GeneralLaboratoryMethodsforTetrapyrroles

There are thousands of porphyrins and metalloporphyrins, and hundreds of new derivatives appear each year. This variety arises because the cyclic conjugated tetrapyrrole nucleus (Figure 1 ) can have different substituents at the eight β-pyrrole positions, at the four meso [5,10,15,20] carbon atoms, and N-alkyl groups can be added to the four central nitrogen atoms. Since its synthesis in 1972, over 8000......阅读全文

General-Laboratory-Methods-for-Tetrapyrroles

There are thousands of porphyrins and metalloporphyrins, and hundreds of new derivatives appear each year. This variety arises because the cyclic

General-Laboratory-Procedures,-Equipment-Use,-and-Safety-Considerations

A. Storage .The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals promo

Apoptosis:-A-Laboratory-Manual-of-Experimental-Methods-Andrea-Cossarizza

THE CELL 1. Morphological aspects of apoptosis Walter Malorni, Stefano Fais & Carla Fiorentini 2. Cell cycle Miriam Capri & Daniela BarbieriTHE NUCLEU

General-Reference

Units1 mg = 10-3 g1 ug = 10-6 g1 ng = 10-9 g1 pg = 10-12 g1 kb of double stranded DNA = 660 kD 1 kb of single stranded DNA = 330 kD 1 kb of single str

General-Cloning-Protocols

Large Scale Preps: (See Large scale plsasmid prep protocol for more details)Cultures: Inoculate a 5 mL LB/Amp (50 - 100 µg/mL) culture in early a.m. w

TDMSQS-2020-人物篇专家介绍

Prof. Christa M. COBBAERTProfessor of Clinical Chemistry and Laboratory Medicine.Department of Clinical Chemistry and Laboratory Medicine. Leiden Univ

DNA-EXTRACTION-PROCEDURE--GENERAL

Grow cells overnight in 500 ml broth medium.Pellet cells by centrifugation, and resuspend in 5 ml 50 mM Tris (pH 8.0), 50 mM EDTA.Freeze cell suspensi

immunofluorescence-of-general-cell-by-Peprotech

实验概要The following protocol provides a method of immunofluorescence of general cell by Peprotech.实验步骤Please refer to the antibody Product Information S

immunofluorescence-of-general-PBMC-by-Peprotech

实验概要The following protocol provides a method of immunofluorescence of general PBMC by Peprotech.实验步骤The following protocol used human PBMC that were

ELISA-Protocol-(General-Guidelines)

实验概要Sandwich  enzyme-linked immunosorbent assays (ELISAs) involve attachment of a  capture antibody to a solid phase support. Samples containing known

ELISA-Protocol-(General-Guidelines)

实验概要Sandwich  enzyme-linked immunosorbent assays (ELISAs) involve attachment of a  capture antibody to a solid phase support. Samples containing known

Guidelines-for-the-Use-of-Analgesics-and-Tranquilizers-in-Laboratory-Animal

What is Anesthesia? Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pain relief), amn

RNase-and-DEPC-Treatment:-Fact-or-Laboratory-Myth

Researchers are usually trained in RNA isolation and analysis methods by one another or by technical manuals. Experimental procedures are often not qu

BJUT-and-H.E.L.-Joint-LaboratoryCollaborative-Excellence-for-a-Bright-Future

  By the end of 2023, the number of new energy vehicles in China reached 20.4 million; whether it's power batteries or new energy vehicles, China

集锦-|-NG-General系列氮气发生器

  今年4月,普敦科技将氮气发生器产品线进行了全面的升级,新款NG General系列已正式上线。  目前,普敦氮气发生器拥有【膜分离技术】和【分子筛技术】的一体式/分体式氮气发生器,可以供各品牌的LC-MS、氮吹仪、ELSD/CAD、液氮发生器等设备用气。  一体式 氮气发生器  01【膜分离】 

Comparison-of-lysis-methods

Comparison of lysis methodsSonicationmost efficient method of cell fractionationproblem: heat build up which can denature proteins (proportionate to l

Staining-Methods-for-cell

death Z. Xia 10/2/95The simplest way: trypan blue. Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells; P.I. (propidiu

ICBR-Flow-Cytometry-Core-Laboratory-Paraformaldehyde-Fixation-of-Cells

BackgroundThis fixation method is good for cells labelled by fluorochrome-conjugated antibodies to membrane antigens. It will stabilize the light scat

Guidelines-for-theUse-of-Analgesics-and-Tranquilizers-in-Laboratory-Animal4

Other AnalgesicsAnalgesics are pain relievers most often given after a surgery. Narcotic analgesics have already been described above. Nonsteroidal an

Guidelines-for-theUse-of-Analgesics-and-Tranquilizers-in-Laboratory-Animal3

BarbituratesThe barbiturates are an acid ring molecule with various ring substitutes that imbue the drug with different properties. Barbiturates are a

Laboratory-Investigation:新药可预防致命核辐射

  新研究发现对抗致命的辐射有了新突破。在核辐射暴露后24小时内,一种再生肽可以帮助显著提高小鼠的生存时间。目前这项研究出版在自然出版集团的杂志《Laboratory Investigation》上。  德州大学医学分部研究作者Carla Kantara是生物化学和分子生物学博士后,他说当小鼠处于致

Guidelines-for-theUse-of-Analgesics-and-Tranquilizers-in-Laboratory-Animal7

ThermoregulationAnimals frequently become hypothermic during anesthesia because of inhalation of cold gases, exposure of body cavities to the room air

Guidelines-for-theUse-of-Analgesics-and-Tranquilizers-in-Laboratory-Animal5

Anesthetic Induction and MaintenanceInjectable AnesthesiaAnesthetic induction using injectable anesthetics is fairly simple. It involves admininsistra

Guidelines-for-Retroorbital-Bleeding-in-Laboratory-Rats-and-Mice

Materials Needed:Micro pipettes (l00 µl) or Pasteur pipettes drawn to fine tip2X2 gauze squaresNon-sterile glovesEppendorf or other tubes to hold samp

Guidelines-for-theUse-of-Analgesics-and-Tranquilizers-in-Laboratory-Animal2

GeneralSome of the drugs listed here do not possess all three criteria for an anesthetic and must be used in combinations to achieve full anesthesia o

Guidelines-for-theUse-of-Analgesics-and-Tranquilizers-in-Laboratory-Animal6

 Anesthetic MachineThe best method of delivering an inhalant anesthetic is with an anesthetic machine. These machines precisely mix the gas with air o

重组DNA的分离、克隆与测序实验手册

 edited by                Bruce A. Roe                Judy S. Crabtreeand Akbar S. KhanDepartment of Chemistry and Biochemistry                The Uni

美国工程院公布2017年入选院士名单

   美国当地时间2月8日,美国工程院公布2017年入选院士名单,共新增84位院士及22位外籍院士。其中有多位著名华人学者,包括黄永刚、沈向洋、张东晓、丛京生、陈向力等人。  美国国家工程院院士是工程界最高荣誉之一,授予那些在工程领域内从事研究、实践和教育并作出卓越贡献的人士;以及在新兴和发展中的技

Analysis-and-Reconstitution-of-Phycobiliproteins:-Methods-for-the-...

Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment ReactionsPhycobiliproteins are a homologous fam

Plant-Genome-Annotation-Methods

Annotation of plant genomic sequences can be separated into structural and functional annotation. Structural annotation is the foundation of all g