WIKI资讯
WIKI资讯
The polymerase chain reaction (PCR) is a method for amplifying specific segments of DNA defined by the small primers used to start the reaction. Using arbitrarily chosen 10-base primers, one can generate "random amplified polymorphic DNA" (RAPD) markers (Williams et al. 1991 Nucl. Acids Res. 18:6531-6535). These DNA fragments, separated by electrophoresis in an agarose gel, can be used as markers for studyi......阅读全文
DNA抽提（主要内容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
实验概要The PureLink™ Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m
实验概要 The ChargeSwitch® gDNA Mini and Micro Tissue Kits allow rapid and efficient purification of genomic DNA from mini (10-25 mg) or
· Standard PCR Protocol (Molecular Biology Techniques Manual)The followings are described in
主要内容如下：· RT-PCR· Competitive and Quantative
实验概要The iPrep™ GeneCatcher™ gDNA Blood Kit allows rapid and automated extraction of genomic DNA (gDNA) from human blood including archived
实验概要 This protocol utilizes the powerful guanidine isothiocyanate–phenol:chloroform extraction method which allows the
N.M. DuTeau and J.F. Leslie - Department of Plant Pathology, Throckmorton Hall, Kansas State University, Manhattan, KS 66506-5502The polymerase chain
We have found that the DNA extraction procedure of Metzenberg and Baitch (Neurospora Newsl. 28:20)/Stevens and Metzenberg (Neurospora Newsl. 29:27) wh
DNA的酶学操作DNA Modifying Enzymes (Michael Blaber)Introduction to bacterial restriction/modification system. It provides very useful background knowl
实验概要The SuperScript™ III One-Step RT-PCR System with Platinum® Taq High Fidelity is designed for sensitive, high-fidelity end-p
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC.
实验概要Plant DNAzol® is an extra-strength-DNAzol® reagent (patent pending) specifically formulated for the isolation of genomic DNA from plants. The Plan
实验概要The E.Z.N.A.® Blood DNA Maxi Kit is designed for isolation of genomic DNA from up to 25 ml of fresh, whole blood treated with any comm
实验概要DNAzol® Reagent (Genomic DNA Isolation Reagent) is a complete and ready-to-use reagent for the isolation of genomic DNA from solid and
Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type IQuantizatio
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Cor
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
2 目前国内外应用于检测耐药结核杆菌的分子生物学方法随着分子生物学的发展，尤其是PCR技术的问世，检测耐药可以直接从基因入手，这样与传统的结核杆菌药敏试验相比，不仅大大缩短了检测周期，实现了自动化，而且也降低了生物实验室的危险性。耐药检测包括用PCR扩增基因组内携带的耐药性区域，和进行扩增产物的突变
实验概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin &nb
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and reci
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and reci
Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA, such as chemical synthe
Preparation of fungi for DNA extraction typically involves growing cultures in liquid culture in Erlenmeyer flasks, Roux bottles or even microfuge tub
MaterialsTrypsin (Gibco 25200-023)3T3 Medium: 500 mL DME (Invitrogen) + 50 mL FBS (Hyclone) + 5 mL 100x Pen/Strep2x Freezing Medium: 3T3 Medium
Andrea J. Gossett and Jason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Correspon
实验概要 Extension of overlapping gene segments by PCR is a simple, versatile technique for site-directed mutagenesis and g
实验概要The ChargeSwitch® PCR Clean-Up Kit allows rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-n
实验概要Intended UseDynabeads® Streptavidin are ideal for numerous applications, including purification of proteins, nucleic acids purificatio