WIKI资讯
WIKI资讯
Maintenance of Cell CultureAuthor: Nanci DonackiSource: Contributed by Nanci DonackiDate Added: Tue May 14 2002Date Modified: Tue Apr 27 2004Abstract: Detailed procedure for culture and subculture cell in flask and plates PurposeTo describe the maintenance of cells in culture.SafetyEquipmentLaminar flow hoodCO2 IncubatorMechanical PipettorInverted microscopeVacuum pump and flaskMaterials......阅读全文
常规操作(主要内容如下)· Aseptic Technique· Culture Ves
Seeding After cells are thawed:NOTE: Do not dispense the entire contents of the cryovial into one T-25 flask!!Remove the cap, being car
ProcedureI. INTRODUCTIONThe 293 EBNA cell line is established from primary embryonal human kidney cells transformed with sheared human adenovirus type
截至2019年12月23日，中国学者在Cell，Nature及Science在线发表了107篇文章（2019年的Cell ，Nature 及Science 已经全部更新），iNature团队对于这些文章做了系统的总结： 按杂志来划分：Cell 发表了31篇，Nature 发表了44篇，Scie
3. If your result falls into any quadrant other than the "High Yield-High Viability" quadrant, refer to Appendix D, Improving Cell Yield and
Pulmonary alveolar type II cells carry out highly specialized functions that include the synthesis, secretion, and reutilization of surfactant, a
IntroductionObesity is a significant clinical problem that contributes to life-threatening diseases such as diabetes and atherosclerosis. With an incr
IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr [1]. It plays an important role in embryonic development, maintenance o
Corneal keratocytes (corneal fibroblasts) that this corneal layer is specialized fibroblasts residing in the stroma, representing about 85-90% of
1. Bladders were excised from deeply anesthetized (urethane, 1.2 gm • kg−1, i.p.) Sprague Dawley rats (of either sex), cut open, and gently s
Flow cytometry analysis of the quantitative expression of 3 key pluripotency-associated transcription factors (Nanog, OCT3/4 and SOX2) complemen
“We picked 42 genes to validate on the BioMark system,” Dr. Yao said. “We picked them to represent different functional categories.”“We used the F
G. Bacterial cell maintenanceFour strains of E. coli are used in these studies: JM101 for M13 infection and isolation (4), XL1BMRF' (Str
E. Elution of DNA fragments from agaroseDNA fragments are eluted from low-melting temperature agarose gels using an unpublished procedure first develo
实验概要Important—Please read!GIBCO® Fresh Hepatocytes are shipped in cold preservation medium designed to keep cells viable at 4°C. This ship
实验概要Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, m
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zúñiga-Pfl
IV. MAINTENANCECultures should be examined daily, observing the morphology, the color of the medium and the density of the cells. A tissue culture log
ReagentsMedium A - Pre-fusion Medium and Hybridoma Expansion MediumMedium B - Fusion Medium Medium C - Hybridoma Recovery MediumMedium D - Hybridoma S
Author: Nanci DonackiSource: Contributed by Nanci DonackiAbstract: Procedure for establishing hybridoma in one stepReagents(StemCell Te
before start:thaw cells from liquid nitrogen, grow in 75cc flask (T75) in Fischer's medium MM (maintenance medium) until content gets cloudy and s
cell proliferation assaybefore start:thaw cells from liquid nitrogen, grow in 75cc flask (T75) in Fischer's medium MM (maintenance medium) until c
Preparing Overnight Bacteria Culture (LaboratoryExperiments.com)This is a basic procedure for high school students and useful for those who are n
ADSC isolation, culture, and cloningRat amnion membrane is mechanically separated from the chorion of embryonic day 18.5 Sprague–Dawley rat embryosThe
Purpose and BackgroundsCHO lec 3.2.8.1 cellsCHO Lec 3.2.8.1 cells have four independent mutations in the N- and O- glycosylation pathways (Stanley, 19