ImmatureEmbryoRescueandCulture
Embryo culture techniques have many significant applications in plant breeding, as well as basic studies in physiology and biochemistry. Immature embryo rescue and culture is a particularly attractive technique for recovering plants from sexual crosses where the majority of embryos cannot survive in vivo or become dormant for long periods of time. Overcoming embryo inviability is the most common reason for ......阅读全文
Cultex细胞暴露技术建立呼吸道过敏反应的人肺体外模型－1
方法简介化学品引起的呼吸道过敏反应，不论是体内还是体外，目前尚缺少有效的评价方法。因此，新实验方法的开发收到极大的关注，特别是考虑到REACH（《化学品注册、评估、许可和限制》）新的法规规定。应注意的是，REACH指导方针明确指出，应尽量避免动物实验，其中，化妆品材料应在2013年全面禁止动物实验。
Sea－Urchin－Gamete－collection
Spawning can be induced in adult sea urchinsby injecting 1 ml of a0.5M KCl solution into several sites in the soft membrane around the mouth. Within m
A－Yeast－Secretion－Trap－Assay－for－Identification－of－Secreted－Proteins－...
Secreted proteins from plants and phytopathogens play important roles in their interactions and contribute to elaborate mechanisms of attack, defe
The－Effects－of－NiCl2on－Spicule－Formation
The Effects of NiCl2on Spicule FormationJessica Ann Billet, Franklin and Marshall, Class of 2000Background and ObjectiveSea urchins exhibit radial hol
DEVELOPMENTAL－EFFECTS－OF－LITHIUM－CHLORIDE－ON－XENOPUS－EMBRYOS
PURPOSE: The objective of this experiment is to study the effect of lithium chloride treatment on early blastula xenopus embryos.INTRODUCTION: When th
Axolotl－Microsurgery
I. Embryo Preparation1) Manually dejelly embryo of appropriate stage. Use fine foreps to break hole in hard jelly coat. Remove membrane around embryo
Examination－of－a－Mammalian－Blood－Smear
Examination of a Mammalian Blood SmearThe distribution and appearance of the formed elements of blood can tell a great deal about the condition of the
Sectioning－stained－embryos.
The protocols for plastic and wax sections as used by the Vize lab. These protocols work, but they have not been optimized. If anyone has better proto
小鼠feeder细胞分离
You will need:13.5 day pregnant mouse (we use MTK NEO inbred white mice)2 sets sterile instrumentsone containing a pair of curved forceps and a pair o
Sea－Urchin－staging－series
Invertebrate embryos develop at different rates depending on the temperature of incubation. Descriptive stages based on the number of cells and their
The－Effects－of－CalciumFree－Seawater－onthe－Development－ofSea－Urchin－Embryos
ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were pl
我国科学家开发出一种新型RNA编辑系统
使用工程核酸酶的基因组编辑技术，比如锌指核酸酶（ZFN）、转录激活因子样效应物核酸酶（TALEN）和CRISPR系统中的Cas蛋白已被用于操纵许多有机体的基因组。最近，科学家们已将胞苷脱氨酶或腺苷脱氨酶与CRISPR-Cas9融合在一起，构建出可编程的DNA碱基编辑器，从而为校正致病性突变提供新
斑马鱼平台助力HSP发病机理研究
遗传性痉挛性截瘫（HSP）又称家族性痉挛性截瘫，是一种神经系统退行性变性疾病。其病理改变主要是脊髓中双侧皮质脊髓束的轴索变性或脱髓鞘，以胸段最重。 临床表现为双下肢肌张力增高，腱反射活跃亢进，病理反射阳性，呈剪刀步态。2018年5月11日，中国国家卫生健康委员会等5部门联合制定了《第一批罕见病目录》
多功能胚胎激光破膜仪（一）
Laser-assisted derivation of human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis.T. Turetsky, E. Aizenman, Y. G
Science：震撼视频！哈佛科学家揭示“胚胎发育”最详细过程
一个受精卵究竟是如何产生构成完整身体的多种细胞类型、组织和器官的？这是生物学领域最大的谜题之一。如今，结合单细胞测序技术和新型计算工具，来自哈佛大学、Broad研究所等机构的科学家们提供了关于这一过程最详细的图片。4月26日，Science杂志用3篇论文报道了这一突破性成果。为了追踪数千个细胞及其后
成熟胚培养的技术特点
中文名称成熟胚培养英文名称culture of mature embryo定 义将子叶期以后的胚从母体上分离出来，放在无菌的人工环境条件下使其进一步生长发育形成幼苗的技术。应用学科细胞生物学（一级学科），细胞培养与细胞工程（二级学科）
幼胚培养的技术特点
中文名称幼胚培养英文名称culture of larva embryo定 义将子叶期以前的具胚结构的幼小胚从母体上分离出来，放在无菌的人工环境条件下使其进一步生长发育形成幼苗的技术。应用学科细胞生物学（一级学科），细胞培养与细胞工程（二级学科）
Expression－Library－Screening－(Procaryotic)－Using－APFusion－Proteins
Outline:Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after e
纯化鸡胚细胞疫苗的功能特点
中文名称纯化鸡胚细胞疫苗英文名称purified chick embryo cell vaccine;PCEC定 义取鸡成纤维细胞原代培养的狂犬病毒，经β-丙内酯灭活而制成的一类用于接触前或接触后预防狂犬病的冻干制剂。应用学科免疫学（一级学科），应用免疫（二级学科），免疫预防（三级学科）
纯化鸡胚细胞疫苗的概念和功能
中文名称纯化鸡胚细胞疫苗英文名称purified chick embryo cell vaccine;PCEC定 义取鸡成纤维细胞原代培养的狂犬病毒，经β-丙内酯灭活而制成的一类用于接触前或接触后预防狂犬病的冻干制剂。应用学科免疫学（一级学科），应用免疫（二级学科），免疫预防（三级学科）
纯化鸡胚细胞疫苗的定义
中文名称纯化鸡胚细胞疫苗英文名称purified chick embryo cell vaccine;PCEC定 义取鸡成纤维细胞原代培养的狂犬病毒，经β-丙内酯灭活而制成的一类用于接触前或接触后预防狂犬病的冻干制剂。应用学科免疫学（一级学科），应用免疫（二级学科），免疫预防（三级学科）
转基因
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Core)Thi
Cell－death－detection－in－Xenopus－embryos－by－ELISA
Sample preparationWash embryos 1 x in 25% MMRRemove excess bufferAdd 10 volumes "incubation buffer", i.e. 50 µl for 5 embryosLyse the embryos by gentl
FGFR－INHIBITOR,－SU5402,－ON－CRANIOFACIAL－DEVELOPMENT－IN－CHICKEN－EMBRYOS
ABSTRACTFibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl, FGFs are implicated in mediating epi
组织学——组织切片
· Histological techniques (William H. Heidcamp)Very detailed guide to histological techniques, like fixation, dehydration, embedment and subs
TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go high
Tunel－Procedure－in－Bovine－Embryos－牛胚胎TUNEL检测凋亡
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go highe
TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go high
FISH－protocols－for－Drosophila2
3. Methods3.1 RNA Probe Preparation1. Different strategies can be used to prepare template DNA for synthesizing antisense RNA probes by in vitro tr
Cytokine－induced－angiogenesis－in－chick－embryos.
Objective:The purpose of this experiment was to explore the effects of the growth factors bFGF and VEGF on blood vessel formation within the chorioall