Placentaltrophoblastandchorioniccellcultures
1. Placental trophoblast and chorionic trophoblast cells were prepared using a modification of the method. 2. Term human placentae and chorion tissue were obtained from normal women after elective section at term. 3. Several aliquots of tissue were removed randomly from the maternal side of the placenta, pooled, and digested with 0.125% trypsin and 0.02% deoxyribonuclease-I in primary cell cultur......阅读全文
Isolation－of－normal－mammary－epithelial－cells
1. A small piece of tissue from each specimen was removed and minced. 2. The tissue was digested with collagenase overnight. 3. To remove the collagen
Isolation－of－human－prostatic－epithelial－cells
1. A small piece of tissue from each specimen was removed and minced. 2. The tissue was digested with collagenase overnight. 3. To remove the collagen
Cryopreservation－of－Cell－Lines
AimThe protocol below describes the use of passive methods involving an electric -80ºC freezer for the cryopreservation of cell cultures. ECACC routin
Activation－and－Expansion－of－Human－Treg－Cells
实验概要This protocol is intended for activation and expansion of human Treg cells isolated with the Dynal® CD4 CD25 Treg Kit (Cat. no. 113.23D). The exp
The－OP9DL1－System:－Generation－of－TLymphocytes－from－Embryonic4
TROUBLESHOOTINGProblem: The OP9 cells are more than 80%-90% confluent.Solution: It is important when creating working stocks of OP9 cells for freezing
Primary－Culture－of－Pulmonary－Arterial－Smooth－Muscle－Cells
Primary Culture of Pulmonary Arterial Smooth Muscle Cells 1.Primary cultures of Pulmonary Arterial Smooth Muscle Cells (PASMCs) were isolated from hum
细胞团介绍
内细胞团（英语：Inner cell mass，缩写：ICM）是大多数真兽类哺乳动物在胚胎发生中的一个早期阶段，又称胚细胞（embryoblast）。是一团位于初期胚胎中的一个细胞团块，也是最后将会发育成为胎儿的部位。 在科学研究上，内细胞团是早期干细胞的来源之一，可以形成除了滋养母细胞（tr
内细胞团的概念
内细胞团（英语：Inner cell mass，缩写：ICM）是大多数真兽类哺乳动物在胚胎发生中的一个早期阶段，又称胚细胞（embryoblast）。是一团位于初期胚胎中的一个细胞团块，也是最后将会发育成为胎儿的部位。在科学研究上，内细胞团是早期干细胞的来源之一，可以形成除了滋养母细胞（tropho
胚胎干细胞的基本信息
胚胎干细胞（Embryonic stem cell，简称ESC）是一类具有多能性的干细胞。在卵细胞受精后，受精卵经过桑葚胚阶段，进入囊胚阶段。囊胚中的细胞可以归入两个大类：滋养层（trophoblast，TE）和内细胞群（inner cell mass，ICM）。滋养层的细胞会分化为胚胎外的组织（胎
Resuscitation－of－Frozen－Cell－Lines
AimMany cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use them the cells must be thawed and put into
Testing－for－Mycoplasma－by－Indirect－DNA－Stain－(Hoechst－33258－stain)
AimDNA staining methods such as Hoechst staining techniques are quick with results available within 24 hours, which compares favorably with 4 weeks fo
Trabecular－cell－monolayer－culture
Originally described in 1979 and more recently modified by Stamer et al primary trabecular monolayer cell culture has been a cornerstone for inves
Culture－of－Endometrial－Explants
Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion of Proteins and ProstaglandinsP. J. Hansen and J.
Transformation－of－E.－coli－by－Electroporation
实验概要 Electrocompetent bacteria are prepared by growing cultures to mid-log phase, washing the bacteria extensively at low temperature, and
Tissue－Culture－Methods2
IV. MAINTENANCECultures should be examined daily, observing the morphology, the color of the medium and the density of the cells. A tissue culture log
Culture－of－Endometrial－Explants－to－Monitor－Synthesis
Reprinted from:Hansen, P.J and Betts, J.G. (1992) Culture of endometrial explants and peri-implantation conceptuses to monitor synthesis and secretion
Exercise－12.10－－Establishment－of－a－Primary－Culture
Exercise 12.10 - Establishment of a Primary CultureLEVEL IIIMaterialsChick embryo (approximately 8 days old)70% (v/v) ethanol for swabbingSterile scis
DIRECT－AND－SHORTTERM－PROCEDURE－FOR－HARVESTING－BONE－MARROW－CHROMOSOMES
I. PurposeTo identify chromosome anomalies in hematopoietic cells. Used especially for chromosome studies for hematological disorders such as preleuke
Isolation－of－rat－cardiac－fibroblasts－and－cardiomyocytes
1. Hearts were removed from newborn rats (day 0), put into calcium- and bicarbonate-free HEPES-buffered Hanks’ medium, cut into pieces and digeste
Culturing－Rat－Fetal－Neural－Stem－Cells
实验概要Rat neural stem cells (NSCs) serve as a well-established model for investigating human brain development, disease processes, and treatment stra
Subculture－of－Suspension－Cell－Lines
AimIn general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transform
Different－types－of－human－cells
Human primary cells are cells taken from living human beings and cultured. These cells retain the differentiation of the original cells taken in
Fibroblast－Cell－Systems1
THESE INSTRUCTIONS APPLY TO ORDERS CONTAINING THE FOLLOWING CELL PRODUCTSCryopreserved Cells (Single donor)CC-2511NHDF -Ad3 500,000 cells/cryovialCC-2
Isolation－and－culture－of－SpragueDawley－rat－aortic－smooth－muscle－cells
The intact mature arterial media is composed of at least four phenotypically unique cell subpopulations that reside in distinct medial layers. Th
BAC－DNA分离方法－Isolation－of－BAC－DNA－from－Largescale－Cultures
Isolation of BAC DNA from Large-scale CulturesJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid W. Russe
Primary－brain－cell－isolation－and－culture
1. Cerebella were removed from 7-day-old mice and passed through Nitex nylon netting (80 μm pore size) into primary cell system containing 20% (v/
Isolation－of－human－uterine－smooth－muscle－cells
1. Approval to utilize human tissue was given by Hospital's Committee on Research Involving Human Subjects. 2. Human myometrial and leiomyoma tiss
胚泡的结构及功能
哺乳动物特有的囊胚。桑椹胚细胞继续分裂增生，卵裂细胞的分泌导致细胞团间出现裂隙，后扩大成囊腔，称胚泡腔或囊胚腔，腔内充满液体。细胞分为二部分，构成胚泡外壁的扁平细胞层，称滋养层（trophoblast），可以从母体吸取营养，此层将形成胎盘的一部分。另一部分细胞成团在胚泡腔的一侧附着在滋养层上，称内细
Identification－and－expansion－of－the－tumorigenic－lung－cancer－stem－cell－...
Identification and expansion of the tumorigenic lung cancer stem cell populationLung cancer contains a rare population of CD133+ cancer stem-like ce
Testing－for－Bacteria－and－Fungi
AimIn cases of gross contamination the naked eye may identify the presence of bacteria and fungi. However, it is necessary to detect low-level infecti