WHITEPAPER:VirotherapyProcessOptimization(一)
WHITE PAPER: Virotherapy Process Optimization采用一系列方法将病毒工程开发用于疾病治疗已经成为一种新型的应用,该应用广泛的定义为病毒疗法,包括用于基因治疗的病毒载体(Figure 1)和具有溶瘤细胞功能的病毒(Figure 2)。虽然这些产品大部分都处在临床开发阶段,但是药物作用靶点的多样性以及未来机会的财富都是令人鼓舞的。病毒疗法是一门以病毒为基础的技术,一个重要的挑战就是在任何的时间点包括早期的研发,产品的生产,产品的包装上市都能清楚地了解样品的质量和性能,其中最重要的一点就是病毒的定量。在过去,病毒的定量依赖于噬斑法和电镜等方法,这些方法需要大量的劳动力,等待很长时间才能获得结果,且获得的结果具有很大的主观性。然而,以新的病毒学技术的多样性为基础的病毒药物和疫苗需要新的分析方法。在这篇文章中,我们将讨论病毒计数仪3100实时的病毒浓度监测功能如何能显著的促进病毒疗法产品的......阅读全文
WHITE-PAPER:-Virotherapy-Process-Optimization(一)
WHITE PAPER: Virotherapy Process Optimization采用一系列方法将病毒工程开发用于疾病治疗已经成为一种新型的应用,该应用广泛的定义为病毒疗法,包括用于基因治疗的病毒载体(Figure 1)和具有溶瘤细胞功能的病毒(Figure 2)。虽然这些产品大部分
WHITE-PAPER:-Virotherapy-Process-Optimization(二)
Figure 3. Virus Counter 3100 (top) and Autosampler (bottom)Proof of Principle病毒计数仪已经被广泛应用于多种病毒的计数,包括基因传递载体和溶瘤功能的病毒。这里我们列出一些检测的数据,证实病毒计数仪快速的病毒定量在病毒疗法领域
New-white-paper-explains-how-to-increase-brewing-process-……
Ensuring production processes will operate as necessary and generate the desired product quality and yield in the required time is central to succ
White-paper:-Don`t-Get-Burned-in-Combustion
Control of combustion processes requires reliable oxygen (O2) and carbon monoxide (CO) measurement and control to ensure safety, maximize efficien
New-white-paper-explains-how-ISM-measurement-systems-can-reduce-the-SOP
Creating and managing Standard Operating Procedures (SOPs) to meet quality control system requirements, necessitates a great deal of pharmaceutica
Ligation-Optimization
The following protocol can be used to optimize ligation conditions for difficult to clone (e.g. very large) fragments. The principle is to independent
Beginners-Guide:-Validat,-Verificat-and-Monitoring-of-Inspection-Equipment
February 28, 2017 -Validation, verification and routine performance monitoring are often used interchangeably, creating confusion within organizat
Product-Inspection-Equipment-Can-Boost-OEE-in-the-Pharmaceutical-Industry
March 29, 2017 -Overall equipment effectiveness (OEE) scores tend to be lower in the pharmaceutical industry compared to other industries. This is
International-Process-Analytics-and-Control-Congress
International Process Analysis and Control Congress, China 2012IPAC 2012 Call for paper and Introduction The International Process Analysis & Control
FTNIR-Process-Monitoring
FT-NIR Process MonitoringToday many manufacturers are striving not only to produce the highest quality final product but also to improve manufactu
Detection-of-apoptotic-process-in-situ-using-immunocytochemical
1. INTRODUCTION Apoptosis was observed from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pathological
IPAC-2012-Call-for-paper-and-Introduction
IPAC 2012 Call for paper and Introduction The International Process Analysis & Control Congress, China 2012 (IPAC 2012) is the first event spons
Transformation-of-Electrocompetent-E.-coli-with-Blue/White-selection
Desalt DNA template by EtOH precipitation in NaOAc followed by at least 2x washes with 70% EtOH. Resuspend in 5 - 15 µL of sterile H2O.Rinse cuvettes
