InVitroFertilization
When we first started using X. tropicalis, in vitro fertilization had an extremely poor efficiency. However, with the careful selection of a mature male and the use of L15/CS (L15 Leibovitch's Mediasupplemented to 10% calf serum), the in vitro fertilization efficiency has been improved drastically. Direct comparison of different medias (1xMR, L15 alone, L15/CS), clearly showed that L15/CS give......阅读全文
In－Vitro－Fertilization
When we first started using X. tropicalis, in vitro fertilization had an extremely poor efficiency. However, with the careful selection of a mature ma
Sea－Urchin－Fertilization
Gametes are collected as described. To remove some of their jelly coat,wash eggs several times in ASW by allowing them to settle and gently pouring of
The－Effects－of－Ultraviolet－Light－on－the－Fertilization
Jill K. Flemming, Franklin & Marshall College, Class of 2001IntroductionThe objective of this project is to observe the effects of UV radiation on bot
In－vitro－growth－of－seedlings
sterilisation of seeds: rinse with 70% EtOH for 30 sec put in 1% bleach (sodium hypochlorite, supplemented by a few drops of Tween-20) for 5 mi
In－vitro－Sphingomyelinase－Assay
Reagents:Lysis buffer25 mM Tris-HCl, pH 7.45 mM EDTA1 mM ATP20 µg/ml CLAP1 mM PMSFBuffer A10 mM MgCl20.2 M Tris-HCl, pH 7.40.2 % Triton X-100Buffer B0
器官培养
In vitro organ cultures (Nagy Lab)kidneylungslimb In Vitro Differentiation of ES Cells into: (Nagy Lab)Cardiac MuscleNeuronal LineagesCystic Embryoid
In－vitro－culture－of－embryonic－lungs
In vitro culture of embryonic lungsfrom Hogan LabIsolation of Lung Bud EndodermWhat you need:E11-12 mouse embryosDMEM with 5% fetal bovine serumpetri
In－Vitro－Protein－Ubiquitination－Assay
Ubiquitination is one of the most important posttranslational modifications in all eukaryote organisms. Ubiquitin-activating enzyme (E1), ubiquiti
In－Vitro－T－Cell－Activation
In Vitro T Cell ActivationIntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR).
Coating－of－Platelets－with－Antibody－in－vitro
OUTLINEAntibody-coated platelets (opsnized) may be used in the subsequent thrombophagocytosis assay. PROTOCOLResuspend 1.6x10^8 of CMFDA-labeled plate
体外重组（in－vitro－recombination）
载体与外源DNA分子体外重组时，如何选择优化连接条件以达到最高的重组率。因此有必要根据影响连接效率的因素综合考虑连接条件。影响连接效率的因素很多，如反应温度、插入片段和载体之间的摩尔比、DNA末端性质、反应时间、ATP浓度等。1. 反应温度是比较重要的影响因素。因为连接酶的最适反应温度为37℃，
InVitro－Adipocytes－Differentiation
IntroductionObesity is a significant clinical problem that contributes to life-threatening diseases such as diabetes and atherosclerosis. With an incr
In－Vitro－Conservation－and－Cryopreservation－of－Ornamental－Plants
Today, the conservation of ornamental germplasm can take advantage of innovative techniques which allow preservation in vitro (slow growth storage
Invitro－Phagocytosis－Assay－of－Macrophages
IntroductionThe term phagocytosis itself describes its mean phage = engulfment; cytosis: cell process. In other words, phagocytosis is the cellular pr
Isolating－Testes－For－IVF
