AtypicalWesternt...

实验概要Peprotech provides a typical Western-transfer protocol (e.g. for the detection of protein X).实验原理Western Transfer, also known as Western Blotting, is a rapid immunoblotting technique for identifying the presence of a particular protein in a complex mixture of proteins such as cell lysates or sera. The technique exploits both the efficiency of SDS-PAGE to separate a mixture of proteins into distinct prot......阅读全文

A-typical-Westernt...

实验概要Peprotech provides a typical Western-transfer protocol (e.g. for the detection of protein X).实验原理Western Transfer, also known as Western Blottin

Typical-Western-Tra...

实验概要Peprotech provides a typical western transfer and development protocol.实验原理Western Transfer, also known as Western Blotting, is a rapid immunobl

气相离子迁移谱的技术指标有哪些

  气相离子迁移谱仪(GC-IMS)是一款多气体分析仪,其中离子迁移谱仪(IMS),能在较低的ppb范围内具有较高的灵敏度和高分辨率,配备气相色谱柱(GC)后能进一步提高选择性并降低交叉敏感度,使用这种微量气体分析仪,可以检测和识别有毒气体,而不需要在非常低的浓度水平下直接就地进行富集。  气相色谱

Basics-of-Electrochemical-Impedance-Spectroscopy(六)

Purely Capacitive CoatingA metal covered with an undamaged coating generally has a very high impedance. The equivalent circuit for such a situatio

SCI论文中怎样描述TEM结果?样品有怎样的形貌

鉴于有同学问我对于SCI论文写作这个部分的更新计划,我在这里稍微跟大家说一下。这个部分肯定不仅仅是列出一些常规实验结果如何描述这么简单。现在发的这些呢,只是一些基础准备。在将这些准备工作做完了之后,我们会按照论文的结构,对每个部分进行详细讨论,尽可能把我们这些年学到的有用的东西分享给大家,给大家提供

Culture-of-human-renal-proximal-tubule-cells-and-renal-cortical-fibroblasts

1. The method for primary culture of human renal proximal tubule cells (PTC) and renal cortical fibroblasts (CF) is described in detail.2. Renal cor

Detection-of-Mycoplasma-by-Culture

AimDetection of mycoplasma by culture is the reference method of detection and has a theoretical level of detection of 1 colony-forming unit (cfu). Ho

NEOFOX氧传感系统

NeoFox是一款体积小的相位荧光计,可同海洋光学的氧传感器探头及感应片相接,不论气相还是液相都能提供快速可靠的氧测量。 产品详情                               体积小简单的USB接口模拟输出RS-232支持非常适合以下领域的测量:工业生产过程医疗和医药生物/细胞培养/

Detection-and-Measurement-of-Radioactivity

Liquid scintillation countingThe amount of kinetic energy in a beta particle differs from one decay to the next. However, each radioisotope has a typi

Enzyme-Kinetics-assay-of-the-WT

To assay 17 b-HSD activity in lysates, cells were harvested 48h after transfection using PBS Enzyme Free Cell Dissociation Solution ( specialty Media

Comparison-of-lysis-methods

Comparison of lysis methodsSonicationmost efficient method of cell fractionationproblem: heat build up which can denature proteins (proportionate to l

Size-and-Shape-of-Protein-Molecules2

The sedimentation coefficient of a protein is a measure of how fast it moves through the gradient. Increasing the mass of the protein will increase it

基于epMotion-5075t系统与KPPA-HyperPlus试剂盒的全自动测序...2

Results and DiscussionThe post-ligation qPCR results were used to calculate the percentage of starting material that was successfully adapter ligate

原装进口树脂-制备纯水用-混床阴树脂技术参数介绍

  产品详情:   Tulsimer ®A-23均粒强碱型阴离子交换树脂,适用于超纯水系统的混床用离子交换树脂   Tulsimer ®A-23强碱型离子交换树脂 ,是一款苯乙稀系列具有四级氨的高品质Type-1阴性离子交换树脂。并同时拥有绝佳的物理及化学穏定品貭。可应用于汽电共生发电厂冷凝水处

Genetic-Engineering-of-Novel-Flower-Colors-in-Floricultural-Plants:-Recen..