-Process-Instruments公司推出一种检测烯烃含量的新技术
Process Instruments公司推出一种检测煤气或其它燃料中烯烃含量的新技术 检测烯烃在汽油或其他燃料中含量的方法已经有了新的突破。目前ASTM标准里检测烯烃含量的方法只包括荧光指示剂方法和超临界流体色谱法,但是这两种测量方法既不准确也费时。拉曼光谱非常适用于烯烃的检
Leister-Process-Tech.收购IR-Microsystems
Leister Process Technologies宣布收购瑞士传感器公司IR Microsystems SA,该公司是用于气体检测单元的二极管激发器的优秀供应商。此次收购在Leister公司长期发展策略上是一个重要的里程碑,公司的长期发展战略集中在传感器、光学和激光技术。 “以IR M
层析方法:纸层析(paper-chrmatography)
纸层析是分配层析法的一种,其以滤纸为惰性载体,展层溶剂大多是由水饱和的有机溶剂组成。滤纸纤维的—OH 基的亲水基团可吸附有机溶剂中的水作固定相,有机溶剂作流动相,当流动相沿滤纸自下向上展开,称为上行层析;反之,流动相自上向下移动,为下行层析。纸层析法主要依据混合组分对两相分配系数的差异而进行分离的,
射频混频器设计Harmonic-balance-simulation-speeds-RF-mixer-design2
Figure 3. Performance of a somewhat idealized Marchand balun with Z0o = 25 ohms, Z0e = 180 ohms, and ZL = 60 ohmsThe output terminals are each treat
Detection-of-apoptotic-process-in-situ-using-immunocytochemical2
B) TUNEL in situ procedureB.2.1 Materialsproteinase K (pK) (A2), H2O2 , TdT buffer (A1), TdT enzyme (A2), biotinylated dUTP (A2), TB buffer (A1), seru
科研人的“恋爱脑”:结婚一周年,送一篇“paper”
原文地址:http://news.sciencenet.cn/htmlnews/2024/3/519796.shtm 学霸如何表达爱? 今日主角团,是学霸也是爱神,爱科学也会科学爱:他们可以把论文(paper)作为
Protease-assay
In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to softe
New-Process-Chemistry-Technology-Allows-Automated-Lab-Reactor-Control
The new METTLER TOLEDO RX-10 Reactor Control connects and automates jacketed lab reactors and controls thermostats, stirrers and pumps with a single t
IPAC-2012-Conference-Background
International Process Analytics and Control Congress, China 2012 The International Process Analysis & Control Congress, China 2012 (IPAC 2012) is
实验室自动化与筛选协会2013亚洲会展展商技术论坛
第一天(2013年6月6日)13:45-14:30 展商技术论坛 1: 会场:宴宾厅 II,2楼 议题:Cell-based assays and 3D cell culture:Your body is in 3D – Why is your cell cult
氨基酸的纸层析(paper-chrmatography)
一、 实验目的1.了解并掌握氨基酸纸层析的原理和方法。2.学习纸层析法的操作技术,分析未知样品氨基酸的成分。二、 实验原理用滤纸为支持物进行层析的方法,称为纸层析法,它是分配层析法的一种。纸层析所用的展层溶剂大多是由水饱和的有机溶剂组成。滤纸纤维的—OH 基的亲水性基团,可吸附有机溶剂中的水作为
Examination-of-a-Mammalian-Blood-Smear
Examination of a Mammalian Blood SmearThe distribution and appearance of the formed elements of blood can tell a great deal about the condition of the
Protease-assay
实验概要 In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in
SQ-Blood-DNA-Midi-Protocol-for-500l3ml-whole-blood
实验概要The E.Z.N.A.® SQ Blood DNA Kit is designed for isolating high molecular weight genomic DNA from fresh, frozen or anticoagulated whole blood. The
SQ-Blood-DNA-Maxi-Protocol-for-410-ml-whole-blood
实验概要The E.Z.N.A.® SQ Blood DNA Kit is designed for isolating high molecular weight genomic DNA from fresh, frozen or anticoagulated whole blood. The
转氨基作用(纸层析法,Paper-Chromatography)
一.目的 了解动物组织的转氨基作用,学习转氨基反应产物的纸层析鉴定方法。 二.原理 氨基酸在体内分解代谢的主要方式是脱氨基作用。脱氨基作用的主要方式有氧化脱氨基、转氨基、联合脱氨基及非氧化脱氨基。转氨基是指在转氨酶的催化下,氨基酸的 α-氨基与α-酮酸的α酮基的互换反应。转氨酶广泛分
Interview-CEO-of-H.E.L:-Make-a-Healthier,-more-Sustainable,-and-Safer-world
How to carry out process safety control? How to scale up the production scale from a 500 ml laboratory benchtop to a 10000 m3 factory? How to optimize