Intestinalintraepitheliallymphocytes

Intestinal intraepithelial lymphocytes Intestinal intraepithelial lymphocytes (IELs) are mostly T cells, which are continuously associated with gut epithelium.1. The epithelial integrity is disrupted and IEL and intestinal epithelial cell (IEC) are liberated into the medium without altering the basement membrane. 2. The original protocols developed for IEL isolation require diss......阅读全文

Intestinal-intraepithelial-lymphocytes

Intestinal intraepithelial lymphocytes    Intestinal intraepithelial lymphocytes (IELs) are mostly T cells, which are continuously associated with gut

Adhesion-and-Diapedesis-of-Lymphocytes

Cell adhesion is a fundamental feature of multicellular organisms including their defense mechanisms. In the later case in mammals, leukocytes play ce

Isolation-of-liver-infiltrating-lymphocytes

1. Hepatic T cells were isolated from fresh liver tissue collected into primary cell system.2. In the second method, tissue was diced using sterile bl

肠道共生病毒维持肠道粘膜免疫稳态的作用和机制

  近日,中国科学技术大学基础医学院、中科院天然免疫与慢性疾病重点实验室和合肥微尺度物质科学国家研究中心教授周荣斌、江维、朱书课题组合作在《自然-免疫学》(Nature Immunology)发表了题为Commensal viruses maintain intestinal intraepithe

Harvesting-lymphocytes-from-Peripheral-Blood

Blood Harvest·        Anesthetize mice with 50ml of Xylene-Ketamine(2:1) coctail·        Pin animal to a board (use distal extremities) supine·       

MN-in-Human-Lymphocytes-(method-description)

MN in Human Lymphocytes (method description)Lymphocyte isolation    Lymphocytes were isolated using Ficoll-Paque density gradients. Blood was diluted

Culture-of-Peripheral-Blood-Lymphocytes-for-Chromosome-Analysis

实验概要Provide information about chromosomal abnormalities.实验原理The  blood cell karyotyping method was developed to provide information  about chromosomal

LCMSMS-Analysis-of-AntiHIV-Agents-in-Lymphocytes

这是Gilead的一个做HIV药的报告(英文),我觉得比较有意义,主要是怎么设置LC/MS/MS的方法,其中,提到了切盐,定量等。 LCMSMS Analysis of Anti-HIV Agents in Lymphocytes    

Neuropeptides-VIP-and-PACAP-inhibit-the-apoptosis-of-activated-T-cells

Vasoactive intestinal peptide (VIP) and the structurally related pituitary adenylate cyclase-activating polypeptide (PACAP), two neuropeptides present

PLoS-ONE:揭示肠道免疫细胞之间相互作用发生的分子机制

  近日,一项刊登在国际杂志PLoS ONE上题为“Innate CD8αα+ cells promote ILC1-like intraepithelial lymphocyte homeostasis and intestinal inflammation”的研究报告中,来自范德堡大学的科学家们

IL10基因编码功能及结构描述

这个基因编码的蛋白质是一种主要由单核细胞产生的细胞因子,在一定程度上由淋巴细胞产生。这种细胞因子在免疫调节和炎症中有多种作用。下调巨噬细胞th1细胞因子、mhcⅡ类ags和共刺激分子的表达。它还可以增强b细胞的存活、增殖和抗体的产生。该细胞因子可阻断NF-卡帕B活性,参与JAK-STAT信号通路的调

IL10基因编码功能及结构描述

这个基因编码的蛋白质是一种主要由单核细胞产生的细胞因子,在一定程度上由淋巴细胞产生。这种细胞因子在免疫调节和炎症中有多种作用。下调巨噬细胞th1细胞因子、mhcⅡ类ags和共刺激分子的表达。它还可以增强b细胞的存活、增殖和抗体的产生。该细胞因子可阻断NF-卡帕B活性,参与JAK-STAT信号通路的调

在肠道上皮内罗伊氏乳杆菌诱导促进耐受性的T细胞产生

  免疫细胞在肠道中巡逻,确保隐藏在我们吃的食物中的有害细菌不会入侵人体。能够触发炎症的免疫细胞与促进免疫耐受性的免疫细胞处于平衡,从而在不会让敏感组织遭受损伤的情形下保护人体。当这种平衡过于偏向炎症时,炎症性肠病就会产生。  如今,在一项新的研究中,来自美国华盛顿大学医学院和普林斯顿大学的研究人员

The-OP9DL1-System:-Generation-of-TLymphocytes-from-Embryonic4

TROUBLESHOOTINGProblem: The OP9 cells are more than 80%-90% confluent.Solution: It is important when creating working stocks of OP9 cells for freezing

The-OP9DL1-System:-Generation-of-TLymphocytes-from-Embryonic1

The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zúñiga-Pflücker1Sunn

The-OP9DL1-System:-Generation-of-TLymphocytes-from-Embryonic2

Freezing ESCs 25. Passage the ESCs as described in Step 22. Resuspend the cells in 3 mL of freezing medium for OP9 cells. 26. Aliquot 1 mL of cell sus

