TransplantationoftheeyeformingregionofAmphibianneurulaintotheflank
EMBRYO CELL AND TISSUE CULTURE TECHNIQUESRearing solution: 10% Modified Steinberg's Solution. Operating solution:Full strength HBSt solution.Microsurgery Preparation1)Place two embryos in the 60 mm petri dish containing Operating solution with antibiotics.2)Using tweezers and forceps dejelly the embryo and remove the membrane around it.3)Prepare 2% agarose-coated operating dishes in 1X HBSt (+/- powdered cha......阅读全文
Transplantation of theeye-forming region of Amphibian neurula into theflank
EMBRYO CELL AND TISSUE CULTURE TECHNIQUES Rearing solution: 10% Modified Steinberg's Solution. Operating solution:Full strength HBSt solutio
Fate Mapping Axolotl Embryos in Early Gastrulation
BackgroundSince different amphibians execute gastrulation in different ways, the study of amphibian gastrulation has been complex. A popular method us
Developmental and phenotypic implications of transplantation
Objective In this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate
Axolotl Protocol: Transplantation of the Gill Organ Field
Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ fie
Murine Skin Transplantation
Prior to the initiation of experiment:Obtain IACUC approvalSterilize surgical instruments and Q-tipsI. Harvest Donor TissueEuthanize donor mouse (in a
Developmental Effects of Transplantation of Cell
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mo
Dorsal Lip Transplant in Amphibian Embryos
IntroductionIn early stages of development in amphibian embryos, very few cells' fates are rigidly determined. However, previous experiments show
Yale University Policy for Aseptic Amphibian Survival Surgery
Background: Aseptic surgery by definition is surgery performed without contamination or exposure to pathogens. According to the AWR's and the Guid
Regulative Development in Axolotl Embryos; Splitting the Heart Field
ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically, we will split the morphogenetic
Development of the Heart Morphogenetic Field in the Axolotl Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d