耗材培养人多能干细胞（二）
Materials and MethodsCell culture conditions and surface transitionCryopreserved hiPSCs (SC102A-1, SBI™, USA) were initially thawed and pre-cultivat
Comparative－assessment－of－glycosylation－of－recombinant－human－...（二）
Experimental sectionChemicals and reagentsOne lot of PuregonR-HP of 50 IU/0.5mL and two lots of PuregonR-HP of 100 IU/0.5mL (rhFSH) (Organon, Oss, N
Chorioallantoic－membrane－grafting－with－chick－embryo－limb－buds
ObjectiveThis experiment explores the ability of the chick chorioallantoic membrane (CAM) to support an excised limb bud from a donor embryo. The chic
Growth,－Maintenance－and－Transfection－of－Suspension－Adapted－293EBNA－cells
ProcedureI. INTRODUCTIONThe 293 EBNA cell line is established from primary embryonal human kidney cells transformed with sheared human adenovirus type
“女性”更需注意哦，频繁喝酒会杀死大脑干细胞！
前两天，探索君发布了一篇《权威机构确认：酒精是明确的致癌因素》（详细），得到了大家的广泛关注。事实上，酒精滥用会对大脑产生有害影响，导致认知和行为功能上的缺陷，以及与酒精相关的神经退行性疾病。很多因素影响着大脑对酒精的响应，包括年龄、性别、饮酒时间、饮酒量等。 神经干细胞（Neural ste
3－Color－Staining:－AlphaCatenin－in－HeLa－Human－Cervical－Cancer－Cells
实验概要Alpha-catenin in HeLa human cervical cancer cells was labeled using mouse anti-α-catenin and visualized with Alexa Fluor® 488 goat anti-mouse Ig
西安光机所三维显微成像技术研究取得新进展
日前，Nature旗下的Scientific Reports 刊登了中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室姚保利研究组题为Full-color structured illumination optical sectioning microscopy 的研究论文。 众
Autoradiography
MaterialsH-Thymidine, specific activity of 2.0curie/millimoleOnion sets, jars and toothpicksAlcohol-acetic acid fixativeMaterials for feulgen reaction
Invitro－Phagocytosis－Assay－of－Macrophages
IntroductionThe term phagocytosis itself describes its mean phage = engulfment; cytosis: cell process. In other words, phagocytosis is the cellular pr