WIKI资讯
WIKI资讯
实验概要Primary tissues are valuable tools for the study of intracellular and extracellular markers which characterize disease states. We have developed a protocol for rapid isolation of cytokines and signaling molecules from intact tissue. This method is for total protein extraction and makes use of a non-abrasive tissue extraction reagent. Tissue samples as little as 10 mg may be ext......阅读全文
DNA抽提（主要内容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC.
General InformationPhenol/chloroform extraction is an easy way to remove proteins from your nucleic acid samples and can be carried out in a manner th
DNA电泳（主要内容如下） Preparation of Agarose Gel and Electrophoresis Extraction of DNA From Agarose Gel Extraction of DNA fro
A. Phenol extraction of DNA samplesPhenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated
Laser Capture Microdissection (LCM)Introduction to LCM (BJMU) Preparation, LCM and RNA/DNA extraction of Frozen Tissue Sections&
edited by Bruce A. Roe &n
DNA Extraction· DNA Extraction from Bacteria (Julie B. Wolf,UMBC)Phenol/chloroform method·&n
实验概要The procedure presented below describes a method for extracting nuclear from several cell lines of human origin.主要试剂Hypotonic Buffer Solution20 mM
微循环固相微萃取技术检测甲基苯丙胺合成过程中的特征物质云南毗邻世界毒源地 “ 金三角” , 边境线长达4060公里, 特殊的地理位置使其成为境外毒品流入中国的重要通道。随着全球毒品问题的持续泛滥, 新型合成毒品缴获量已远远超过海洛因等传统毒品。由于我国是化工原料生产大国, 云南又成为合成毒品原料非法
Formaldehyde FixationFix in 4% formaldehyde (16% stock EM grade) in CBS for 20 minutesRinse in TBSPermeabilize as for methanol fixationProcede as for
Actin CytoskeletonMethanol fixationFix in -20oC methanol for 1-2.5 minutesRinse in TBSPermeabilize in TBS-0.5% TX for 10 minutesRinse in TBS-0.1% TX (
谷子是中国北方主栽作物之一, 具有节水、抗旱、耐瘠等特点, 对于缓解北方地区水资源贫乏, 提高山区及半山区土地综合利用具有重大意义。目前, 谷子主要以原粮进行加工, 熬粥是其主要的食用方式, 消费形式单一, 市场拉动力不足, 在一定程度上限制了谷子产业的发展[1]。酿造是谷子重要的深加工技术, 近年
Generation of a Light Curve To address the hypothesis concerning photosynthetic efficiency it is necessary to expose sun and shade leaves to a ran
RNA提取（主要内容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Proc
实验概要The Dynabeads® and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface
My Experience Purifying RNA from E. coliRegarding RNA extraction, there is a horrible tendency of people to use kits for RNA extraction with bacteria.
实验概要The Dynabeads® and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface
实验概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). T
1) Immunocapture stageCoating buffer: 15 mM Na2CO3; 35 mM NaHCO3, and 3 mM NaN3, per liter (pH 9.6).Extraction buffer: (20 mM Tris-HCL (pH 8.0),
A Method for Micro-Scale Isolation and Purification of GangliosidesStephan Ladisch~Director, Center for Cancer and Transplant Biology, Children's
1) Immunocapture stageCoating buffer: 15 mM Na2CO3; 35 mM NaHCO3, and 3 mM NaN3, per liter (pH 9.6).Extraction buffer: (20 mM Tris-HCL (pH 8.0),
AbstractChIP-Chip stands for Chromatin Immunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genom
实验概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin &nb
实验概要This method is use to extract short RNAs from plant tissue. Some of the variables (e.g. centrifugation speeds×, precipitation
蛋白质提取和纯化（主要内容如下）Protein Extraction Protein PurificationProtein PrecipitationColumn PreparatioinQ & A Posted in the Method ForumProtein Extrac
实验概要Plant DNAzol® is an extra-strength-DNAzol® reagent (patent pending) specifically formulated for the isolation of genomic DNA from plants. The Plan
There has been a recent revival of interest in the use of AA composition for the identification of proteins from 2-D gels. This technique uses the idi
芯片毛细管电泳具有进样量少，灵敏度高，分析速度快等特点，非常适合法医DNA-STR的快速检验。毛细管电泳芯片由于尺寸小，可施加较大场强，所以在几秒钟内就可完成对样品的分离，微阵列毛细管电泳芯片可实现高通量检测则成为目前学者研究的热点。2002年Emrich CA等[31]报道的将高通量384孔毛
一、实验试剂 GUS Buffer (500 ml) 2.0478 g Na2HPO4 1.2688 g NaH2PO4 (=50 mM NaPi pH7.0) 10 ml 0.5 M EDTA (=10 mM) 0.5 g &n