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USE OF THE LIGHT MICROSCOPEEach time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjust the iris diaphragm and focus the condenser to give its optimum performance, this could result in objects in the preparation being undetected.There are many microscope set up procedures for specific functionality but the most versatile for general use is ‘Critical Illu......阅读全文
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C. Restriction digestionRestriction enzyme digestions are performed by incubating double-stranded DNA molecules with an appropriate amount of restrict
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Anesthetic MachineThe best method of delivering an inhalant anesthetic is with an anesthetic machine. These machines precisely mix the gas with air o
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暗视野显微镜（dark field microscope）是光学显微镜的一种，也叫超显微镜（ultramicroscope ）。暗视野显微镜（dark field microscope）的聚光镜中央有挡光片，使照明光线不直接进入物镜，只允许被标本反射和衍射的光线进入物镜，因而视野的背景是黑的，物
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Remove Kodak NTB2 nuclear emulsion from fridge and place at 42oC for around 30-60 mins (until melted).Make up the developer and the fixer and place in
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Remove Kodak NTB2 nuclear emulsion from fridge and place at 42oC for around 30-60 mins (until melted).Make up the developer and the fixer and place in
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Full size image (70 KB)In vitro incubation of a reaction mix that contains labeled endosomes, cytosol and an ATP-regenerating system at physiological
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