Isolationofperipheralbloodmononuclearcells(PBMC)
1. Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2. PBMC were isolated by centrifugation over lymphocyte separation medium. 3. After three washes in phosphate-buffered saline (PBS), the PBMC were counted and cryopreserved in 90% fetal bovine serum and 10% dimethyl sulfoxide at 107 cells/ml. 4. Thawed PBMC were mitogenically stimulated in the following medium: Dulbe......阅读全文
Isolation of peripheral blood mononuclear cells (PBMC)
1. Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2. PBMC were isolated by centrifugation over lymphocyte separation medium. 3
Human Peripheral Blood Mononuclear Cell Preparation
This protocol describes a procedure for isolating human peripheral blood mononuclear cells (lymphocytes and monocytes) from a Buffy Coat (obtained fr
Peripheral blood “endothelial progenitor cells”
EPC Isolation and Characterization1. EPCs were obtained by isolating mononuclear cells using Ficoll density-gradient centrifugation of human blood buf
FACS Analysis Using Peripheral Blood Cells
FACS Analysis Using Peripheral Blood Cells Collect blood (75 microliters) into 1ml PBS containing 5 microM EDTA (10 microliters of 0.5 M stock) a
Preparation Of Peripheral Blood Cells For Chromosome Analysis
实验概要 Lymphocytes are differentiated cells which normally do not undergo subsequent cell divisions. By culturing lymphocytes in the presence of a m
Harvesting lymphocytes from Peripheral Blood
Blood Harvest· Anesthetize mice with 50ml of Xylene-Ketamine(2:1) coctail· Pin animal to a board (use distal extremities) supine·
PBMC细胞的精确计数和活性分析（一）
一、PBMC细胞计数的现状1. 目前，绝大多数的科研工作者仍在使用血球计数板在显微镜下进行手工计数PBMCs；2. 但是显微镜下由于白细胞和红细胞大小非常接近，不能完全区分白细胞和红细胞；3. 通过红细胞的双凹形形态鉴别红细胞，需要经验丰富的操作者，且不停地调焦来鉴别；可想而知要把每个红细胞鉴别出来
Purification of human mononuclear cells and neutrophils
PurposeMaterials10ml 6% dextran + 7ml citrate/citric acidDextran: T500 --> 6g+100ml PBSCitrate solution: 25g Na Citrate + 8g citric acid + 500 ml PBS4
红细胞裂解的实验方法步骤
IntroductionPrior to using lymphoid tissue cell suspensions for flow cytometric analysis and/or for in vitro functional assays, it is recommended to r
Culture of Peripheral Blood Lymphocytes for Chromosome Analysis
实验概要 Provide information about chromosomal abnormalities. 实验原理 The blood cell karyotyping method was developed to provide information about chr