AntigenRetrieval
实验概要Formalin fixed paraffin embedded tissue is a very common preparation for a wide variety of tissue types used in immunohistochemistry. Unfortunately, this process can result in the cross-linking of proteins which can mask binding sites on the antigen of interest. In order to unmask, or retrieve, these cross-linked sites, scientists can utilize a variety of procedures known collectively as antigen retri......阅读全文
Antigen－Retrieval
实验概要Formalin fixed paraffin embedded tissue is a very common preparation for a wide variety of tissue types used in immunohistochemistry. Unfortun
Antigen－Retrieval
实验概要Formalin fixed paraffin embedded tissue is a very common preparation for a wide variety of tissue types used in immunohistochemistry. Unfortunat
Antigen－Processing－and－Presentation
Invariant chain (Ii) associates with newly synthesized class II molecules in the endoplasmic reticulum (ER), an interaction that has been shown to int
什么是抗原(Antigen)－？
凡能刺激机体产生抗体，并能与抗体发生特异性结合的物质称为抗原。物质所具有的这种特性称为抗原性。抗原的物质可以是机体自身的成分如蛋白、酶、多肽或者多糖。也有外来的抗原（如病毒、污染物）。 抗原的特异性：各种抗原物质的化学组成虽然很复杂，但能刺激机体产生抗体并与抗体反应相结合的化学组成，仅仅是抗原物质表
Antigen－Affinity－Purification－of－Antibodies
实验概要To acquire purified antibodies (This method typically yields >95% pure specific antibodies ).实验原理 Cytokines are signaling proteins necessary for
ImmunohistochemistyEnzymatic－Protocol
OverviewR&D Systems provides monoclonal, polyclonal and biotinylated antibodies for immunohistochemical use. The following protocol has been developed
癌胚抗原(Carcinombryonic－antigen－,CEA)
自1965年Gotel等从结肠癌分离出一种肿瘤的相关抗原后，经过进一步的研究后发现，它存在于胎儿的肠里，由于人们将其称为癌胚抗原。在实际应用 中，CEA是一种广谱的标记物。经实验证明，它对胃癌，肠癌，肺癌，乳腺癌，胰腺癌，子宫颈癌，子宫体腺癌，卵巢癌，皮肤鳞状细胞癌，膀胱移行上皮癌，食 道癌
Antigen－Dependent－B－Cell－Activation
A key part of the immune system is the production of immunoglobulins (antibodies) by B cells to bind and inactivate specific foreign antigens. The bod
Immunohistochemistry－on－paraffin－embedded－sections
主要试剂1. Neutral-buffered Formalin, 10% (NBF), 1 liter (Commercially available pre-prepared from many laboratory reagent suppliers).Double -distilled H
ATATES－Genomic－Antigen－Immunotherapy－for－Potential－Cure－of－Cancer
分析测试百科网讯 2015年10月20 日，第十三届国际新药发明科技年会与第五届药物递放系统研讨会（SDDS-2015）于北京希尔顿逸林酒店同期举办并开幕。 BioMedicure公司总裁 钱勇 BioMedicure公司总裁兼首席执行官钱勇带来题目为《ATATES Genomic
Preparation－and－Staining－of－Paraffin－Sections
I. Fixation and Processing of Tissue for Paraffin SectionsA. Fixation of Tissues in 10% Neutral Buffered FormalinSacrifice animal by prescribed and ap
Immunohistochemistry
实验概要The following procedure describes the application of peroxidase or alkaline phosphatase conjugates in the immunohistochemical labeling of forma
immunofluorescence－of－rabbit－antimurine－RELMα－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of rabbit anti-murine RELMα by Peprotech.实验步骤The following protocol used B6 mice
Microwave－citrate－Pretreatment－of－Paraffin－Sections
1.Deparaffinize slides a (after drying thoroughly overnight at RT) in 2 changes of xylene (or xylene substitute) for 10 mins each.2.Transfer slides to
组织学——微波技术
Microwave Processing Techniques for Microscopy (Energy Beam Sciences, Inc.)Introduction to microwave technique and detailed protocol for its applicati
immunofluorescence－of－rabbit－antimurine－VEGF－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of rabbit anti-murine VEGF by Peprotech.实验步骤The following protocol used normal muri
可溶性抗原(soluble－antigen)的制备及鉴定
蛋白质、糖蛋白、脂蛋白、酶类、补体、脂多糖、细菌外毒素和核酸等均为可溶性抗原，它们有相当部分来源于组织和细胞，成分复杂。制备这类免疫原时，首先须将组织和细胞破碎，然后再从组织和细胞匀浆中提取目的蛋白或其他抗原，提纯的抗原需鉴定后才能用做免疫原。 一、组织匀浆的制备用于制备免疫原的材料必须是新鲜或低温
immunofluorescence－of－rabbit－antimurine－leptin－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of rabbit anti-murine leptin by Peprotech.实验步骤The following protocol used Trichur
Immunohistochemistr...