The-OP9DL1-System:-Generation-of-TLymphocytes-from-Embryonic3

Protocol 3: Fetal Liver-Derived HSC Differentiation on OP9-DL1 CellsDay 0: Initiation of Fetal Liver Co-culture52. Isolate liver tissue from eight to

挑战常规!同一个T细胞受体诱导两种T细胞亚型产生

  每个新形成的T细胞携带一种独特的T细胞受体(T cell receptor, TCR),其中这种TCR识别一种特定的抗原。但是一种给定的TCR如何影响T细胞及其后代的命运仍然在很大程度上是未知的。如今,在一项新的研究中,来自美国麻省理工学院(MIT)的研究人员发现具有完全相同的TCR的T细胞前体

Adhesion-Molecules-on-Lymphocyte

B cell and T cell lymphocytes interact with a variety of cells as part of their immune function, circulating and homing in on specific stimuli in tiss

非典型腺细胞的分类介绍

  根据其细胞病理学形态又可将AGC分为:  (1)非典型腺细胞,不能明确意义(AGC-NOS);  (2)非典型腺细胞,倾向于上皮内瘤变(cervical glandular intraepithelial neoplasm,简称CGIN);  (3)非典型腺细胞,倾向于宫颈管原位腺癌(adeno

巨噬细胞和单核白细胞

·         Lymphocyte Transformation (Donis-Keller lab)Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocytes) from antico

Isolation-of-liver-lymphocyte

Isolation of liver lymphocyte   Several lymphocyte subpopulations reside in the normal adult human liver. Liver lymphocytes mainly include a large n

全球首款人类诱导多能干细胞的小肠上皮细胞产品上市

  Takara Bio正式发售世界上第一个商用的来源于人类诱导多能干细胞(human induced pluripotent stem cells,hiPSCs)的小肠上皮细胞。新发售的人iPSC来源小肠上皮细胞Cellartis Intestinal Epithelial Cells (from

淋巴细胞的组成部分

  淋巴细胞(lymphocyte)血液的有形成分之一。直径约6~18微米,有大、中、小3种。小淋巴细胞约占90%,核呈圆形式卵圆形,核一侧有凹痕,胞质少,嗜碱性。根据免疫学可将淋巴细胞分为T、B两类。T淋巴细胞在胸腺内增生、繁殖,又名胸腺依赖性淋巴细胞(T-lymphocytes)可排斥与识别异物

肠炎造模:DSS在诱导非啮齿类动物肠炎模型的作用3

4. 猪—Swine背景介绍 分别将约克夏猪和西藏猪的肠道菌群移植入健康的新生仔猪中,建立「干预」模型。由 DSS 诱导成急性肠炎,激活了植入约克夏猪肠道菌群仔猪的结肠组织中的 TLR/NLR 信号通路,造成促炎性细胞因子和免疫细胞增加,结肠损伤。而采用西藏猪肠道菌群干预的仔猪并没有显著的炎

与肾癌相关的CDX2基因编码功能描述

该基因是尾相关同源盒转录因子基因家族的成员。编码蛋白是参与细胞生长和分化的肠道特异性基因的主要调节因子。这种蛋白也在肠道的早期胚胎发育中起作用。这种基因的异常表达与肠道炎症和肿瘤发生有关。This gene is a member of the caudal-related homeobox tra

与结直肠癌相关的CDX2基因编码功能描述

该基因是尾相关同源盒转录因子基因家族的成员。编码蛋白是参与细胞生长和分化的肠道特异性基因的主要调节因子。这种蛋白也在肠道的早期胚胎发育中起作用。这种基因的异常表达与肠道炎症和肿瘤发生有关。This gene is a member of the caudal-related homeobox tra

CDX2基因突变与药物因子介绍

该基因是尾相关同源盒转录因子基因家族的成员。编码蛋白是参与细胞生长和分化的肠道特异性基因的主要调节因子。这种蛋白也在肠道的早期胚胎发育中起作用。这种基因的异常表达与肠道炎症和肿瘤发生有关。This gene is a member of the caudal-related homeobox tra

CDX2基因编码功能及结构描述

该基因是尾相关同源盒转录因子基因家族的成员。编码蛋白是参与细胞生长和分化的肠道特异性基因的主要调节因子。这种蛋白也在肠道的早期胚胎发育中起作用。这种基因的异常表达与肠道炎症和肿瘤发生有关。This gene is a member of the caudal-related homeobox tra

高通量测序技术在肠道菌群多样性研究中的应用(三)

一、饮食、能量摄取、肥胖与肠道菌群之间的关系:1.  A core gut microbiome in obese and lean twins——2009《Nature》——if 36.102.  An obesity-associated gut microbiome with increase