SqueezingfemalesforEggs
Four hours after boosting with hCG, the females are ready to begin laying eggs. The best test is to see if there are eggs in the holding buckets. Also, the cloaca gets red and swollen like in X. laevis.We recommend precoating the dishes that you will be putting eggs into with a little bit of L15/CS (see testes isolation section for description of L15/CS). This facilitates the manipulation and transferring o......阅读全文
Squeezing－females－for－Eggs
Four hours after boosting with hCG, the females are ready to begin laying eggs. The best test is to see if there are eggs in the holding buckets. Also
Natural－Matings
For natural matings, single pairs of boosted males and females are placed together. We usually keep them on a darkened shelf away from disturbances, b
Obtaining－Embryos:－hCG－Injection
Ovulation is induced in X. tropicalis by injection of human chorionic gonadotropin (hCG). X. tropicalis requires a much smaller dose than does X. laev
In－Vitro－Fertilization
When we first started using X. tropicalis, in vitro fertilization had an extremely poor efficiency. However, with the careful selection of a mature ma
Isolation－of－Zebrafish－embryos
Zebrafish will mate and deposit fertilized eggs on the bottom of the tank at 'dawn'. They can be accustomed to lay at any convenient time by k
Basic－Methods－of－Culturing－Drosophila
实验概要Basic Methods of Culturing Drosophila实验步骤Stockkeeping1. Mechanics Most stocks can be successfully cultured by periodic mass transfer of a
从成体昆虫中分离活细菌的方法
Isolation of live bacteria from adult insectsHoracio M Frydman , hfrydman@princeton.edu, Associate ResearcherRelated Journal & Article InformationManu
从成体昆虫中分离活细菌的方法
Isolation of live bacteria from adult insectsHoracio M Frydman , hfrydman@princeton.edu, Associate ResearcherRelated Journal & Article InformationManu
Sea－Urchin－Gamete－collection
Spawning can be induced in adult sea urchinsby injecting 1 ml of a0.5M KCl solution into several sites in the soft membrane around the mouth. Within m
新加坡发布进口巴氏杀菌带壳鸡蛋的兽医条件
据新加坡食品局（SFA）消息，2019年11月1日，新加坡食品局发布进口巴氏杀菌带壳鸡蛋的兽医条件。 据通知，巴氏杀菌带壳鸡蛋只能从食品局批准的来源进口。目前，还没有海外机构获准出口这种鸡蛋至新加坡。 部分原文如下： We would like to inform that SFA has
CSF－Extract－Prep－for－Spindle－Assembly
This protocol is essentially as described by Murray (1991), Cell Cycle Extracts. In Methods in Cell Biology, B.K. Kay and B. Peng, eds. (San Diego: Ac
Inhibition－of－the－fast－and－slow－blocks－to－polyspermy
Objective: to test the effects of inhibiting the fast and slow blocks to polyspermy on sea urchin development.BackgroundSea urchin eggs release compon
A－study－of－Fetal－Alcohol－Syndrome
IntroductionFetal Alcohol Syndrome (FAS) is caused by exposure of the developing embryo to alcohol, one of several teratogenic agents which adversely
Sea－Urchin－Fertilization
Gametes are collected as described. To remove some of their jelly coat,wash eggs several times in ASW by allowing them to settle and gently pouring of
Separation－of－sea－urchin－blastomeres
Objective: to separate the Blastomere at different points in development and observe the growth of the resulting embryos.Procedure: We extractrf the g
The－Effects－of－CalciumFree－Seawater－onthe－Development－ofSea－Urchin－Embryos
ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were pl
Effect－of－UV－Treatment－on－Early－Development－of－Sea－Urchin－Embryos
Objective:To test the effects of UV radiation at 254nm on Sea Urchin embryo development after radiation at the 2-cell stage.Background:Sea Urchins exh
The－Developmental－Effects－of－Nicotine－on－Chick－Embryos
More than 22 million American women smoke and approximately 25% of these women continue to smoke during pregnancy (Lambers and Clark 1996). Cigarette
Determining－the－effects－of－nicotine－on－bone－－in－chicks
ObjectiveThe purpose of this experiment is to determine how the development of bone and cartilage in a chick are affected by treating the embryo with
Look－Ma,－No－Archenteron!－Sulfates－role－in－sea－urchin－early－development
ObjectiveTo observe the role sulfate plays in sea urchin gastrulation, and to replicate the findings of Karp and Solursh, that sea urchin embryos fail
食品类沙门氏菌检测
据澳新食品标准局（FSANZ）消息，2019年4月16日，澳新食品标准局发布召回通报，Steve's Fresh Farm Eggs正在召回可能受沙门氏菌污染的鸡蛋。受召回产品具体信息如下：受召回产品的原产国为澳大利亚。产品名称为Fresh Eggs From My Farm 12 Free
Isolating－Testes－For－IVF
In order to get the best in vitro fertilization, two criteria appear to be critical: first, selection of a mature male and second, use of L15/CS (L-15
In－vitroculture－of－early－chick－embryos
1. Use sterile technique. Prewarm Howard's Ringers in petri dish andagar/albumin culture dish to 37ºC.2. Crack 2-day egg into large sterile petri
immunofluorescence－of－rabbit－antimurine－RELMα－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of rabbit anti-murine RELMα by Peprotech.实验步骤The following protocol used B6 mice
Mouse－Spleenectomy
OUTLINEPROTOCOLGeneral anesthesia1. inject i.p. 250µl/mouse of the ANESTHETIC mixture < wait for 2-10min for the anesthetic effect>2. for verify the a
SRY基因编码的功能和结构描述
这个无内含子的基因编码一个转录因子，它是DNA结合蛋白的高迁移率族（HMG）box家族的成员这种蛋白质是睾丸决定因子（TDF），它启动男性性别决定该基因突变导致x y女性性腺发育不全（swyer综合征）；含有该基因的y染色体部分移位到x染色体可导致xx男性综合征。This intronless ge
FGFR－INHIBITOR,－SU5402,－ON－CRANIOFACIAL－DEVELOPMENT－IN－CHICKEN－EMBRYOS
ABSTRACTFibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl, FGFs are implicated in mediating epi
SRY基因突变因子与药物介绍
这个无内含子的基因编码一个转录因子，它是DNA结合蛋白的高迁移率族（HMG）box家族的成员这种蛋白质是睾丸决定因子（TDF），它启动男性性别决定该基因突变导致x y女性性腺发育不全（swyer综合征）；含有该基因的y染色体部分移位到x染色体可导致xx男性综合征。[由RefSeq提供，2008年7月
In－Situ－Hybridization－to－Somatic－Chromosomes－in－Drosophila
In Situ Hybridization to Somatic Chromosomes in DrosophilaAbby F. DernburgINTRODUCTIONIn situ hybridization was originally developed as a technique fo
Culturing－Mouse－Embryonic－Fibroblasts
MaterialsTrypsin (Gibco 25200-023)3T3 Medium: 500 mL DME (Invitrogen) + 50 mL FBS (Hyclone) + 5 mL 100x Pen/Strep2x Freezing Medium: 3T3 Medium + 20%