Since the first successful genetic engineering of flower color in petunia, several new techniques have been developed and applied to modify flower

SMARCC1基因编码的功能和结构描述

这个基因编码的蛋白质是SWI/SNF蛋白家族的一员,其成员具有螺旋酶和ATPase活性,被认为通过改变这些基因周围的染色质结构来调节某些基因的转录编码蛋白是大的ATP依赖性染色质重塑复合物SNF/SWI的一部分,包含一个预测的亮氨酸拉链基序,这是许多转录因子的典型特征。The protein enc

Bradford-Assay

The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue

Bradford-Assay

Bradford AssayThe bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie B

纯进口品牌制备纯水用-混床阳树脂使用说明

  产品详情:   Tulsimer ®T-42特级凝胶型强酸性阳离子交换树脂,适用于超纯水系统的混床用阳离子交换树脂   Tulsimer ®T-42是特级强酸型离子交换树脂,氢 H+/钠 Na+阳离子交换树脂, 是一款有较高的交换容量 ,并同时拥有绝佳的物理及化学稳定品质。可应用于汽电共生发

SMARCC1基因突变因子与药物介绍

这个基因编码的蛋白质是SWI/SNF蛋白家族的一员,其成员具有螺旋酶和ATPase活性,被认为通过改变这些基因周围的染色质结构来调节某些基因的转录编码蛋白是大的ATP依赖性染色质重塑复合物SNF/SWI的一部分,包含一个预测的亮氨酸拉链基序,这是许多转录因子的典型特征[由RefSeq提供,2008年

SMARCC2基因编码的功能和结构描述

这个基因编码的蛋白质是SWI/SNF蛋白家族的一员,其成员具有螺旋酶和ATPase活性,被认为通过改变这些基因周围的染色质结构来调节某些基因的转录编码蛋白是大的ATP依赖性染色质重塑复合物SNF/SWI的一部分,包含一个预测的亮氨酸拉链基序,这是许多转录因子的典型特征另外,已经发现该基因编码不同亚型

Rapid-Isolation-and-Purification-of-Photosystem-I-ChlorophyllBinding...

The available procedures for isolation and purification of photosystem I (PSI) from Chlamydomonas reinhardtii are time consuming and usually require

How-to-Make-Simple-Solutions-and-Dilutions

1. Simple Dilution (Dilution Factor Method based on ratios)A simple dilution is one in which a unit volume of a liquid material of interest is combine

SMARCC2基因突变因子与药物介绍

这个基因编码的蛋白质是SWI/SNF蛋白家族的一员,其成员具有螺旋酶和ATPase活性,被认为通过改变这些基因周围的染色质结构来调节某些基因的转录编码蛋白是大的ATP依赖性染色质重塑复合物SNF/SWI的一部分,包含一个预测的亮氨酸拉链基序,这是许多转录因子的典型特征另外,已经发现该基因编码不同亚型

RPS7基因编码的功能和结构描述

核糖体是催化蛋白质合成的细胞器,由一个小的40s亚基和一个大的60s亚基组成。这些亚基由4种RNA和大约80种结构上不同的蛋白质组成。这个基因编码一个核糖体蛋白,它是40S亚单位的一个组成部分该蛋白属于核糖体蛋白s7e家族。它位于细胞质中。作为编码核糖体蛋白的典型基因,有多个经过处理的假基因散布在基

Microbiology--Serial-Dilution

1. An undiluted (stock) solution is termed the 100 dilution. This may be a chemical solution, or a suspension of bacteria or bacteriophage.2. Typical

石墨烯在太赫兹频段实现的无线片上网络(WiNoC)(二)

2. System Model of Nanocommunications in a GWiNoCFigure 1 illustrates a typical GWiNoC package where two on-chip cores  and  are both equipped with

质粒的小量制备

·         Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and recipe for solution I, II and III.Alkaline Lysis Minip

质粒的小量制备

·         Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and recipe for solution I, II and III.Alkaline Lysis Minip

Cell-and-tissue-lysis-hub

This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison