immunofluorescenceofgeneralPBMCbyPeprotech
实验概要The following protocol provides a method of immunofluorescence of general PBMC by Peprotech.实验步骤The following protocol used human PBMC that were isolated from whole human blood(Ficoll method) and plated onto fibronectin-coated coverslips in 10% FCS/DMEMsupplemented with pen/strep and L-glutamine. Cells were allowed to adhere at 37˚Cfor 1 hour before being fixed in 4% paraformaldehyde for 20 minutes at r......阅读全文
immunofluorescence of general PBMC by Peprotech
实验概要The following protocol provides a method of immunofluorescence of general PBMC by Peprotech.实验步骤The following protocol used human PBMC that were
immunofluorescence of general cell by Peprotech
实验概要The following protocol provides a method of immunofluorescence of general cell by Peprotech.实验步骤Please refer to the antibody Product Information S
immunofluorescence of rabbit anti-murine RELMβ by Peprotech
实验概要 The following protocol provides a method of immunofluorescence of rabbit anti-murine RELMβ by Peprotech. 实验步骤 The following protocol used B
immunofluorescence of rabbit anti-murine leptin by Peprotech
实验概要 The following protocol provides a method of immunofluorescence of rabbit anti-murine leptin by Peprotech. 实验步骤 The following protocol used
immunofluorescence of rabbit anti-murine RELMα by Peprotech
实验概要The following protocol provides a method of immunofluorescence of rabbit anti-murine RELMα by Peprotech.实验步骤The following protocol used B6 mice
immunofluorescence of rabbit anti-murine VEGF by Peprotech
实验概要The following protocol provides a method of immunofluorescence of rabbit anti-murine VEGF by Peprotech.实验步骤The following protocol used normal muri
immunofluorescence of mouse anti-human IL-6 by Peprotech
实验概要The following protocol provides a method of immunofluorescence of mouse anti-human IL-6 by Peprotech.实验步骤The following protocol used human endomet
General Reference
Units1 mg = 10-3 g1 ug = 10-6 g1 ng = 10-9 g1 pg = 10-12 g1 kb of double stranded DNA = 660 kD 1 kb of single stranded DNA = 330 kD 1 kb of single str
Immunofluorescence ...
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
General Cloning Protocols
Large Scale Preps: (See Large scale plsasmid prep protocol for more details) Cultures: Inoculate a 5 mL LB/Amp (50 - 100 µg/mL) culture in early a