聚丙烯酰胺凝胶电泳(polyacrylamidegelelectrophoresis,PAGE)

配制 Tris- 甘氨酸 SDS-PAGE 聚丙烯酰胺凝胶电泳分离胶所用溶液 溶液成分 不同体积( ml )凝胶液中各成分所需体积( ml ) 5 10 15 20 25 30 40 50 6% SDS-PAGE 水 2.6 5.3 7.9 10.6 13.2 15.9 21.2 26.5 30% 丙烯酰胺溶液 1 2 3 4 5 6 8 10 10% SDS 0.05 0.1 0.15 0.2 0.25 0.3 0.4 ......阅读全文

聚丙烯酰胺凝胶电泳(polyacrylamide-gel-electrophoresis,PAGE)

配制 Tris- 甘氨酸 SDS-PAGE 聚丙烯酰胺凝胶电泳分离胶所用溶液   溶液成分 不同体积( ml )凝胶液中各成分所需体积( ml ) 5 10 15 20 25 30 4

Polyacrylamide-Gel-Electrophoresis-of-Oligonucleotides

1. Pour and polymerize a 20% polyacrylamide gel, no Urea.2. Remove clamps. Rinse with water. Remove comb. Rinse top of gel well.3. Insert comb teeth d

2D-Polyacrylamide-Gel-Electrophoresis

This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor

蛋白质聚丙烯酰胺凝胶电泳(PAGE,polyacrylamide-gel-...2

(3)电荷效应在分离胶中,各种血清蛋白所带静电荷不同,而有不同的迁移率。表面电荷多,则迁移快,反之则慢。因此各种蛋白质按照电荷多少、分子量大小及分子形状以一定顺序排成一个个区带。不连续PAGE所具有的分子筛效应、浓缩效应和电荷效应大大提高了它的分辨率。电泳后蛋白质染色目前常用的是考马斯亮蓝法,其比氨

蛋白质聚丙烯酰胺凝胶电泳(PAGE,polyacrylamide-gel-...3

【目的和要求】1. 掌握聚丙烯酰胺凝胶电泳的基本原理2. 掌握垂直板状凝胶电泳槽的使用和凝胶的配制方法。3. 学会利用相对迁移率分析蛋白质种类。【实验原理】带电颗粒在电场的作用下,向着与其相反的电极移动,称为电泳。电泳做为一种物质分离及鉴定技术,其原理在于任一物质质点,由于其本身的解离作用或由于表面

RNA的聚丙烯酰胺凝胶电泳(PAGE;-polyacrylamide-gel-electrophor...

一、原理核酸(ribonucleicacid,RNA)分子在一定pH值的缓冲液中带有电荷,将其放入电场中,可向与其所带电荷电性相反的电极移动。聚丙烯酰胺凝胶具有分子筛效应,核酸分子大小、形状不同,故在电场作用下,核酸分子在聚丙烯酰胺凝胶中泳动速度不同,依此可达到分离纯化的目的。 二、材料、仪器设

血清脂蛋白聚丙烯酰胺凝胶电泳(PAGE;polyacrylamide-gel-electr

一 原理:  聚丙烯酰胺凝胶电泳,具有分子筛和电泳的双重作用,它的分辨力高,以此为电泳载体,可以将血清脂蛋白各组分分离。  血清中脂类物质均与血清载脂蛋白结合成水溶性的蛋白形式存在。各种脂蛋白中所含载脂蛋白的种类和数量不同,各种脂蛋白颗粒大小也相差较大,因此,用聚丙烯酰胺电泳,血清中的脂蛋白,可根据

蛋白质电泳

蛋白质电泳(主要内容如下)One-Dimensional SDS-PAGETwo-Demensional SDS-PAGEProtein Electrophoresis in Agarose Gel Gel StainingRecipesOne-Dimensional SDS-PAGE·      

ELECTROPHORESIS-OF-DNA-IN-POLYACRYLAMIDE-GELS

ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELSGel SizesSmall:             165 x 130 mmMedium:         165 x 200 mmLarge:            165 x 260 mm5% Anal

聚丙烯酰胺凝胶电泳的定义

聚丙烯酰胺凝胶电泳( polyacrylamide gel electrophoresis,简称PAGE),是以聚丙烯酰胺凝胶作为支持介质的一种常用电泳技术,用于分离蛋白质和寡核苷酸。

聚丙烯酰胺凝胶电泳

聚丙烯酰胺凝胶电泳( polyacrylamide gel electrophoresis,简称PAGE),是以聚丙烯酰胺凝胶作为支持介质的一种常用电泳技术,用于分离蛋白质和寡核苷酸。

什么是聚丙烯酰胺凝胶电泳?