实验概要Peprotech provides a immunohistochemistry protocol for Mouse Anti-Human RANTES.实验步骤The following protocol used formalin-fixed paraffin-embedded hu
immunofluorescence－of－rabbit－antimurine－RELMβ－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of rabbit anti-murine RELMβ by Peprotech.实验步骤The following protocol used B6 mice
immunohistochemistry－of－mouse－antihuman－Eotaxin2－by－Peprotech
实验概要The following protocol provides a method of immunohistochemistry of mouse anti-human Eotaxin-2 by Peprotech.实验步骤The following protocol used formal
Immunohistochemistr...
实验概要Peprotech provides a immunohistochemistry protocol for Mouse Anti-Human MCP-1*.实验步骤The following protocol used formalin-fixed paraffin-embeddedhum
immunofluorescence－of－mouse－antihuman－IL6－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of mouse anti-human IL-6 by Peprotech.实验步骤The following protocol used human endomet
免疫组织化学
· Double Peroxidase (HRP) Immunohistochemical Labeling of Trypsin-Sensitive Antigens (KPL)· Immunohistochemistry (Tyner lab)This is a
Mouse－hepatitis－B－virus－e－antigen,HBeAg－ELISA－Kit使用说明
Mouse hepatitis B virus e antigen,HBeAg ELISA Kit使用原理小鼠乙型肝炎e抗原(HBeAg)ELISA试剂盒货号:FK-QZ2967规格：96T/48TMouse hepatitis B virus e antigen,HBeAg ELISA Kit
TUNEL－assay
PROTOCOL:•Deparaffinize and rehydrate slides:3 x 3´ Xylene3 x 2´ 100% ethanol1 x 2´ 95%, 80%, 70% ethanol (each)1 x 5´ 1x PBS•Microwave antigen retrie
Preparing－cells－and...
实验概要The method provides a protocol and tips for BrdU staining in tissue sections.Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic
Mouse－hepatitis－B－virus－e－antigen,HBeAg－ELISA－Kit使用说明书
供应商： 上海樊克生物科技有限公司 数量： 100 规格： 96T/48T Mouse hepatitis B virus e antigen,HBeAg ELISA Kit使用原理 小鼠乙型肝炎e抗原(HBeAg)ELISA试剂盒 货号:FK-QZ2967
Mouse－hepatitis－B－virus－e－antigen,HBeAg－ELISA－Kit使用说明书
供应商： 上海樊克生物科技有限公司 数量： 100 规格： 96T/48T Mouse hepatitis B virus e antigen,HBeAg ELISA Kit使用原理 小鼠乙型肝炎e抗原(HBeAg)ELISA试剂盒 货号:FK-QZ2967
Mouse－hepatitis－B－virus－e－antigen,HBeAg－ELISA－Kit使用说明书
供应商： 上海樊克生物科技有限公司 数量： 100 规格： 96T/48T Mouse hepatitis B virus e antigen,HBeAg ELISA Kit使用原理 小鼠乙型肝炎e抗原(HBeAg)ELISA试剂盒 货号:FK-QZ2967