Zebrafishwholemou...
实验概要Whole mount staining of Zebrafish embryos, now commonly used, requires extra steps to fix and permeabilize to ensure the egg membrane is permeabilized. Incubations for fixative, blocking buffer, antibody, wash buffer, permeabilization and substrate color development will need to be much longer than normal immunocytochemistry /immunohistochemistry to allow for permeabilization right into the centre of ......阅读全文
Zebrafish－whole－mou...
实验概要Whole mount staining of Zebrafish embryos, now commonly used, requires extra steps to fix and permeabilize to ensure the egg membrane is perme
Zebrafish－Staging
In order to repeat a published experiment, or have someone else repeat yours, it is important to use the same materials. For developmental studies, th
Isolation－of－Zebrafish－embryos
Zebrafish will mate and deposit fertilized eggs on the bottom of the tank at 'dawn'. They can be accustomed to lay at any convenient time by k
The－Effects－of－Ethanol－on－Zebrafish－Development
Introduction:The early stages of zebrafish, Danio rerio development are characterized by meroblastic dicoidal cleavage. Initially cell division only o
SDS－Whole－Cell－Extracts
-Use sterile technique and sterile solutions in steps 1 to 3.-1. Using a saturated starter culture, inoculate 25 to 30 ml of appropriate media in a 12
IMMUNOHISTOCHEMISTRY－ON－WHOLE－MOUNT－EMBR
Dechorionate directly on apple juice/agar plates with 50-60 chlorox; embryos will float to the surface after 2-3''.Collect embryos on a Millip
Immunofluorescence－Whole－Mount
Objective:Immunohistochemistry allows visualization of antigens (usually proteins) within an embryo. Typically, a primary antibody binds specifically
Whole－mount－fluores...
实验概要The method provides a protocol for whole mount fluorescent immunohistochemistry. The advantage of using fluorescence to stain whole mount sectio
Separation－of－Platelets－from－Whole－Blood
PurposeThis protocol describes how to isolate human platelets from whole blood. Isolated platelets are used for static adhesion assays, for flow chamb
Serum－Separation－from－Whole－Blood
Serum Separation from Whole Blood1) Collect sample (preferably in glass tubes) and leave for 1 hour at 37°C to allow it to clot.2) Leave sample at 4°C
Ultraviolet－irradiation－impairs－epiboly－via－microtubules－in－Zebrafish
IntroductionZebrafish have transparentembryos that develop outside the mother. They develop rapidly, so that at 24 hours after fertilization, the embr
Early－development－of－primary－motor－neurons－and－somites－in－Zebrafish－Embryos
Background:Zebrafish,or the teleost fish Danio rerio,is a rapidly developing organism that is apopular species for studying vertebrate development. Cl
Whole－mount－TUNEL－analysis－of－Xenopus－embryos
Fixation and pretreatmentDejelly albino embryos carefully in 2% Cystein (pH 7.8).Remove the vitellin membrane with two pairs of tweezers (or c
Protein－extraction－from－whole－tissues－for－IEF
Modified from that of Jay Thelen - University of Missouri-ColumbiaPhenol extraction followed by methanolic ammonium acetate precipitation - an effecti
高通量研究脊椎动物多维表型的方法（二）
3.颅面畸形三维重建与评估：每条斑马鱼幼体，记录高达320帧的图像，通过三维重构技术，形象化展示了斑马鱼幼体软骨复杂表型。图像显示的层析效果图充分显示了三种不同药物处理后的5dpf斑马鱼幼体的颅面骨骼变化。三种药物是：retinoic acid（视黄酸）、cyclopamine（环巴胺）和flu
Standard－Protocol－For－Up－to－10－ml－Whole－Blood
实验概要The E.Z.N.A.® Blood DNA Maxi Kit is designed for isolation of genomic DNA from up to 25 ml of fresh, whole blood treated with any common anticoa
Small－Scale－Yeast－Whole－Cell－Extract－for－IP
Grow 100 ml yeast cells in desired media overnight to an A600 of ~1.0 (0.6 to 1.2 works well). For growth in minimal media, 1 ml of a saturated overni
模式生物高通量多维表型研究方法概述（二）
3.颅面畸形三维重建与评估：每条斑马鱼幼体，记录高达320帧的图像，通过三维重构技术，形象化展示了斑马鱼幼体软骨复杂表型。图像显示的层析效果图充分显示了三种不同药物处理后的5dpf斑马鱼幼体的颅面骨骼变化。三种药物是：retinoic acid（视黄酸）、cyclopamine（环巴胺）和flu
Direct－PCR－from－Whole－Yeast－Cells:－Zymolyase－Method
Direct PCR from Whole Yeast Cells: Zymolyase MethodContributor: Namjin ChungDate: June 18, 19961. An average-size yeast colony (0.5-2mm) or a cell pel
Whole－mount－antibody－staining－ofzebrafish－embryos－formarkers－ofsegmentation
1. Dechorionate 26 hr embryos (pharyngula stage) carefully with two fine forceps.Transfer to fixative (1% formaldehyde in PBS). Fix for 1 hour rocking
Method:－Lymphoblastoid－Cell－Lines－from－Frozen－Whole－Blood
Method: Lymphoblastoid Cell Lines from Frozen Whole BloodMay 31, 1990Rosalie VeilePurpose:Blood Samples can be stored frozen as a backup in case an LC
不同硒状态与动物骨骼肌胚后生长之间的关系
近日，华中农业大学水产学院水产品安全保障与品质提升课题组在微量元素硒营养领域取得新进展，研究首次报道了不同硒状态与动物骨骼肌胚后生长之间的关系，相关研究结果为深入认识硒在动物骨骼肌生理中的作用提供了重要数据。 硒是人类及动物的必需微量元素，主要以硒代半胱氨酸形式合成到各种硒蛋白中来发挥其生物学
MaxiYield－protocol－for－10－20－ml－Whole－Blood
实验概要The E.Z.N.A.® Blood DNA Maxi Kit is designed for isolation of genomic DNA from up to 25 ml of fresh, whole blood treated with any common anticoa
SQ－Blood－DNA－Maxi－Protocol－for－410－ml－whole－blood
实验概要The E.Z.N.A.® SQ Blood DNA Kit is designed for isolating high molecular weight genomic DNA from fresh, frozen or anticoagulated whole blood. The
SQ－Blood－DNA－Midi－Protocol－for－500l3ml－whole－blood
实验概要The E.Z.N.A.® SQ Blood DNA Kit is designed for isolating high molecular weight genomic DNA from fresh, frozen or anticoagulated whole blood. The
Effect－of－ethanol－on－development－of－Danio－reriro
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
The－Effect－of－pH－Levels－By－Varying－the－Acidity－of－theExtracellular－Solution
BackgroundZebrafish, Brachydanio rerio, have a distinct embryonic development. The embryo goes through a process called epiboly. Epiboly, as defined i
The－effects－ofdifferent－concentrations－oflithium－chloride－on－thedevelopment
AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular Brachydanio rerio embryos results in
胚胎和成年斑马鱼眼情的组织学准备
INTRODUCTIONThis protocol describes the histological preparation of embryonic and adult zebrafish eyes. The methods described here can be easily adapt
基因组学研究成果让斑马鱼研究“快马加鞭”
基因组学研究成果让斑马鱼研究快马加鞭（Genomics: Zebrafish earns its stripes）作者：谢训卫人类发育，生理功能及疾病发生的过程涉及到成千上万的基因和其变异体，但是大部分的基因和其变异体的功能依然是未知的。过去的20年里，斑马鱼逐渐成为研究人类基因功能的重要模式动物。