MicrobiologySerialDilution
1. An undiluted (stock) solution is termed the 100 dilution. This may be a chemical solution, or a suspension of bacteria or bacteriophage.2. Typical diluions are 10-fold dilutions; each dilution is 1/10th the concentration of the previous dilution. The first 10-fold dilution is termed the 10-1 dilution.3. Each subsequent dilution is made from a previous dilution. Here is a series of 10-fold dilutions: Star......阅读全文
Microbiology－－Serial－Dilution
1. An undiluted (stock) solution is termed the 100 dilution. This may be a chemical solution, or a suspension of bacteria or bacteriophage.2. Typical
Cloning－by－Limiting－Dilution－of－Hybridoma
Author: Nanci DonackiSource: Contributed by Nanci DonackiDate Added: Tue May 14 2002Date Modified: Tue Apr 27 2004MaterialsDMEM, high glucose (Life Te
How－to－Make－Simple－Solutions－and－Dilutions
1. Simple Dilution (Dilution Factor Method based on ratios)A simple dilution is one in which a unit volume of a liquid material of interest is combine
Streptomyces:Protocols/Conjugation
Intergeneric Conjugation and OverlayDescription Transfer of plasmid/cosmid DNA from a host strain, e.g. E.coli ET12567 [pUZ8002], to the recipient str
Agglutination－Assays
Agglutination AssaysREFERENCE: Lanyi, B., and T. Bergan. Methods in Microbiology, Vol 10: 93-168. BACTERIAL AGGLUTINATION: Bacterial agglutination is
基于epMotion－5075t及KAPA文库定量试剂盒的全自动NGS文库...2
Results and DiscussionAutomated qPCR Setup Accuracy and PrecisionThe accuracy and precision of the qPCR setup was evalu ated based on standard curve
Serial－Femtosecond－Crystallography的基本原理是什么？
X-ray Diffraction。区别于同步辐射X-ray Crystallography的最大之处在于光源特点的不同。
Virus－Infection－of－Fibroblasts
Procedure: Day One 1. Plate cells in 60 mm dishes using 5 ml DMEM containing 10% Calf Serum. The protocol has worked well for NIH3T3 with 1 x 105 cell
Virus－Infection－of－Fibroblasts
Procedure: Day One 1. Plate cells in 60 mm dishes using 5 ml DMEM containing 10% Calf Serum. The protocol has worked well for NIH3T3 with 1 x 105 cell
Phage－Titer
IntroductionLambda phage is a commonly used vector for transgenes. Its very high rate of infectivity makes it ideal for creating large numbers of clon
酶－联－免－疫－吸－附－实－验2
十 字 交 叉 连 续 稀 释 分 析 法 （criss-cross serial-dilution analysis) 测定试剂最适浓度实验步骤展开
Trends－in－Microbiology：那些被吃进去的益生菌
肠道微生物群是存在人体肠道的微生物群体的总称，其中包括了细菌、真菌、古菌以及原生物动植物（甚至病毒）等种类众多的微生物。由于测序技术的提高，近二十年来，科学界越来越关注肠道微生物菌群。也有很多的证据表明，肠道微生物菌群对于人体的健康有着很大影响。这种影响不仅局限于肠道，还可能对大脑、肝脏这样的器
Trends－in－Microbiology：那些被吃进去的益生菌
肠道微生物群是存在人体肠道的微生物群体的总称，其中包括了细菌、真菌、古菌以及原生物动植物（甚至病毒）等种类众多的微生物。由于测序技术的提高，近二十年来，科学界越来越关注肠道微生物菌群。 也有很多的证据表明，肠道微生物菌群对于人体的健康有着很大影响。这种影响不仅局限于肠道，还可能对大脑、肝脏这样
Neutralizing－Bioassay－Protocols
Neutralizing Bioassay ProtocolsIntroductionAntibodies that block binding of cytokines to their specific receptors and neutralize their effects are cri
Environmental－Microbiology：天然抗癌药物的真正起源
