Isolationofhumanprostaticepithelialcells
1. A small piece of tissue from each specimen was removed and minced. 2. The tissue was digested with collagenase overnight. 3. To remove the collagenase and the majority of the stromal cells, the tissue was rinsed and centrifuged. 4. The tissue was inoculated into a 60-mm tissue culture dish coated with collagen type I and grown in primary cell system supplemented with growth factors and ho......阅读全文
Human－thyroid－endothelial－cell－isolation
1. Human thyroid glands were obtained at surgery from multinodular goiters.2. The tissue was incubated in 0.1% type II collagenase (Worthington) overn
Stem－cell－characteristics－of－amniotic－epithelial－(AE)－cells
Isolation of AE Cells1. Human placentae were obtained with the approval of the institutional review board, after uncomplicated elective caesarean
Intestinal－intraepithelial－lymphocytes
Intestinal intraepithelial lymphocytes Intestinal intraepithelial lymphocytes (IELs) are mostly T cells, which are continuously associated with gut
Activation－and－Expansion－of－Human－Treg－Cells
实验概要This protocol is intended for activation and expansion of human Treg cells isolated with the Dynal® CD4 CD25 Treg Kit (Cat. no. 113.23D). The exp
Culturing－Human－Neural－Stem－Cells
实验概要Neural stem cells (NSC) are valuable resources because of their ability to differentiate into neurons and glial cells with applications in neur
Purification－of－human－mononuclear－cells－and－neutrophils
PurposeMaterials10ml 6% dextran + 7ml citrate/citric acidDextran: T500 --> 6g+100ml PBSCitrate solution: 25g Na Citrate + 8g citric acid + 500 ml PBS4
Isolation－and－culture－of－porcine－muller－cells
1. Porcine eyes were harvested from pentobarbital-anesthetized animals, each weighing 30 to 40 pounds, and transported to the laboratory in ice-co
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous SystemScott R. Hutton and Larysa H. Pevny1UNC Neuroscience Center
Isolation－and－culture－of－pancreatic－stellate－cells
Pancreatic stellate cells (PaSCs or PSCs) are myofibroblast-like cells found in the areas of the pancreas that have exocrine function. PaSCs are
Isolation－of－osteoblasts－from－human－bone.
Bone samples were cleaned of adherent soft tissue and osteoblasts isolated by two methods.a. The bone was cut into small pieces (2 mm × 2 mm), washed
Isolation－of－human－primary－airway－smooth－muscle
1. Primary cultures of human primary airway smooth muscle (ASM) cells were prepared from explants of airway smooth muscle. 2. The human trachea tiss
Human－Embryonic－Stem－(ES)－Cell－Protocols——Freezing－Human－ES－Cells
Collagenase cells for approximately 7 minutes at 37 °C (until edges of colonies are curling up).With a 5 ml pipet, gently pipet and scrape colonies f
Human－Embryonic－Stem－(ES)－Cell－Protocols——Thawing－Human－ES－cells
Remove Human ES cells from liquid nitrogen storage tank. Fill out a freeze/thaw form.Thaw cryovial by gently swirling in waterbath until only a small
Culture－of－Human－Embryonic－Stem－Cells－(hESC)
All cell lines are initially grown according to the supplier's protocols but we are adapting them to one simple protocol outlined below:6-well pla
Culture－of－human－venous－smooth－muscle－cells
Culture of human venous smooth muscle cells 1. Explants of the 2 groups of skins (control subjects and patients with varicose veins) were prepared
PriCells:－Isolation－of－endothelial－progenitor－cells－(EPCs)
PriCells: Isolation of endothelial progenitor cells (EPCs) 1. Twenty-four mL venous blood was collected at each time point into Vacutainer CPT Mono
Isolation－and－cultivation－of－endothelial－progenitor－cells－(EPCs)
Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue regeneration and substantially contri
Isolation－of－peripheral－blood－mononuclear－cells－(PBMC)
1. Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2. PBMC were isolated by centrifugation over lymphocyte separation medium. 3
Isolation－and－characterization－of－rat－glomerular－endothelial－cells
Glomerular endothelial cells (GECs) from the kidney are in close juxtaposition to other cell types, such as mesangial cells, and may respond to as
Primary－cultures－of－intrahepatic－bile－duct－epithelial－cells－isolated－and...
Primary cultures of intrahepatic bile duct epithelial cells isolated and cultured1. Liver was surgically removed and perfused via the hepatic vein.2.
PriCells:－Questions
Questions 1: Isolation of hepatocytes from other speciesThe basic two-step perfusion procedure can be used for obtaining hepatocytes from various r
Isolation－and－Culture－of－Human－Brain－Tumor－Stem－Cell
The isolation, culture, identification, and purification of stem cells from primary human brain tumors of different phenotypes have marked capacit
Human－Embryonic－Stem－(ES)－Cell－Protocols—Splitting－Human－ES－cells－onMatrige
based on splitting onto on plateWarm collagenase media to 37°C in a water bath.Aspirate media off of cell culture plate.Add the following amount of co
Human－Embryonic－Stem－(ES)－Cell－Protocols——Splitting－Human－ES－cells－on－MEFs
based on splitting onto one plateWarm collagenase IV split media to 37 °C in a water bath.Aspirate media off of cell culture plate.Add the following a
Mesenchymal－progenitor－cells－derived－from－human－muscle
Harvesting traumatized muscle derived mesenchymal progenitor cells (MPCs)1. The muscle tissue was dissected without contaminating by granulation, adip
Noninvasive－Human－Nuclear－Transfer－with－Embryonic－Stem－Cells
Noninvasive Human Nuclear Transfer with Embryonic Stem CellsSohyun L. McElroy1 and Renee A. Reijo PeraCenter for Human Embryonic Stem Cell Research an
Isolation－and－culture－of－rat－coronary－microvascular－endothelial－cells
CMVE were isolated from Wistar rat hearts by digestion of primary cell isolation kit.1. Hearts mounted on a Langendorff apparatus were perfused at 3
ELECTROPORATION－OF－ES－CELLS－AND－ISOLATION－OF－H/R－CLONES
Need 1.5-2 x 107 cells from a 2 day culture.1. Cells are harvested as normal, washed x 1 in PBS then taken up at conc. of 1.2 x 10 7 cells/ml in cold
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells2
Slide Coating13. Just before use, dilute the poly-D-lysine stock solution 1:50 in H2O. Add 0.2 mL of diluted poly-D-lysine to each well of an eight-we
Isolation－of－DL(Low－Density－Lipoprotein)－From－Human－Plasma
This procedure involves use of human plasma, a potentially dangerous source of blood-borne disease. Wear long-cuffed gloves, and eye-protection.Day on