RegulativeDevelopmentinAxolotlEmbryos;SplittingtheHeartField
ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically, we will split the morphogenetic field that is responsible for heart formation using grafted tissue from the gill area, and attempt to form two hearts – one on either side of the grafted tissue.BackgroundOrganisms that use regulative development rely on cell-cell interactions to determine wh......阅读全文
Regulative－Development－in－Axolotl－Embryos;－Splitting－the－Heart－Field
ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically, we will split the morphogenetic
Development－of－the－Heart－Morphogenetic－Field－in－the－Axolotl－Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
Axolotl－Protocol:－Transplantation－of－the－Gill－Organ－Field
Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ fie
Developmental－and－phenotypic－implications－of－transplantation
ObjectiveIn this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the
Axolotl－Microsurgery
I. Embryo Preparation1) Manually dejelly embryo of appropriate stage. Use fine foreps to break hole in hard jelly coat. Remove membrane around embryo
NFAT－and－Hypertrophy－of－the－heart－(Transcription－in－the－broken－heart)
Hypertrophy associated with both hypertension and obstruction to ventricular outflow leads to pathologic cardiac growth and it is associated with incr
Routine－Splitting－and－freezing－of－cells
1. Grow cells to subconfluence in a flask.2. Harvest as per normal and count.3. Spin down 5min 1.2K in benchtop. Resuspend at 1.0 X 106/ml in 10% DMSO
Dark－Field－Viewing
Dark Field ViewingPrincipleTo view a specimen in dark field, an opaque disc is placed underneath the condenser lens, so that only light that is scatte
Pulse－Field－Electrophoresis
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra
In－VitroCulture－of－Chicken－Heart
The chicken is a classic organism used to illustrate the principles of basic embryology. One of the developmental systems which has been examined in g
Actions－of－Nitric－Oxide－in－the－Heart
Nitric oxide (NO) has a number of important physiological actions in the cardiovascular system. In the heart, NO plays role in keeping the vessels pat
Fate－Mapping－Axolotl－Embryos－in－Early－Gastrulation
BackgroundSince different amphibians execute gastrulation in different ways, the study of amphibian gastrulation has been complex. A popular method us
Development－(Immuno...
实验概要Peprotech provides a development (immunostaining) protocol.实验步骤1. After the transfer is complete, incubate the membrane in blocking solution (3% N
Investigating－the－effect－ofthe－teratogen－cyclopamine－on－Ambystoma－mexicanum
ObjectiveThis experiment attempted to determine the effects of the teratogen cyclopamine on the Shh signaling pathway in axolotl embryos. Groups of ax
Hop－Pathway－in－Cardiac－Development
Homeodomain transcription factors comprise a large family of DNA binding factors that regulate transcription and development. Many homeodomain genes a
Solutions－for－Sea－Urchin－Development
Solutions for Sea UrchinASW: Artificial sea water (MBL)NaCl24.72 gKCl0.67 gCaCl2.2H2O1.36 gMgCl2.6H2O4.66 gMgSO4.7H2O6.29 gNaHCO30.18 gdH2Oto 1 literS
Melanocyte－Development－and－Pigmentation－Pathway
Studies with homozygous knockout mice have suggested that stem cell factor (SCF or KITLG), c-Kit, microphthalmia (Mitf), and B-cell CLL/lymphoma 2 (Bc
The－Effects－of－Ethanol－on－Zebrafish－Development
Introduction:The early stages of zebrafish, Danio rerio development are characterized by meroblastic dicoidal cleavage. Initially cell division only o
旷场实验－(open－field－test)
实验概要观察实验动物在新异环境中的自主行为、探究行为与紧张度。实验原理旷场（open field test）分析系统是观察研究实验动物神经精神变化、进入开阔环境后的各种行为，例如动物对新开阔环境的恐惧而主要在周边区域活动，在中央区域活动较少，但动物的探究特性又促使其产生在中央区域活动的动机，也可
旷场实验－(open－field－test)
实验概要 观察实验动物在新异环境中的自主行为、探究行为与紧张度。实验原理 旷场（open field test）分析系统是观察研究实验动物神经精神变化、进入开阔环境后的各种行为，例如动物对新开阔环境的恐惧而主要在周边区域活动，在中央区域活动较少，但动物的探究特性又促使其产生在中央区域活动的动机，也可
Effect－of－ethanol－on－development－of－Danio－reriro
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
The－effect－of－temperature－on－the－embryonic－development－of－sea－urchins
Objective: This experiment aims to discover if temperature has an effect on the rates of sea urchin embryonic development. By altering the external en
开场实验/敞箱实验（Open－field－test）
研究发现，动物在敞箱中，由于对于新异环境的恐惧，主要在敞箱内的周边部活动，而在中央区活动较少，但是动物的探究本性有必然促使它试图在敞箱的中央区活动（包括活动、饮水或进食），从而产生冲突行为。抗焦虑药物在不改变动物一般运动的情况下可以增加实验动物进入敞箱中中央区的次数和中央区停留时间，或者增加实验动物
Angiotensinconverting－enzyme－2－regulates－heart－function
Cardiovascular diseases are predicted to be the most common cause of death worldwide by 2020. The major regulator of blood pressure homeostasis is the
安捷伦授予Jennifer－Field教授“思想领袖奖”
2023年11月16日，北京——安捷伦科技公司（纽约证交所：A）近日宣布授予Jennifer Field博士“安捷伦思想领袖奖”，奖项旨在表彰其在有机微量污染物和PFAS领域的开创性工作。Jennifer Field博士现任俄勒冈州立大学农学院环境和分子毒理学系的教授。她专注于对天然和改造系统中
Developmental－Effects－of－Transplantation－of－Cell
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mos
Human－Embryonic－Stem－(ES)－Cell－Protocols—Splitting－Human－ES－cells－onMatrige
based on splitting onto on plateWarm collagenase media to 37°C in a water bath.Aspirate media off of cell culture plate.Add the following amount of co
Human－Embryonic－Stem－(ES)－Cell－Protocols——Splitting－Human－ES－cells－on－MEFs
based on splitting onto one plateWarm collagenase IV split media to 37 °C in a water bath.Aspirate media off of cell culture plate.Add the following a
The－Developmental－Effects－of－Nicotine－on－Chick－Embryos
More than 22 million American women smoke and approximately 25% of these women continue to smoke during pregnancy (Lambers and Clark 1996). Cigarette
ALK－in－cardiac－myocytes
Heart formation is cued by a combination of positive and negative signals from surrounding tissues. Inhibitory signals that block heart formation in a