CellThawing/CellFreezingProtocol
Freezing Cells:Cells should be growing well or known to be in log phaseCount, collect and pellet cells in a 15mL test tubeResuspend in freezing media so that the concentration is no more than 5x10^6 cells/mL of cold freezing mediaTransfer 1mL of cells to appropriately labelled cryovials and maintain on ice for approximately 30minutesTransfer vials to -80C freezer for 24hrsTransfer to liquid nitrogen dewar or -140C fr......阅读全文
Cell-Thawing/Cell-Freezing-Protocol
Freezing Cells:Cells should be growing well or known to be in log phaseCount, collect and pellet cells in a 15mL test tubeResuspend in freezing media
Cell-Thawing/Cell-Freezing-Protocol
Freezing Cells:Cells should be growing well or known to be in log phaseCount, collect and pellet cells in a 15mL test tubeResuspend in freezing media
Freezing-and-Thawing-of-Mammalian-Cell-Lines
For long term storage of myeloma cells, hybridoma cells, T cells, and other mammalian cell lines in liquid nitrogen, and restoring them in culture.
ES-and-TS-cell-freezing/thawing
Needed: ES cell freezing medium (2x) 2x ES cell freezing medium should be made up fresh each time it is to be used, and should comprise freshly pre
ES-and-TS-cell-freezing/thawing
实验概要ES and TS cell freezing/thawing.主要试剂ES cell freezing medium (2x) 2x ES cell freezing medium should be made up fresh each time it is to be
Protocol-for-Cell-Fusion
Healthy Sp2/0 cells should be rapidly growing by this time. Sp 2/0 cells should be started about two weeks before the cell fusion. Every two days, the
Cell-Extraction-Protocol
实验概要Primary tissues are valuable tools for the study of intracellular and extracellular markers which characterize disease states. We have developed
T-cell-Activation-Protocol
IntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequ
stem-cell-culture-protocol
实验概要stem cell culture protocol主要试剂cell culture supplies and reagentssEnvironment: cell culture requires a sterile environment, so it needs a separat
Cell-Surface-Immunofluorescence-Staining-Protocol
实验概要A method of identifying and enumerating specific cell types in a heterogeneous population of cells by enhancing the specific staining of desired
alamarBlue®-Cell-Viability-Assay-Protocol
实验概要Cell health can be monitored by numerous methods. Plasma membrane integrity, DNA synthesis, DNA content, enzyme activity, presence of ATP, and c
PrestoBlue™-Cell-Viability-Reagent-Protocol
实验概要PrestoBlue™ Cell Viability Reagent is a ready‐to‐use reagent for rapidly evaluating the viability and proliferation of a wide range of cell type
Human-Embryonic-Stem-(ES)-Cell-Protocols——Freezing-Human-ES-Cells
Collagenase cells for approximately 7 minutes at 37 °C (until edges of colonies are curling up). With a 5 ml pipet, gently pipet and scrape colon
Cell-Cycle-Staining-ProtocolDAPI
1. Harvest cells- wash 2X in PBS to get rid of serum proteins. 1200rpm, 5 min2. Resuspend pellet (up to 3x106 cells) in 1.2 ml PBS (Ca and Mg free).3.
Protocol-to-Count-Cell-Number-of-Preimplantation-Embryos
Protocol to Count Cell Number of Preimplantation Embryos using Nuclear Staining with Hoechst 33342 or DAPI Introduction The following is a simple pro
细胞培养——细胞保藏
Working Cell Bank (Contributed by Nanci Donacki)Provides detailed protocol for establishing a working cell bank Master Cell Bank (Contributed by Na
Freezing-Cells
1) Keep prepared solutions on ice.2) Determine total cell count of cells to be frozen. (e.g. 1 X 108 )3) Determine number of vials to be frozen. (e.g.
Cryopreservation-of-Cell-Lines
AimThe protocol below describes the use of passive methods involving an electric -80ºC freezer for the cryopreservation of cell cultures. ECACC routin
Freezing-and-Thawing-of-MEFs
Author: Shalini Jain and Hariom YadavAffiliation: Animal Biochemistry Division, National Dairy Research Institute, Karnal-132001, Haryana, IndiaDate A
Freezing-and-Thawing-cells
Freezing and Thawing cellsFreezingIt is best to freeze cells that are growing rapidly. With adherent cells, it is easiest to set up 100 mm dishes, giv
Revised-protocol-for-establishing-a-mammary-cell-line-from-tumors
Revised protocol for establishing a mammary cell line from tumors Drench mouse in 70% ethanol, pin and dissect open Sterile dissection of tumor- l
胚胎干细胞培养
Media and Solution required for ES Cell Culture (Bowtell Lab) Routine Culturing of ES Cells (Bowtell Lab) Routine Splitting and freezing of cells (
Routine-Splitting-and-freezing-of-cells
1. Grow cells to subconfluence in a flask. 2. Harvest as per normal and count. 3. Spin down 5min 1.2K in benchtop. Resuspend at 1.0 X 106/ml in 10%
Freezing-cells-in-liquid-nitrogen
Take off MediaTrypsinate with 1ml x2 Dulbecco A trypsinAdd 7ml MediaPipette up and down to distribute cells throughout media (i.e. not clumped togethe
Cryopreserving-Neural-Stem-Cells
实验概要There are numerous protocols available for cryopreserving neural stem cells (NSCs) derived from human embryonic stem cells; the primary objectiv
The-OP9DL1-System:-Generation-of-TLymphocytes-from-Embryonic1
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zúñiga-Pflücker1Sunn
ES-Cell-Culture-and-Manipulation2
Picking ES cell clonesOne or two days before picking colonies prepare 24-well plates of feeders. You can also use alternate protocols that utilize 96-
病毒冷冻保藏技术
实验概要Snap freezing, or flash freezing, is the process by which samples are lowered to temperatures below -70°C very rapidly using dry ice or liquid
Culturing-Mouse-Embryonic-Fibroblasts
MaterialsTrypsin (Gibco 25200-023)3T3 Medium: 500 mL DME (Invitrogen) + 50 mL FBS (Hyclone) + 5 mL 100x Pen/Strep2x Freezing Medium: 3T3 Medium + 20%
巨噬细胞和单核白细胞
· Lymphocyte Transformation (Donis-Keller lab)Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocytes) from antico