Observationoflivingandplasticembeddedchickembryos
The development of chick embryos has been studied since Aristotle. It is one of the most intensely studied organisms. One reason for this is that there are great similarities between avian and mammalian embryology. Another advantage is that the embryo is relatively accessible and can be operated on or treated with teratogens (substances that perturb development) while still in ovo(in the egg). Finally, fertilized chi......阅读全文
BTX特殊电极的应用汇总
BTX特殊电极的应用 此次只将贴壁转染、活体等进行总结，对于大规模的转基因的应用，由于BTX公司有新的发展，故此次未将其列入，待下次完善后补充。 1、 Adherent Cell Transfections (ACT)贴壁细胞转染 • In-situ electroporat
BTX特殊电极的应用汇总
BTX特殊电极的应用此次只将贴壁转染、活体等进行总结，对于大规模的转基因的应用，由于BTX公司有新的发展，故此次未将其列入，待下次完善后补充。1、Adherent Cell Transfections (ACT)贴壁细胞转染•In-situ electroporation of cells in t
Ultraviolet－irradiation－impairs－epiboly－via－microtubules－in－Zebrafish
IntroductionZebrafish have transparentembryos that develop outside the mother. They develop rapidly, so that at 24 hours after fertilization, the embr
Cell－death－detection－in－Xenopus－embryos－by－ELISA
Sample preparationWash embryos 1 x in 25% MMRRemove excess bufferAdd 10 volumes "incubation buffer", i.e. 50 µl for 5 embryosLyse the embryos by gentl
DEVELOPMENTAL－EFFECTS－OF－LITHIUM－CHLORIDE－ON－XENOPUS－EMBRYOS
PURPOSE: The objective of this experiment is to study the effect of lithium chloride treatment on early blastula xenopus embryos.INTRODUCTION: When th
Fate－Mapping－Axolotl－Embryos－in－Early－Gastrulation
BackgroundSince different amphibians execute gastrulation in different ways, the study of amphibian gastrulation has been complex. A popular method us
Protocol－to－Count－Cell－Number－of－Preimplantation－Embryos
Protocol to Count Cell Number of Preimplantation Embryos using Nuclear Staining with Hoechst 33342 or DAPI Introduction The following is a simple pro
Whole－mount－TUNEL－analysis－of－Xenopus－embryos
Fixation and pretreatmentDejelly albino embryos carefully in 2% Cystein (pH 7.8).Remove the vitellin membrane with two pairs of tweezers (or c
The－effect－of－lithium－chloride－on－Lytechinus－variegates－embryos
PurposeThis experiment will study effects of lithium chloride on sea urchin development, forcusing on archenteron formation.IntroductionGastrulation i
Preparation－of－fixed－embryos－for－immunocytochemistry－and－AP－staining
1. Transfer 50 ml of embryo cultures to centrifuge tubes. Spin at 1500 rpm for 5 minutes. Check that you can see a pellet of embryos at the bottom.Qui
Green－Fluorescent－Protein－as－an－Indicator－ofTransfection－in－Chicken－Embryos
Green fluorescent protein (GFP) is responsible for the bioluminescence of the Pacific Northwest jellyfish, Aequorea victoria. In A.victoria, the 27-kD
The－Effects－of－CalciumFree－Seawater－onthe－Development－ofSea－Urchin－Embryos
ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were pl
Regulative－Development－in－Axolotl－Embryos;－Splitting－the－Heart－Field
ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically, we will split the morphogenetic
Whole－mount－fluores...
实验概要The method provides a protocol for whole mount fluorescent immunohistochemistry. The advantage of using fluorescence to stain whole mount sectio
Investigating－the－effect－ofthe－teratogen－cyclopamine－on－Ambystoma－mexicanum
ObjectiveThis experiment attempted to determine the effects of the teratogen cyclopamine on the Shh signaling pathway in axolotl embryos. Groups of ax
Histochemical－staining－of－sea－urchin－embryos－for－(AP)－enzyme－activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity1. Obtain embryo samples, tube of AP substrate buffer and tu
Whole－mount－antibody－staining－ofzebrafish－embryos－formarkers－ofsegmentation
1. Dechorionate 26 hr embryos (pharyngula stage) carefully with two fine forceps.Transfer to fixative (1% formaldehyde in PBS). Fix for 1 hour rocking
Early－development－of－primary－motor－neurons－and－somites－in－Zebrafish－Embryos
Background:Zebrafish,or the teleost fish Danio rerio,is a rapidly developing organism that is apopular species for studying vertebrate development. Cl
Effect－of－UV－Treatment－on－Early－Development－of－Sea－Urchin－Embryos
Objective:To test the effects of UV radiation at 254nm on Sea Urchin embryo development after radiation at the 2-cell stage.Background:Sea Urchins exh
Segmentation－Clock
In most animal species, the anteroposterior body axis is generated by the formation of repeated structures called segments. In vertebrate segmentation
Tunel－Procedure－in－Bovine－Embryos－牛胚胎TUNEL检测凋亡
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go highe
AgrobacteriumMediated－Maize－Transformation:－Immature－Embryos－Versus－Callus
Transformation with Agrobacterium tumefaciens is the preferred method for delivery of transgenes into a wide range of plant species including maiz
转基因
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Core)Thi
Differential－Interference－Contrast
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)PrincipleDifferential interference microscopy requires several optical
Phase－Contrast－Microscopy
Phase Contrast MicroscopyPrincipleMost of the detail of living cells is undetectable in bright field microscopy because there is too little contrast b
Immunofluorescence－Whole－Mount
Objective:Immunohistochemistry allows visualization of antigens (usually proteins) within an embryo. Typically, a primary antibody binds specifically
FISH－protocols－for－Drosophila2
3. Methods3.1 RNA Probe Preparation1. Different strategies can be used to prepare template DNA for synthesizing antisense RNA probes by in vitro tr
The－effects－ofdifferent－concentrations－oflithium－chloride－on－thedevelopment
AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular Brachydanio rerio embryos results in
The－Effect－of－pH－Levels－By－Varying－the－Acidity－of－theExtracellular－Solution
BackgroundZebrafish, Brachydanio rerio, have a distinct embryonic development. The embryo goes through a process called epiboly. Epiboly, as defined i
Development－of－the－Heart－Morphogenetic－Field－in－the－Axolotl－Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d