几十年来,科学家已经知道ET -743是一种从海洋无脊椎动物中提取的称为被囊红树蝰的药物，该药物可以杀死癌细胞。在欧洲和美国临床试验中该药物已经批准用于病人。科学家们怀疑红树蝰被囊动物是一种海鞘类生物，实际上并不能生产ET -743。但该药（曲贝替丁）的确切起源仍是一个谜。 通过使用先进的
《Nature－Microbiology》发表合成生物学研究新工具
过去17年里，科学家和工程师们开发了可编程的功能性活细胞合成基因电路。类似无数电子产品的集成电路，人工基因电路也能生成自定义动态、再接内生网络、感知环境刺激、生产有价值的生物分子。 合成生物学在医学和生物技术领域的应用前景非常广泛，例如狙击超级病菌、生产高级生物原料、制造先进功能性材料等等。
Microbiology：发现可将植物废料直接变成柴油的真菌
一种能将植物废料直接变成柴油的真菌可以让我们不消耗粮食的情况下生产出生物燃料。这种真菌就是美国科学家在巴塔哥尼亚的雨林树木中发现的，叫粉红粘帚霉。它可以让柴油变成气体，较液体燃料更加容易提炼、纯化和贮存。 发现此真菌的美国蒙大纳州大学保兹曼分校的加里·斯托贝尔说：“地球上没有其它已知生物能做到这个
Nature－Microbiology：为什么某些EB病毒会引起癌症？
EB病毒(EBV)非常广泛。世界上90%以上的人口受到感染，后果各不相同。虽然这种感染通常不会感染人类，但在某些情况下，它会导致腺热或各种癌症。德国癌症研究中心(DKFZ)的研究人员现在已经发现了为什么不同的病毒株会导致非常不同的病程。 超过90%的人在一生中感染了EB病毒，但感染通常不会被发
Competitive－ELISA
DAY 11. Coat Nunc immuno-module plates overnight, in usual manner.2. Set up competition assay between antibody and competing substance :-Prepare a 1:2
Ligation－Optimization
The following protocol can be used to optimize ligation conditions for difficult to clone (e.g. very large) fragments. The principle is to independent
SemiQuantitative－Measurement－of－Proteins－by－Dot－Blotting
Purpose...Concentration of proteins in a crude preparations (such as culture sup) can be estimated semi-quantitatively by using "Dot Blot" method if y
Preparation－of－Phage－Lysates
Preparation of Phage LysatesInoculate 5 ml of lambda-broth in a glass culture tube with a single colony of an appropriate host strain of E. coli. Incu
Transformation－of－Electrocompetent－E.－coli－with－Blue/White－selection
Desalt DNA template by EtOH precipitation in NaOAc followed by at least 2x washes with 70% EtOH. Resuspend in 5 - 15 µL of sterile H2O.Rinse cuvettes
医学微生物学(Medical－Microbiology)词汇归纳（三）
domain 域,结构域,功能区donor site 给位double helix 双螺旋effector 效应器,效应物elongation 延长endopeptidase 内肽酶enhancer 增强子enolphosphopyruvate 磷酸烯醇式丙酮酸 enzyme 酶es
医学微生物学(Medical－Microbiology)词汇归纳（二）
pyrogen 致热源rabies virus 狂犬病病毒recombination 重组合，基因重组release 释放retrovirus 逆转录病毒reverse transcriptase 逆转录酶Salmonella typhi 伤寒沙门菌scarlet fever 猩红热septicem
Nature Microbiology:解析调控肠道免疫炎症平衡的新机制
由中国科学院生物物理研究所感染与免疫重点实验室果蝇研究团队完成的论文Bap180/Baf180 is required to maintain homeostasis of intestinal innate immune response in Drosophila and mice 以art
医学微生物学(Medical－Microbiology)词汇归纳（一）
adenovirus 腺病毒 adsorption 吸附aerobe 需氧菌aflatoxin 黄曲霉毒素airborne transmission 空气传播amantadine 金刚烷胺anaerobe 厌氧菌anthrax 炭疽antigenic drift 抗原漂移,抗原转变asepsis 无
Cytokine－Bioassays
Cytokine BioassaysIntroductionBiological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary cel
实验室自动化与筛选协会2013亚洲会展新品发布
2 kinds of newly launched products are available to SLAS Exhibitors to distribute on 2013 SLAS Asia Conference & Exhibition website. This is
Nature－Reviews－Microbiology：撰写极端环境微生物多样性综述
地球上广泛分布着包括地热泉、深海热溢口、冰冻圈、高盐生境、深地以及矿山酸性废水等各种极端环境（图1）。尽管理化条件严酷，这些生境给来自所有三个生命域的众多微生物提供多样化的生态位。这些独特的微生物进化出了不同的策略以应对多种极端环境胁迫，定义地球生命的物理和化学极限，并吸引了从基础理论到生物技术