In order to get the best in vitro fertilization, two criteria appear to be critical: first, selection of a mature male and second, use of L15/CS (L-15
Preparation－of－Luciferin－for－In－Vitro－and－In－Vivo－Bioluminescent－Assays
Preparation of Luciferin forIn Vitro Bioluminescent AssaysMaterials• D-Luciferin Firefly, potassium salt, 1.0 g /vial(Caliper Life Sciences Part Numbe
Preparation－of－Luciferin－for－In－Vitro－and－In－Vivo－Bioluminescent－Assays
实验概要Reagent for immunoassay, ligand binding assay and ligand receptor assay in which luciferin is covalently bonded to a molecule having biological
In－vitro－Assessment－of－Metabolic－－in－Suspension－Cryopreserved－Hepatocytes
实验概要BackgroundThe pharmaceutical and biotechnology industry’s goal is to discover therapeutic agents that are both safe and effective at treating or
Preparation－of－Luciferin－for－In－Vitro－and－In－Vivo－Bioluminescent－Assays
实验概要Reagent for immunoassay, ligand binding assay and ligand receptor assay in which luciferin is covalently bonded to a molecule having biological
早期胚胎发育中的单胚胎细胞基因表达（二）
“We picked 42 genes to validate on the BioMark system,” Dr. Yao said. “We picked them to represent different functional categories.”“We used the F
发育生物学
In Vitro Production of Bovine Embryos (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)This protocol describe procedures for in vitro
A－novel－in－vitro－3dimensional－angiogenesis－model
1. Human microvascular endothelial cells (HMVECs) with primary cell kits were cultured on collagen type I-coated dishes to 80% confluency, then ov
Betagal－staining－of－eukaryotic－cells－in－vitro
(Modification of methods of Dr. Seong-Seng Tan and Promega's "Protocols and Applications Guide")Cells previously transfected with a lac Z construc
Keynote－Speaker:－Xiaoliang－Xie
Xiaoliang Xie Professor Xie is an internationally renowned biophysical chemist, and the Lee Shao-kee professor of Peking University. After a career a
In－Vitro－prostate－colony－and－sphereforming－assays
1. Prostates were dissected, minced into small pieces with a steel blade, and digested with 0.8 mg/ml collagenase in 10 ml of primary cell medium/
研究发现评估IVF中精子质量的小RNA分子标志物
4月9日，国际学术期刊Cell Discovery 在线发表了中国科学院生物化学与细胞生物学研究所吴立刚研究组、上海市计划生育科学研究所施惠娟实验室和复旦大学附属妇产科医院上海集爱遗传与不育诊疗中心陈国武实验室的合作研究成果“Identification of small noncoding R
Cell－Discovery：评估IVF中精子质量的小RNA分子标志物
国际学术期刊Cell Discovery在线发表了中国科学院生物化学与细胞生物学研究所吴立刚研究组、上海市计划生育科学研究所施惠娟实验室和复旦大学附属妇产科医院上海集爱遗传与不育诊疗中心陈国武实验室的合作研究成果“Identification of small noncoding RNAs as
In－Vitro－Production－of－Sweet－Peas－(Lathyrus－odoratus－L.)－via－Axillary－Shoot
The genus Lathyrus is best known because it includes a number of wild relatives of the protein pea which, despite being generally neglected and un
慢病毒用于体外（in－vitro）实验：感染培养原代细胞和...
慢病毒用于体外（in vitro）实验：感染培养原代细胞和建系细胞1. 慢病毒对各种细胞和组织的亲嗜性不同，用户使用Invabio提供的慢病毒之前可以通过查阅相关文献，了解慢病毒对您的目的细胞的亲嗜性，感染复数（MOI 值）以及在体内（in vivo）注射所需要的病毒量。如果没有相关文献支持
Illumina与QIAGEN合作提供基于测序的InVitro－Diagnostic－(IVD)试验
分析测试百科网讯 近日，Illumina（纳斯达克：ILMN）和QIAGEN N.V.（纽约证券交易所：QGEN）（法兰克福主要标准：QIA）宣布了一项为期15年的合作计划，旨在扩大基于NGS的IVD试剂盒的可用性和使用范围，包括用于患者管理的伴侣诊断。合作加速下一代测序（next-generati