InCellELISA
实验概要This protocol is a general protocol for ICE analysis and can be used with any of Abcam's ICE-validated antibodies. Antibodies are available in single quantities or are available in smaller amounts as part of a sample ICE pack. Specific scientific information, background and working concentration for each antibody are detailed in each antibody’s corresponding Data Sheet.实验原理In-Cell ELISA is a qua......阅读全文
In Cell ELISA
实验概要This protocol is a general protocol for ICE analysis and can be used with any of Abcam's ICE-validated antibodies. Antibodies are availabl
Cell death detection in Xenopus embryos by ELISA
Sample preparation Wash embryos 1 x in 25% MMR Remove excess buffer Add 10 volumes "incubation buffer", i.e. 50 µl for 5 embryos Lyse the embryos
Cell-based ELISA for primary cells
1. The 96-well micro-plates are pre-coated with extra cellular matrix.2. Primary cells are grown in the 96-well plates. Seed 100μl of 10,000-20,000
Cell-Based ELISA简介以及应用
1、Cell-Based ELISA 的优点： Cell-Based ELISA（基于细胞的 ELISA）是一种全新的ELISA技术，有两个最突出的优点：1.1不需要抽提蛋白、包被微孔板：细胞直接在微孔板里培养，待检测的时候，将细胞固定在微孔板上并进行通透处理即可。这样就避免了抽提蛋白时，由于客观和
Cell-Based ELISA简介以及应用
1、Cell-Based ELISA 的优点： Cell-Based ELISA（基于细胞的 ELISA）是一种全新的ELISA技术，有两个最突出的优点： 1.1不需要抽提蛋白、包被微孔板：细胞直接在微孔板里培养，待检测的时候，将细胞固定在微孔板上并进行通透处理即可。这样就避免
Cell Stem Cell
|泛素连接酶HECTD1通过协调核糖体大小亚基的组装来调控造血干细胞的再生能力 造血干细胞（Hematopoietic stem cell， HSC）具有自我更新和分化的功能。在正常生理状态下，HSC处于休眠状态，其蛋白合成速度受到严格地调控并维持在较低水平。当机体受到损伤时，HSC 能够迅速
Cell to Cell Adhesion Signaling
Interactions between cells responsible for cell to cell adhesion also can communicate signals into the cellular interior, often invol
Cell Thawing/Cell Freezing Protocol
Freezing Cells: Cells should be growing well or known to be in log phase Count, collect and pellet cells in a 15mL test tube Resuspend in fr
Cell Thawing/Cell Freezing Protocol
Freezing Cells:Cells should be growing well or known to be in log phaseCount, collect and pellet cells in a 15mL test tubeResuspend in freezing media
Cell Stem Cell | 连续取得进展！
下丘脑含有惊人的异质性神经元，可调节内分泌，自主神经和行为功能。然而，其分子发育轨迹和神经元多样性的起源仍不清楚。2021年4月21日，中国科学院遗传与发育生物学研究所吴青峰团队在Cell Stem 在线发表题为“Cascade diversification directs generati