CellThawing／CellFreezingProtocol
Freezing Cells:Cells should be growing well or known to be in log phaseCount, collect and pellet cells in a 15mL test tubeResuspend in freezing media so that the concentration is no more than 5x10^6 cells/mL of cold freezing mediaTransfer 1mL of cells to appropriately labelled cryovials and maintain on ice for approximately 30minutesTransfer vials to -80C freezer for 24hrsTransfer to liquid nitrogen dewar or -140C fr......阅读全文
Cell－Thawing/Cell－Freezing－Protocol
Freezing Cells:Cells should be growing well or known to be in log phaseCount, collect and pellet cells in a 15mL test tubeResuspend in freezing media
Cell－Thawing/Cell－Freezing－Protocol
Freezing Cells:Cells should be growing well or known to be in log phaseCount, collect and pellet cells in a 15mL test tubeResuspend in freezing media
Freezing－and－Thawing－of－Mammalian－Cell－Lines
For long term storage of myeloma cells, hybridoma cells, T cells, and other mammalian cell lines in liquid nitrogen, and restoring them in culture.
ES－and－TS－cell－freezing/thawing
Needed: ES cell freezing medium (2x) 2x ES cell freezing medium should be made up fresh each time it is to be used, and should comprise freshly pre
ES－and－TS－cell－freezing/thawing
实验概要ES and TS cell freezing/thawing.主要试剂ES cell freezing medium (2x) 2x ES cell freezing medium should be made up fresh each time it is to be
Protocol－for－Cell－Fusion
Healthy Sp2/0 cells should be rapidly growing by this time. Sp 2/0 cells should be started about two weeks before the cell fusion. Every two days, the
Cell－Extraction－Protocol
实验概要Primary tissues are valuable tools for the study of intracellular and extracellular markers which characterize disease states. We have developed
T－cell－Activation－Protocol
IntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequ
stem－cell－culture－protocol
实验概要stem cell culture protocol主要试剂cell culture supplies and reagentssEnvironment: cell culture requires a sterile environment, so it needs a separat
Cell－Surface－Immunofluorescence－Staining－Protocol
实验概要A method of identifying and enumerating specific cell types in a heterogeneous population of cells by enhancing the specific staining of desired
alamarBlue®－Cell－Viability－Assay－Protocol
实验概要Cell health can be monitored by numerous methods. Plasma membrane integrity, DNA synthesis, DNA content, enzyme activity, presence of ATP, and c
PrestoBlue™－Cell－Viability－Reagent－Protocol
实验概要PrestoBlue™ Cell Viability Reagent is a ready‐to‐use reagent for rapidly evaluating the viability and proliferation of a wide range of cell type
Human－Embryonic－Stem－(ES)－Cell－Protocols——Freezing－Human－ES－Cells
Collagenase cells for approximately 7 minutes at 37 °C (until edges of colonies are curling up). With a 5 ml pipet, gently pipet and scrape colon
Cell－Cycle－Staining－ProtocolDAPI
1. Harvest cells- wash 2X in PBS to get rid of serum proteins. 1200rpm, 5 min2. Resuspend pellet (up to 3x106 cells) in 1.2 ml PBS (Ca and Mg free).3.
Protocol－to－Count－Cell－Number－of－Preimplantation－Embryos
Protocol to Count Cell Number of Preimplantation Embryos using Nuclear Staining with Hoechst 33342 or DAPI Introduction The following is a simple pro
细胞培养——细胞保藏
Working Cell Bank (Contributed by Nanci Donacki)Provides detailed protocol for establishing a working cell bank Master Cell Bank (Contributed by Na
Freezing－Cells
1) Keep prepared solutions on ice.2) Determine total cell count of cells to be frozen. (e.g. 1 X 108 )3) Determine number of vials to be frozen. (e.g.
Cryopreservation－of－Cell－Lines
AimThe protocol below describes the use of passive methods involving an electric -80ºC freezer for the cryopreservation of cell cultures. ECACC routin
Freezing－and－Thawing－of－MEFs
Author: Shalini Jain and Hariom YadavAffiliation: Animal Biochemistry Division, National Dairy Research Institute, Karnal-132001, Haryana, IndiaDate A
Freezing－and－Thawing－cells
Freezing and Thawing cellsFreezingIt is best to freeze cells that are growing rapidly. With adherent cells, it is easiest to set up 100 mm dishes, giv
Revised－protocol－for－establishing－a－mammary－cell－line－from－tumors
Revised protocol for establishing a mammary cell line from tumors Drench mouse in 70% ethanol, pin and dissect open Sterile dissection of tumor- l
胚胎干细胞培养
Media and Solution required for ES Cell Culture (Bowtell Lab) Routine Culturing of ES Cells (Bowtell Lab) Routine Splitting and freezing of cells (
Routine－Splitting－and－freezing－of－cells
1. Grow cells to subconfluence in a flask. 2. Harvest as per normal and count. 3. Spin down 5min 1.2K in benchtop. Resuspend at 1.0 X 106/ml in 10%
Freezing－cells－in－liquid－nitrogen
Take off MediaTrypsinate with 1ml x2 Dulbecco A trypsinAdd 7ml MediaPipette up and down to distribute cells throughout media (i.e. not clumped togethe
Cryopreserving－Neural－Stem－Cells
实验概要There are numerous protocols available for cryopreserving neural stem cells (NSCs) derived from human embryonic stem cells; the primary objectiv
The－OP9DL1－System:－Generation－of－TLymphocytes－from－Embryonic1
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zúñiga-Pflücker1Sunn
ES－Cell－Culture－and－Manipulation2
Picking ES cell clonesOne or two days before picking colonies prepare 24-well plates of feeders. You can also use alternate protocols that utilize 96-
病毒冷冻保藏技术
实验概要Snap freezing, or flash freezing, is the process by which samples are lowered to temperatures below -70°C very rapidly using dry ice or liquid
Culturing－Mouse－Embryonic－Fibroblasts
MaterialsTrypsin (Gibco 25200-023)3T3 Medium: 500 mL DME (Invitrogen) + 50 mL FBS (Hyclone) + 5 mL 100x Pen/Strep2x Freezing Medium: 3T3 Medium + 20%
巨噬细胞和单核白细胞
· Lymphocyte Transformation (Donis-Keller lab)Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocytes) from antico