PhaseContrastMicroscopy
Phase Contrast MicroscopyPrincipleMost of the detail of living cells is undetectable in bright field microscopy because there is too little contrast between structures with similar transparency and no color. Unless the specimen mount is extremely thin, dark field mode may distort details. However the various organelles show wide variation in refractive index, that is, the tendency of the materials to bend light, prov......阅读全文
Phase－Contrast－Microscopy
Phase Contrast MicroscopyPrincipleMost of the detail of living cells is undetectable in bright field microscopy because there is too little contrast b
Differential－Interference－Contrast
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)PrincipleDifferential interference microscopy requires several optical
Microscopes－in－Cell－Biology
Microscopes in Cell BiologyIntroductionMicroscopy has a major role in the study of cells. From the very beginning, researchers have tried to develop w
Isolation－and－characterization－of－rat－glomerular－endothelial－cells
Glomerular endothelial cells (GECs) from the kidney are in close juxtaposition to other cell types, such as mesangial cells, and may respond to as
ELECTRON－MICROSCOPY
E.M. PROCESSING SCHEDULE - EPOXY RESINFix tissue in 2.5% glutaraldehyde in 0.1M sodium cacodylate buffer at 4oC, for a minimum of 4 hours. Tissue shou
Light－Microscopy
The light microscope, so called because it employs visible light to detect small objects, is probably the most well-known and well-used research tool
Timing－of－Cycles
MaterialsMonolayer cultures grown in 75 mm culture flasks (Cells from Exercise 11.4 may be used, or cultures of tetrahymena, yeast, or algae may be us
相称显微镜简介
相称显微镜是一种增加对比度的方法。可以在不染色的情况下看到活体细胞。 相称显微镜，又叫相差显微镜（Phase contrast microscopy）。相差是一种增加对比度的方法，可以在不染色的情况下看到活体细胞。 相称显微镜是一种利用光的衍射和干涉现象将透过标本的光线光程差或相位差转换成肉
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
Microscopy－with－Oil－Immersion
Microscopy with Oil ImmersionPrincipleWhen light passes from a material of one refractive index to material of another, as from glass to air or from a
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
Immunofluorescence－/－Confocal－Microscopy－Protocol
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
Use－of－Transmission－Electron－Microscopy
Use of Transmission Electron MicroscopyOverviewA protocol describing the use of Zeiss EM9-S transmission electron microscopy is presented. MaterialZe
Generic－Fixation－for－Electron－Microscopy
Generic Fixation for Electron MicroscopyThe best way to fix a sample for electron microscopy is to follow a procedure developed and proven by others.
Immunofluorescence－/－Confocal－Microscopy－Protocol
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
Negative－Stain－Electron－Microscopy－of－Microtubules
Negative staining is a rapid, qualitative method for analyzing microtubule structure at the EM level. Because negative staining involves deposition of
Specimen－Preparation－for－Scanning－Electron－Microscopy
Specimen Preparation for Scanning Electron MicroscopyWe recommend consultation with one of the lab directors before preparing specimens. The methods p
Chlamydomonas－Fixation－for－Transmission－Electron－Microscopy
Chlamydomonas Fixation for Transmission Electron MicroscopySolutions:Chlamydomonas culture medium + 2% glutaraldehyde (5 ml medium + 0.9 ml 25% glutar
Silver:－TimeLapse－Microscopy
Pad Preparation1. Microwave 2% agarose (mix of low-melt and normal, to taste) in Thorn media (see below). (If you have used different percentages of a
Fixation－and－Embedding－of－Microtubules－for－Electron－Microscopy
(This procedure can also be used for virtually any material that must be pelleted prior to fixation and thin sectioning)Primary fix:2% glutaraldehyde
Tetrahymena－Fixation－for－Transmission－Electron－Microscopy
Tetrahymena Fixation for Transmission Electron MicroscopyPellet Tetrahymena cells in a clinical centrifuge.OPTIONAL: Suspend cells in HNMK (50 mM HEPE
Immunofluorescence－Microscopy－of－tissue－culture－cells
Immunofluorescence Microscopy of tissue culture cellsThese methods are written for direct staining of filamentous actin with bodipy FL-phallicidin and
Medpace协助白血病药物进入PhaseⅢ
Medpace是一家CRO公司， 成立于1992年， 总部位于美国，其致力于协助制药企业和生物技术公司将新药早日推向市场。最近，Medpace与Actinium制药公司签约，帮助推动癌症治疗药物开发的后期进展。 Medpace主要任务是协助完成150名患者针对lomab-B药物的临床三期试验，
Noble－Agar－Assay
DescriptionCancer cells do not show anchorage and contact inhibition of growth. To assess the anchorage and contact independent growth of cells, noble
蛋白纯化反相柱(reversed－phase－column)的选择
HP-RPC的选择首先要看蛋白质的分子量，20kDa左右的一般用C4或C8，再小一点的可以用C18，太大的蛋白并不适于反相分离。C18通用性最好，但是有时候保留过强可能会导致收率较低。如果目的蛋白不是很针对，可以考虑通用性最强的C18柱。一般来说，4.6mm的内径比较常见，250mm长的柱子比150
EPON－resin－mixture－for－transmission－electron－microscopy
EPON resin mixture for transmission electron microscopyFor Epon WPE 153:~120 ml~60 ml~30 mlMix A:Embed 81244 ml22.1 ml11.1 mlDDSA67 ml33.3 ml16.7 mlMi
免疫电镜(Immune－electron－microscopy)原理
（一） 原理 免疫电镜技术是免疫化学技术与电镜技术结合的产物，是在超微结构水平研究和观察抗原、抗体结合定位的一种方法学。它主要分为两大类：一类是免疫凝集电镜技术，即采用抗原抗体凝集反应后，再经负染色直接在电镜下观察；另一类则是免疫电镜定位技术。该项技术是利用带有特殊标记的抗体与相应抗原相结合，在电
Preparation－Of－Ciliated－Protozoa－For－Scanning－Electron－Microscopy
Preparation Of Ciliated Protozoa For Scanning Electron MicroscopyGeneral notes: The same procedures are used to fix and stain cells for SEM and for TE
免疫电镜(Immune－electron－microscopy)原理
（一） 原理免疫电镜技术是免疫化学技术与电镜技术结合的产物，是在超微结构水平研究和观察抗原、抗体结合定位的一种方法学。它主要分为两大类：一类是免疫凝集电镜技术，即采用抗原抗体凝集反应后，再经负染色直接在电镜下观察；另一类则是免疫电镜定位技术。该项技术是利用带有特殊标记的抗体与相应抗原相结合，在电子
反向微柱的准备Preparation－of－ReversedPhase－Microcolumns
INTRODUCTIONOne versatile strategy for sample cleanup prior to MALDI-MS analysis uses microscale columns designed for direct sample elution onto the M