聚丙烯酰胺凝胶电泳( polyacrylamide gel electrophoresis,简称PAGE),是以聚丙烯酰胺凝胶作为支持介质的一种常用电泳技术,用于分离蛋白质和寡核苷酸。

聚丙烯酰胺凝胶电泳的定义

聚丙烯酰胺凝胶电泳( polyacrylamide gel electrophoresis,简称PAGE),是以聚丙烯酰胺凝胶作为支持介质的一种常用电泳技术,用于分离蛋白质和寡核苷酸。

等电点聚焦电泳的技术介绍

聚丙烯酰胺凝胶电泳( polyacrylamide gel electrophoresis,简称PAGE),是以聚丙烯酰胺凝胶作为支持介质的一种常用电泳技术,用于分离蛋白质和寡核苷酸。

RNA电泳

RNA Gel (Crawford Lab)Gel Electrophoresis of RNA (Beverly Faulkner-Jones)great tips on RNA gel electrophoresis.Northern Gel and  TransferUsing glyoxal

Gel-Electrophoresis-of-DNA

What is Electrophoresis?Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab we

RNA-gel-electrophoresis

实验概要RNA gel electrophoresis主要试剂DEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide sol

Agarose-Gel-Electrophoresis

实验概要Separating nucleic acid fragments by agarose gel electrophoresis.实验原理 Agarose  gel electrophoresis remains the most widely used technique for  sep

Agarose-gel-electrophoresis

General ProcedureCast a gelPlace it in gel box in running bufferLoad samplesRun the gelImage the gelCasting Gels0.7% agarose gel with 1kbp ladder in U

RNA-gel-electrophoresis

MaterialsDEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide solutions should also be

RNA电泳

·         RNA Gel (Crawford Lab)·         Gel Electrophoresis of RNA (Beverly Faulkner-Jones)great tips on RNA gel electrophoresis. ·         Northern

聚丙烯酰胺凝胶电泳简介

  聚丙烯酰胺凝胶电泳(英语: polyacrylamide gel electrophoresis,简称PAGE) ,是以聚丙烯酰胺凝胶作为支持介质的一种常用电泳技术,用于分离蛋白质和寡核苷酸。  作用原理:聚丙烯酰胺凝胶为网状结构,具有分子筛效应。它有两种形式:非变性聚丙烯酰胺凝胶电泳(Nati

Agarose-Gel-Electrophoresis-of-DNA

1) Dissolve 1 g of agarose in 100 ml of 1X TAE or TBE buffer (gives a 1% gel). See note for making LMP agarose gel. 2) Cast the gel with the comb in p

Alkaline-agarose-gel-electrophoresis

Alkaline agarose gel electrophoresis (Sambrook et al., 1989)Alkaline agarose gels can be used to determine the size and quality of first and second st

Blue-Native-Gel-Electrophoresis

Blue Native Gel ElectrophoresisStock solutions49.5%T, 3%C Acrylamide 24 g acrylamide, 0.75 g bisacrylamide / 50 ml H2O Store at RT3 x Gel buffer 150 m

InGel-Digestion-of-Proteins-Separated-byPolyacrylamide-Gel-Electrophoresis

1. Excision of protein bands (spots) from polyacrylamide gelsRinse the gloves you use with water to avoid traces of dust in your sample.Rinse the gel

SDS-Gel-Electrophoresis-of-Tubulin\MAPs

MaterialsStock Acrylamide: (30%T:0.8%C)30% by weight of acrylamide0.8% by weight of N,N'-bis-methylene acrylamideSeparation Gel (Final Concentrati

High-Resolution-Agarose-Gel-Electrophoresis

实验概要Agarose gel  electrophoresis remains the most widely used technique for separating  nucleic acid fragments due to its ease of use, non-toxicity, a

Denaturing-Agarose-Gel-Electrophoresis-of-RNA

The overall quality of an RNA preparation may be assessed by electrophoresis on a denaturing agarose gel; this will also give some information about R

Denaturing-Gradient-Gel-Electrophoresis-(DGGE)

Purpose:Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to h