Isolationofnormalmammaryepithelialcells
1. A small piece of tissue from each specimen was removed and minced. 2. The tissue was digested with collagenase overnight. 3. To remove the collagenase and the majority of the stromal cells, the tissue was rinsed and centrifuged. 4. The tissue was inoculated into a 60-mm tissue culture dish coated with collagen type I and grown in primary cell system supplemented with growth factors and ho......阅读全文
Isolation－of－normal－mammary－epithelial－cells
1. A small piece of tissue from each specimen was removed and minced. 2. The tissue was digested with collagenase overnight. 3. To remove the collagen
Isolation－of－Human－Pulmonary－Epithelial－Cells
Isolation of Human Pulmonary Epithelial Cells 1. Pulmonary epithelial cells from human lung tissue were isolated.2. Lungs were perfused with 10
Isolation－of－human－endometrial－epithelial－cells
Tissue collection1. Endometrial biopsies were collected from women undergoing gynaecological procedures for benign conditions. 2. All women reported
Isolation－of－human－prostatic－epithelial－cells
1. A small piece of tissue from each specimen was removed and minced. 2. The tissue was digested with collagenase overnight. 3. To remove the collagen
Isolation－of－human－colonic－epithelial－cells－(EC)
1.Human colonic epithelial cells (EC) were isolated from freshly resected colonic surgical specimens obtained. 2.All diagnoses were confirmed by clini
Isolation－of－human－primary－ovarian－surface－epithelial－cells
1. Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institutional Review (References)
Isolation－of－human－primary－gastric－mucosa－epithelial－cells
1. A piece of gastric mucosa (∼3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2. The patients were proven to be no H.
Isolation－of－retinal－pigment－epithelial－cell（一）
The pigmented layer of retina or retinal pigment epithelium (RPE) is the pigmented cell layer just outside the neurosensory retina that nourishes
Isolation－of－retinal－pigment－epithelial－cell（二）
15. This medium is replaced after 1 day with 5% serum-containing RPE medium, and subsequent changes were made every 2 to 3 days.16. After 3 to 4 weeks
Isolation－of－papillary－cells
实验概要This protocols provides a general protocol for isolation of papillary cells.实验步骤Isolation of renal papillary cells1. For isolation of papillary c
Isolation－of－papillary－cells
Isolation of renal papillary cells1. For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/
Isolation－of－lymphatic－endothelial－cells
Dermal Cell Suspensions 1. Dermatomed 0.8-mm split-thickness skin was obtained from adult healthy individuals undergoing elective surgery. 2
Isolation－of－rodent－pancreatic－β－cells
1. Adult rats weighing 250-350g were anesthetized, sacrificed and immediately used for pancreas sampling.2. Rat islets were isolated from male wistar
Isolation－of－lymphatic－endothelial－cells
实验概要This protocols provides a general protocol for isolation of lymphatic endothelial cells.实验步骤Dermal Cell Suspensions1. Dermatomed 0.8-mm split-thic
Stem－cell－characteristics－of－amniotic－epithelial－(AE)－cells
Isolation of AE Cells1. Human placentae were obtained with the approval of the institutional review board, after uncomplicated elective caesarean
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous SystemScott R. Hutton and Larysa H. Pevny1UNC Neuroscience Center
Isolation－and－culture－of－porcine－muller－cells
1. Porcine eyes were harvested from pentobarbital-anesthetized animals, each weighing 30 to 40 pounds, and transported to the laboratory in ice-co
Isolation－of－human－atrial－or－ventricular－cells
1. Human tissue was derived from atrial or ventricular biopsy specimens belonging to patients undergoing heart surgery.2. Isolated myocardial tissue w
Isolation－and－culture－of－pancreatic－stellate－cells
Pancreatic stellate cells (PaSCs or PSCs) are myofibroblast-like cells found in the areas of the pancreas that have exocrine function. PaSCs are
PriCells:－Isolation－of－endothelial－progenitor－cells－(EPCs)
PriCells: Isolation of endothelial progenitor cells (EPCs) 1. Twenty-four mL venous blood was collected at each time point into Vacutainer CPT Mono
Isolation－of－peripheral－blood－mononuclear－cells－(PBMC)
1. Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2. PBMC were isolated by centrifugation over lymphocyte separation medium. 3
Isolation－of－human－prostatic－smooth－muscle－cells
Human prostate tissues1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of the prostate. 2.All
Isolation－and－characterization－of－rat－glomerular－endothelial－cells
Glomerular endothelial cells (GECs) from the kidney are in close juxtaposition to other cell types, such as mesangial cells, and may respond to as
Isolation－of－human－corneal－endothelial－cells－(HCECs)
Isolation of HCECs1. The corneoscleral tissues were rinsed three times with primary cell culture system containing 50 mg/mL gentamicin and 1.25 mg
Isolation－of－human－uterine－smooth－muscle－cells
1. Approval to utilize human tissue was given by Hospital's Committee on Research Involving Human Subjects. 2. Human myometrial and leiomyoma tiss
Isolation－and－cultivation－of－endothelial－progenitor－cells－(EPCs)
Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue regeneration and substantially contri
Isolation－and－Culture－of－Human－Liver－Stem－Cells
Materials and Methods1. Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy liver tissue (
Primary－cultures－of－intrahepatic－bile－duct－epithelial－cells－isolated－and...
Primary cultures of intrahepatic bile duct epithelial cells isolated and cultured1. Liver was surgically removed and perfused via the hepatic vein.2.
与肾癌相关的AREG基因编码功能描述
这个基因编码的蛋白质是表皮生长因子家族的一员。它是一种自分泌生长因子，也是星形细胞、雪旺细胞和成纤维细胞的有丝分裂原。与表皮生长因子（egf）和转化生长因子α（tgfα）有关。该蛋白与EGF/TGFα受体相互作用，促进正常上皮细胞的生长，抑制某些侵袭性癌细胞系的生长。在乳腺、卵母细胞和骨组织发育中也
AREG基因突变与药物因子介绍
这个基因编码的蛋白质是表皮生长因子家族的一员。它是一种自分泌生长因子，也是星形细胞、雪旺细胞和成纤维细胞的有丝分裂原。与表皮生长因子（egf）和转化生长因子α（tgfα）有关。该蛋白与EGF/TGFα受体相互作用，促进正常上皮细胞的生长，抑制某些侵袭性癌细胞系的生长。在乳腺、卵母细胞和骨组织发育中也