apipelinefortheidentificationofintactNglycopeptides（七）
Complementary ion information provided by HCD- and CID-MS/MS. Both HCD- and CID-MS/MScould be used to optimize the glycopeptide identification. Recently reported software tools used HCD- or CID-MS/MS to identify glycopeptides, especially the glycan moiety of glycopeptides, but few of them integrated these two fragmentations to further improve the glycopeptide identification5,20. GlycoFragWork combined HCDan......阅读全文
a pipeline for the identification of intact N-glycopeptides（七）
Complementary ion information provided by HCD- and CID-MS/MS. Both HCD- and CID-MS/MScould be used to optimize the glycopeptide identification. Rece
a pipeline for the identification of intact N-glycopeptides（二）
Methods Materials. A uniform mixture of six standard glycoproteins was used as the starting material, including IgG (56834, catalog numeber),
a pipeline for the identification of intact N-glycopeptides（五）
Alignment between MS/MS and MS3 identifications. Glycans were identified after analyzing the HCD/CID-MS/MS spectrum pairs, but the sequences of p
a pipeline for the identification of intact N-glycopeptides（六）
Figure 4. Comparison of different decoy strategies. The estimated FDR of each decoy method is compared with the real FDR. “Decoy: + 11” m
a pipeline for the identification of intact N-glycopeptides（一）
pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCDand CID-MS/MS and MS3 Wen-Feng Zeng1,2,*, Ming-Qi Liu3,*, Yang Zhang3,
a pipeline for the identification of intact N-glycopeptides（三）
Figure 1. The overall workflow of pGlyco. First the sample is analyzed by HCD-MS/MS (NCE = 40%).Then the product-dependent CID-MS/MS and data-depend
a pipeline for the identification of intact N-glycopeptides（四）
Interpretation of MS3 data. MS3 data were converted to “.ms3” format by pXtract within pFind Studio (version 2.8)26,27, and then analyzed by pFind
Comprehensive identification of novel proteins and N-glycosylation sites七
Data analysisTandem mass spectra were retrieved using Xcalibur (version 2.2, Thermo Fisher Scientific) and AnalystTF (version 1.6, AB SCIEX) softw
Comprehensive identification of novel proteins and N-glycosylation sites四
Figure 2 Distribution of N-glycopeptides analyzed by different enriched methods and instruments of royal jelly proteins. A is the distribution of N-
An Integrative Procedure for Apoptosis Identification and Measurement
IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr [1]. It plays an important role in embryonic development, maintenance o